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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
19/12/2023 |
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Data da última atualização: |
19/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, A. C. A. de; SANTANA, F. V.; OLIVEIRA, L. A. R. de; SILVA, A. V. C. da; AMARAL, A. L. do; LEDO, A. da S. |
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Afiliação: |
ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; FERNANDA VIEIRA SANTANA, UFS; LEILA ALBUQUERQUE RESENDE DE OLIVEIRA, UFS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; ADRIANE LEITE DO AMARAL, CPATC; ANA DA SILVA LEDO, CPATC. |
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Título: |
Cryopreservation of sugarcane species by droplet vitrification. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Foco, v. 16, n. 8, e2792, p. 1-13, 2023. |
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ISSN: |
1981-223X |
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Idioma: |
Inglês |
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Conteúdo: |
Sugarcane (Saccharum spp.) is considered one of the major crops for energy purposes in tropical, semi-tropical and subtropical regions worldwide. The in vitro maintenance of the accessions available in field bank germplasm, has been considered a complementary and security strategy and the cryopreservation methods are used by world germplasm banks to preserve the biodiversity of plant species. The aim of this study was to evaluate the efficacy of the droplet-vitrification technique in two species of Saccharum spp. Shoot tips from MIA 35301 (S. robustum L.) and NSL 291979 (S. spontaneum L.) were excised from in vitro culture in the 2nd subculture. First, different concentrations of sucrose were used on the preculture step for MIA 35301'. After that, the best concentration was used (0.3 M sucrose) and the MIA 35301 shoot tips were exposure to PVS3 and PS3 solutions at different times. For the third experiment, Shoot tips from MIA 35301 and NSL 291979 accessions were precultured and dehydrated in PVS2 and PVS3 at 15, 30 and 45 min, cooled and rewarming in loading solution and transferred to regeneration medium. Preculture in MS culture medium with 0.3 M sucrose and exposure to PVS2 for 45 min is promising for the cryopreservation of MIA 35301 (S. robustum L.) by droplet-vitrification. A cana-de-açúcar (Saccharum spp.) é considerada uma das principais culturas para fins energéticos em regiões tropicais, semitropicais e subtropicais em todo o mundo. A manutenção in vitro de acessos disponíveis em bancos de germoplasma de campo, tem sido considerada uma estratégia complementar e segura. E a criopreservação tem sido utilizada em bancos de germoplasma para preservar a biodiversidade de várias espécies. O objetivo deste estudo foi avaliar a eficácia da técnica de gotícula-vitrificação em duas espécies de Saccharum spp. Meristemas apicais dos acessos MIA 35301 (S. robustum L.) e NSL 291979 (S. spontaneum L.) foram excisados de culturas in vitro no 2º subcultivo. Primeiro, diferentes concentrações de sacarose na etapa de pré-cultura foram avaliadas para o acesso MIA 35301. Em seguida, a melhor concentração de sacarose foi utilizada na fase de pré-cultura e meristemas apicais de MIA 35301 foram desidratados em soluções de vitrificação PVS2 e PVS3 em diferentes tempos. Para o terceiro experimento, meristemas apicais dos acessos MIA 35301 e NSL 291979 após pré-cultura, foram expostas às soluções de PVS2 e PVS3 por 15, 30 e 45 min, submetidos ao nitrogênio líquido, reaquecidos em solução de carregamento e transferidos para meio de regeneração. A pré-cultura de meristemas apicais em meio de cultura MS com 0,3 M sacarose e a exposição ao PVS2 por 45 min são promissoras na criopreservação por vitrificação em gotas do acesso MIA 35301 (S. robustum L.). La caña de azúcar (Saccharum spp.) es considerada uno de los principales cultivos con fines energéticos en las regiones tropicales, semitropicales y subtropicales de todo el mundo. El mantenimiento in vitro de accesiones disponibles en bancos de germoplasma de campo se ha considerado una estrategia complementaria y segura. Y la criopreservación se ha utilizado en bancos de germoplasma para preservar la biodiversidad de varias especies. El objetivo de este estudio fue evaluar la efectividad de la técnica de vitrificación por gotitas en dos especies de Saccharum spp. Los meristemas apicales de las accesiones MIA 35301 (S. robustum L.) y NSL 291979 (S. spontaneum L.) se extirparon de cultivos in vitro en el segundo subcultivo. Primero, se evaluaron diferentes concentraciones de sacarosa en el paso de precultivo para la accesión MIA 35301. Luego, se utilizó la mejor concentración de sacarosa en el paso de precultivo y los meristemos apicales de MIA 35301 se deshidrataron en soluciones de vitrificación PVS2 y PVS3 en diferentes tiempos. Para el tercer experimento, los meristemas apicales de las accesiones MIA 35301 y NSL 291979 después del precultivo se expusieron a soluciones PVS2 y PVS3 durante 15, 30 y 45 min, se sometieron a nitrógeno líquido, se recalentaron en solución de carga y se transfirieron a regeneración. El precultivo de meristemos apicales en medio de cultivo MS con sacarosa 0,3 M y la exposición a PVS2 durante 45 min son prometedores para la crioconservación por vitrificación en gotas de la accesión MIA 35301 (S. robustum L.). MenosSugarcane (Saccharum spp.) is considered one of the major crops for energy purposes in tropical, semi-tropical and subtropical regions worldwide. The in vitro maintenance of the accessions available in field bank germplasm, has been considered a complementary and security strategy and the cryopreservation methods are used by world germplasm banks to preserve the biodiversity of plant species. The aim of this study was to evaluate the efficacy of the droplet-vitrification technique in two species of Saccharum spp. Shoot tips from MIA 35301 (S. robustum L.) and NSL 291979 (S. spontaneum L.) were excised from in vitro culture in the 2nd subculture. First, different concentrations of sucrose were used on the preculture step for MIA 35301'. After that, the best concentration was used (0.3 M sucrose) and the MIA 35301 shoot tips were exposure to PVS3 and PS3 solutions at different times. For the third experiment, Shoot tips from MIA 35301 and NSL 291979 accessions were precultured and dehydrated in PVS2 and PVS3 at 15, 30 and 45 min, cooled and rewarming in loading solution and transferred to regeneration medium. Preculture in MS culture medium with 0.3 M sucrose and exposure to PVS2 for 45 min is promising for the cryopreservation of MIA 35301 (S. robustum L.) by droplet-vitrification. A cana-de-açúcar (Saccharum spp.) é considerada uma das principais culturas para fins energéticos em regiões tropicais, semitropicais e subtropicais em todo o mundo. A manutenção in vitro de acesso... Mostrar Tudo |
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Thesagro: |
Banco de Germoplasma; Cana de Açúcar; Citogenética Vegetal; Cultura In Vitro; Preservação da Natureza. |
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Thesaurus Nal: |
Cryopreservation; Germplasm conservation; In vitro culture; Saccharum; Sugarcane. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160000/1/Cryopreservation-of-sugarcane...2023.pdf
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Marc: |
LEADER 05314naa a2200313 a 4500
001 2160000
005 2023-12-19
008 2023 bl uuuu u00u1 u #d
022 $a1981-223X
100 1 $aOLIVEIRA, A. C. A. de
245 $aCryopreservation of sugarcane species by droplet vitrification.$h[electronic resource]
260 $c2023
520 $aSugarcane (Saccharum spp.) is considered one of the major crops for energy purposes in tropical, semi-tropical and subtropical regions worldwide. The in vitro maintenance of the accessions available in field bank germplasm, has been considered a complementary and security strategy and the cryopreservation methods are used by world germplasm banks to preserve the biodiversity of plant species. The aim of this study was to evaluate the efficacy of the droplet-vitrification technique in two species of Saccharum spp. Shoot tips from MIA 35301 (S. robustum L.) and NSL 291979 (S. spontaneum L.) were excised from in vitro culture in the 2nd subculture. First, different concentrations of sucrose were used on the preculture step for MIA 35301'. After that, the best concentration was used (0.3 M sucrose) and the MIA 35301 shoot tips were exposure to PVS3 and PS3 solutions at different times. For the third experiment, Shoot tips from MIA 35301 and NSL 291979 accessions were precultured and dehydrated in PVS2 and PVS3 at 15, 30 and 45 min, cooled and rewarming in loading solution and transferred to regeneration medium. Preculture in MS culture medium with 0.3 M sucrose and exposure to PVS2 for 45 min is promising for the cryopreservation of MIA 35301 (S. robustum L.) by droplet-vitrification. A cana-de-açúcar (Saccharum spp.) é considerada uma das principais culturas para fins energéticos em regiões tropicais, semitropicais e subtropicais em todo o mundo. A manutenção in vitro de acessos disponíveis em bancos de germoplasma de campo, tem sido considerada uma estratégia complementar e segura. E a criopreservação tem sido utilizada em bancos de germoplasma para preservar a biodiversidade de várias espécies. O objetivo deste estudo foi avaliar a eficácia da técnica de gotícula-vitrificação em duas espécies de Saccharum spp. Meristemas apicais dos acessos MIA 35301 (S. robustum L.) e NSL 291979 (S. spontaneum L.) foram excisados de culturas in vitro no 2º subcultivo. Primeiro, diferentes concentrações de sacarose na etapa de pré-cultura foram avaliadas para o acesso MIA 35301. Em seguida, a melhor concentração de sacarose foi utilizada na fase de pré-cultura e meristemas apicais de MIA 35301 foram desidratados em soluções de vitrificação PVS2 e PVS3 em diferentes tempos. Para o terceiro experimento, meristemas apicais dos acessos MIA 35301 e NSL 291979 após pré-cultura, foram expostas às soluções de PVS2 e PVS3 por 15, 30 e 45 min, submetidos ao nitrogênio líquido, reaquecidos em solução de carregamento e transferidos para meio de regeneração. A pré-cultura de meristemas apicais em meio de cultura MS com 0,3 M sacarose e a exposição ao PVS2 por 45 min são promissoras na criopreservação por vitrificação em gotas do acesso MIA 35301 (S. robustum L.). La caña de azúcar (Saccharum spp.) es considerada uno de los principales cultivos con fines energéticos en las regiones tropicales, semitropicales y subtropicales de todo el mundo. El mantenimiento in vitro de accesiones disponibles en bancos de germoplasma de campo se ha considerado una estrategia complementaria y segura. Y la criopreservación se ha utilizado en bancos de germoplasma para preservar la biodiversidad de varias especies. El objetivo de este estudio fue evaluar la efectividad de la técnica de vitrificación por gotitas en dos especies de Saccharum spp. Los meristemas apicales de las accesiones MIA 35301 (S. robustum L.) y NSL 291979 (S. spontaneum L.) se extirparon de cultivos in vitro en el segundo subcultivo. Primero, se evaluaron diferentes concentraciones de sacarosa en el paso de precultivo para la accesión MIA 35301. Luego, se utilizó la mejor concentración de sacarosa en el paso de precultivo y los meristemos apicales de MIA 35301 se deshidrataron en soluciones de vitrificación PVS2 y PVS3 en diferentes tiempos. Para el tercer experimento, los meristemas apicales de las accesiones MIA 35301 y NSL 291979 después del precultivo se expusieron a soluciones PVS2 y PVS3 durante 15, 30 y 45 min, se sometieron a nitrógeno líquido, se recalentaron en solución de carga y se transfirieron a regeneración. El precultivo de meristemos apicales en medio de cultivo MS con sacarosa 0,3 M y la exposición a PVS2 durante 45 min son prometedores para la crioconservación por vitrificación en gotas de la accesión MIA 35301 (S. robustum L.).
650 $aCryopreservation
650 $aGermplasm conservation
650 $aIn vitro culture
650 $aSaccharum
650 $aSugarcane
650 $aBanco de Germoplasma
650 $aCana de Açúcar
650 $aCitogenética Vegetal
650 $aCultura In Vitro
650 $aPreservação da Natureza
700 1 $aSANTANA, F. V.
700 1 $aOLIVEIRA, L. A. R. de
700 1 $aSILVA, A. V. C. da
700 1 $aAMARAL, A. L. do
700 1 $aLEDO, A. da S.
773 $tRevista Foco$gv. 16, n. 8, e2792, p. 1-13, 2023.
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Embrapa Tabuleiros Costeiros (CPATC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
04/04/2019 |
|
Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, I. C. dos; ALMEIDA, A. F. de; PIROVANI, C. P.; COSTA. M. G. C.; CONCEIÇÃO, A. S. da; SOARES FILHO, W. dos S.; COELHO FILHO, M. A.; GESTEIRA, A. da S. |
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Afiliação: |
IVANILDES CONCEIÇÃO DOS SANTOS, UESC; ALEX-ALAN FURTADO DE ALMEIDA, UESC; CARLOS PRIMINHO PIROVANI, UESC; MARCIO GILBERTO CARDOSO COSTA, UESC; ALESSANDRO SANTOS DA CONCEIÇÃO, UESC; WALTER DOS SANTOS SOARES FILHO, CNPMF; MAURICIO ANTONIO COELHO FILHO, CNPMF; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Physiological, biochemical and molecular responses to drought conditions in field-grown grafted and ungrafted citrus plants. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, v.62, p. 406/420, 2019. |
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Idioma: |
Inglês |
|
Conteúdo: |
The physiological, biochemical and molecular alterations developed by plants under soil water restriction, especially rootstock, is important to elucidate the mechanisms associated to drought tolerance. In order to verify the influence of rootstock and grafting on drought tolerance of citrus plants, we examined water status, photosynthesis, oxidative stress and gene expression in grafted and ungrafted citrus plants subjected to different soil water regimes (well-watered, moderate and severe drought and rehydrated) under field conditions. Analysis were performed in roots and leaves of six plant combinations: ungrafted ?Rangpur Santa Cruz? lime (RL) and ?Sunki Maravilha? mandarin (SM), RL and SM grafted onto themselves (RL/RL and SM/SM) and ?Valencia? sweet orange (VO) grafted onto RL or SM (VO/RL and VO/SM). Drought stress reduced chloroplastid pigment contents and limited the photosynthetic rate, mainly in RL/RL plants. In contrast, the lowest leaf osmotic potential, the best osmotic adjustment and the greater phenotypic plasticity were observed in SM, SM/SMand VO/SM plants. Phenotypic plasticity and the main components of multivariate analysis showed that the physiological variables, such as Fv/F0, F0/Fm and Fv/Fm, were more responsive to soil water deficit. An increase in the superoxide dismutase (SOD) activity and transcript abundance of mitochondrial SOD [Mn-Fe], especially in the scion onto RL, and chloroplastidic SOD [Cu -Zn], inthe scion onto SM, was also observed under water deficit. Besides, an induction of transcription of genes coding for catalase (CAT X2) and ascorbate peroxidase (APX) was also observed, respectively, in leaves and roots of plants subjected to water deficit. However, such transcription increment did not affect the activity of these enzymes, especially in RL/RL plants. RL plants were more effective in soil water extraction than SM plants, evidenced mainly by the maintenance of leaf gas exchange and increase in the leaf relative water content after rehydration. The grafted plants showed alterations in their responses to the stress factor, expressed by a reduction of photosynthetic rates, changes in pigment contents, activity of antioxidant enzymes and expression of genes associated to the antioxidant metabolism, mainly in RL/RL plants. Plants with ?Valencia? scion, regardless of the rootstock studied, showed similar physiological, biochemical and molecular responses when exposed to soil water deficit. Multivariate analysis showed that Fv/F0, F0/Fm and Fv/Fm were the most important physiological variables for discriminating citrus plants subjected to control and soil water deficit conditions. MenosThe physiological, biochemical and molecular alterations developed by plants under soil water restriction, especially rootstock, is important to elucidate the mechanisms associated to drought tolerance. In order to verify the influence of rootstock and grafting on drought tolerance of citrus plants, we examined water status, photosynthesis, oxidative stress and gene expression in grafted and ungrafted citrus plants subjected to different soil water regimes (well-watered, moderate and severe drought and rehydrated) under field conditions. Analysis were performed in roots and leaves of six plant combinations: ungrafted ?Rangpur Santa Cruz? lime (RL) and ?Sunki Maravilha? mandarin (SM), RL and SM grafted onto themselves (RL/RL and SM/SM) and ?Valencia? sweet orange (VO) grafted onto RL or SM (VO/RL and VO/SM). Drought stress reduced chloroplastid pigment contents and limited the photosynthetic rate, mainly in RL/RL plants. In contrast, the lowest leaf osmotic potential, the best osmotic adjustment and the greater phenotypic plasticity were observed in SM, SM/SMand VO/SM plants. Phenotypic plasticity and the main components of multivariate analysis showed that the physiological variables, such as Fv/F0, F0/Fm and Fv/Fm, were more responsive to soil water deficit. An increase in the superoxide dismutase (SOD) activity and transcript abundance of mitochondrial SOD [Mn-Fe], especially in the scion onto RL, and chloroplastidic SOD [Cu -Zn], inthe scion onto SM, was also observed und... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03403naa a2200229 a 4500
001 2107938
005 2019-12-06
008 2019 bl uuuu u00u1 u #d
100 1 $aSANTOS, I. C. dos
245 $aPhysiological, biochemical and molecular responses to drought conditions in field-grown grafted and ungrafted citrus plants.$h[electronic resource]
260 $c2019
520 $aThe physiological, biochemical and molecular alterations developed by plants under soil water restriction, especially rootstock, is important to elucidate the mechanisms associated to drought tolerance. In order to verify the influence of rootstock and grafting on drought tolerance of citrus plants, we examined water status, photosynthesis, oxidative stress and gene expression in grafted and ungrafted citrus plants subjected to different soil water regimes (well-watered, moderate and severe drought and rehydrated) under field conditions. Analysis were performed in roots and leaves of six plant combinations: ungrafted ?Rangpur Santa Cruz? lime (RL) and ?Sunki Maravilha? mandarin (SM), RL and SM grafted onto themselves (RL/RL and SM/SM) and ?Valencia? sweet orange (VO) grafted onto RL or SM (VO/RL and VO/SM). Drought stress reduced chloroplastid pigment contents and limited the photosynthetic rate, mainly in RL/RL plants. In contrast, the lowest leaf osmotic potential, the best osmotic adjustment and the greater phenotypic plasticity were observed in SM, SM/SMand VO/SM plants. Phenotypic plasticity and the main components of multivariate analysis showed that the physiological variables, such as Fv/F0, F0/Fm and Fv/Fm, were more responsive to soil water deficit. An increase in the superoxide dismutase (SOD) activity and transcript abundance of mitochondrial SOD [Mn-Fe], especially in the scion onto RL, and chloroplastidic SOD [Cu -Zn], inthe scion onto SM, was also observed under water deficit. Besides, an induction of transcription of genes coding for catalase (CAT X2) and ascorbate peroxidase (APX) was also observed, respectively, in leaves and roots of plants subjected to water deficit. However, such transcription increment did not affect the activity of these enzymes, especially in RL/RL plants. RL plants were more effective in soil water extraction than SM plants, evidenced mainly by the maintenance of leaf gas exchange and increase in the leaf relative water content after rehydration. The grafted plants showed alterations in their responses to the stress factor, expressed by a reduction of photosynthetic rates, changes in pigment contents, activity of antioxidant enzymes and expression of genes associated to the antioxidant metabolism, mainly in RL/RL plants. Plants with ?Valencia? scion, regardless of the rootstock studied, showed similar physiological, biochemical and molecular responses when exposed to soil water deficit. Multivariate analysis showed that Fv/F0, F0/Fm and Fv/Fm were the most important physiological variables for discriminating citrus plants subjected to control and soil water deficit conditions.
650 $aCitrus
650 $aFruta Cítrica
700 1 $aALMEIDA, A. F. de
700 1 $aPIROVANI, C. P.
700 1 $aCOSTA. M. G. C.
700 1 $aCONCEIÇÃO, A. S. da
700 1 $aSOARES FILHO, W. dos S.
700 1 $aCOELHO FILHO, M. A.
700 1 $aGESTEIRA, A. da S.
773 $tEnvironmental and Experimental Botany$gv.62, p. 406/420, 2019.
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