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| 5. |  | MOURA, J. F. P. de; PIMENTA FILHO, E. C.; ALMEIDA, C. C. de. Índices zootécnicos e econômicos relativos à exploração de caprinos, em uma propriedade, no semi-árido paraibano. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., 2003, Santa Maria, RS. Otimizando a produção animal: anais. Santa Maria: Sociedade Brasileira de Zootecnia, 2003. 5 f. 1 CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | ALMEIDA, O. C. de; ALMEIDA, C. C. de; FIGUEIREDO, J. M. de. Obtencao, em meio de cultura, de propagulos de Verticillium dahliae Kleb., causador da murcha-de-verticillium em cacaueiro (Theobroma cacao L.). Agrotropica, Ilheus, v.1, n.3, p.213-215, set./dez. 1989. Nota.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 8. | | PIZAURO, L. J. L.; ALMEIDA, C. C. de; ROSSI JÚNIOR, O. D.; ÁVILA, F. A. de; ZAFALON, L. F. Isolamento de Staphylococcus coagulase-negativos e variação na contagem de células somáticas em amostras de leite bubalino. Revista Brasileira de Higiene e Sanidade Animal, v. 8, n. 5, (Supl. 1), p. 295-305, 2014.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | MELO, P. C.; SOUZA, V. de; ALMEIDA, C. C. de; NADER FILHO, A.; GONTIJO FILHO, P. P. Production of biofilms by Staphylococcus aureus isolates from environmental milking and mastitis cases in different seasons. In: WORLD VETERINARY CONGRESS, 31., 2013, Prague, Czech Republic. Proceedings & abstracts... Prague: World Veterinary Association, 2013. Abstract ID: 367.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | MELO, P. de C.; ALMEIDA, C. C. de; SOUZA, V. de; NADER-FILHO, A.; GONTIJO FILHO, P. P. Epidemiological relationship between staphylococcus aureus strains from cows with bovine mastitis and milking environment by pulsed-field electrophoresis. In: WORLD BUIATRICS CONGRESS, 27., 2012, Lisboa. Proceedings... Lisboa: APB, 2012. Publicado em: Revista Portuguesa de Buiatria - Abstract Book. Lisboa, 2012. Abstract P:438 p. 136-137.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | RIBEIRO, M. N.; PIMENTA FILHO, E. C.; ALMEIDA, C. C. de; COSTA, R. G.; NASCIMENTO, J. C. do. Características físico-químicas da carne de caprinos submetidos a diferentes níveis de substituição do leite por soro de queijo durante o aleitamento. Revista Brasileira de Zootecnia, Viçosa, MG, v. 26, n. 3, p. 595-598, 1997| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | COSTA, R. G.; ALMEIDA, C. C. de; PIMENTA FILHO, E. C.; HOLANDA JUNIOR, E. V.; SANTOS, N. M. dos. Caracterização do sistema de produção caprino e ovino na região semi-árida do estado da Paraíba, Brasil. Archivos de Zootecnia, v. 57, n. 218, p. 195-205, 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ROSSI JUNIOR, O. D.; AVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. Species level identification of coagulase negative Staphylococcus spp. from buffalo using matrix-assisted laser desorption ionization-time of flight mass spectrometry and cydB real-time quantitative PCR. Veterinary Microbiology, v. 204, p. 8-14, 2017.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | UGIETTE, S. M. A.; PIMENTA FILHO, E. C.; COSTA, R. G.; COSTA, I. P. da; RIBEIRO, M. N.; ALMEIDA, C. C. de. Análise econômica da utilização de resíduo de indústria de queijo (soro de leite) em substituição ao leite de cabra na alimentação artificial de cabritos. In: SIMPÓSIO NORDESTINO DE NUTRIÇÃO DE RUMINANTES, 3., 1990, João Pessoa. Anais... João Pessoa: Universidade Federal da Paraíba, 1990. p. 159.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | UGIETTE, S. M. de A.; PIMENTA FILHO, E. C.; COSTA, R. G.; COSTA, I. P. da; RIBEIRO, M. N. R.; ALMEIDA, C. C. de. Análise econômica da utilização de resíduos de indústria de queijo (soro de leite) em substituição ao leite de cabra na alimentação artificial de cabritos. In: SIMPÓSIO NORDESTINO DE ALIMENTAÇÂO DE RUMINANTES, 3., 1990, João Pessoa. Anais... João Pessoa: Universidade Federal da Paraíba, 1990. p. 159.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | PIZAURO, L. J. L.; ALMEIDA, C. C. DE; SILVA, S. R.; MACINNES, J. I.; KROPINSKI, A. M.; ZAFALON, L. F.; AVILA, F. A. DE; VARANI, A. DE M. Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, bioflm, and related regulatory genes. Scientific Reports, v.11, 2021, 17392. 10 p.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 30 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/12/2019 |
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Data da última atualização: |
11/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
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Afiliação: |
Lucas J. L. Pizauro, UNESP; Camila C. de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Fernando A. de Ávila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
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Título: |
Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v.102, n.12, dec. 2019. |
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Páginas: |
p.11459-11464 |
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DOI: |
https://doi.org/10.3168/jds.2018-15920 |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. MenosThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also dete... Mostrar Tudo |
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Palavras-Chave: |
Dairy buffalo; Virulence genes. |
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Thesaurus NAL: |
Antibiotic resistance; Biofilm. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02937naa a2200277 a 4500
001 2116604
005 2019-12-11
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15920$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aDetection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.$h[electronic resource]
260 $c2019
300 $ap.11459-11464
500 $aShort communication.
520 $aThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes.
650 $aAntibiotic resistance
650 $aBiofilm
653 $aDairy buffalo
653 $aVirulence genes
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aÁVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tJournal of Dairy Science$gv.102, n.12, dec. 2019.
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