|
|
![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
26/02/2013 |
Data da última atualização: |
24/06/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
NOGUEIRA, F. C.; SILVA, C. P.; ALEXANDRE, D.; SAMUELS, R. I; SOARES, E. L.; ARAGAO, F. J. L.; PALMISANO, G.; DOMONT, G. B.; ROEPSTORFF, P.; CAMPOS, F. A. |
Afiliação: |
Universidade Federal do Rio de Janeiro; Universidade Federal de Santa Catarina; Universidade Federal de Santa Catarina; Universidade Estadual do Norte Fluminense; Universidade Federal do Ceará; FRANCISCO JOSE LIMA ARAGAO, CENARGEN; University of Southern Denmark; Universidade Federal do Rio de Janeiro; University of Southern Denmark; Universidade Federal do Ceará. |
Título: |
Global proteome changes in larvae of Callosobruchus maculatus Coleoptera:Chrysomelidae:Bruchinae) following ingestion of a cysteine proteinase inhibitor. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Proteomics, v. 12, n. 17, p. 2704-2715, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The seed-feeding beetle Callosobruchus maculatus is an important cowpea pest (Vigna unguiculata) as well as an interesting model to study insect digestive physiology. The larvae of C. maculatus rely on cysteine and aspartic peptidases to digest proteins in their diet. In this work, the global proteomic changes induced in the intestinal tract of larval C. maculatus challenged by the ingestion of cystatin, a cysteine peptidase inhibitor, was investigated by a nanoLC-MS/MS approach. The ingestion of cystatin caused a delay in the development of the larvae, but the mortality was not high, indicating that C. maculatus is able to adapt to this inhibitor. This proteomic strategy resulted in the identification of 752 and 550 protein groups in the midgut epithelia and midgut contents, respectively, and quantitative analyses allowed us to establish relative differences of the identified proteins. Ingestion of cystatin led to significant changes in the proteome of both the midgut epithelia and midgut contents. We have observed that proteins related to plant cell wall degradation, particularly the key glycoside hydrolases of the families GH5 (endo-?-1,4-mannanase) and GH 28 (polygalacturonase) were overexpressed. Conversely, ?-amylases were downexpressed, indicating that an increase in hemicelluloses digestion helps the larvae to cope with the challenge of cystatin ingestion. Furthermore, a number of proteins associated with transcription/translation and antistress reactions were among the cystatin-responsive proteins, implying that a substantial rearrangement in the proteome occurred in C. maculatus exposed to the inhibitor. MenosThe seed-feeding beetle Callosobruchus maculatus is an important cowpea pest (Vigna unguiculata) as well as an interesting model to study insect digestive physiology. The larvae of C. maculatus rely on cysteine and aspartic peptidases to digest proteins in their diet. In this work, the global proteomic changes induced in the intestinal tract of larval C. maculatus challenged by the ingestion of cystatin, a cysteine peptidase inhibitor, was investigated by a nanoLC-MS/MS approach. The ingestion of cystatin caused a delay in the development of the larvae, but the mortality was not high, indicating that C. maculatus is able to adapt to this inhibitor. This proteomic strategy resulted in the identification of 752 and 550 protein groups in the midgut epithelia and midgut contents, respectively, and quantitative analyses allowed us to establish relative differences of the identified proteins. Ingestion of cystatin led to significant changes in the proteome of both the midgut epithelia and midgut contents. We have observed that proteins related to plant cell wall degradation, particularly the key glycoside hydrolases of the families GH5 (endo-?-1,4-mannanase) and GH 28 (polygalacturonase) were overexpressed. Conversely, ?-amylases were downexpressed, indicating that an increase in hemicelluloses digestion helps the larvae to cope with the challenge of cystatin ingestion. Furthermore, a number of proteins associated with transcription/translation and antistress reactions were among ... Mostrar Tudo |
Palavras-Chave: |
Peptidase inhibitors; Plant proteomics. |
Thesagro: |
Callosobruchus Maculatus; Vigna Unguiculata. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02507naa a2200277 a 4500 001 1951245 005 2024-06-24 008 2012 bl uuuu u00u1 u #d 100 1 $aNOGUEIRA, F. C. 245 $aGlobal proteome changes in larvae of Callosobruchus maculatus Coleoptera$bChrysomelidae:Bruchinae) following ingestion of a cysteine proteinase inhibitor.$h[electronic resource] 260 $c2012 520 $aThe seed-feeding beetle Callosobruchus maculatus is an important cowpea pest (Vigna unguiculata) as well as an interesting model to study insect digestive physiology. The larvae of C. maculatus rely on cysteine and aspartic peptidases to digest proteins in their diet. In this work, the global proteomic changes induced in the intestinal tract of larval C. maculatus challenged by the ingestion of cystatin, a cysteine peptidase inhibitor, was investigated by a nanoLC-MS/MS approach. The ingestion of cystatin caused a delay in the development of the larvae, but the mortality was not high, indicating that C. maculatus is able to adapt to this inhibitor. This proteomic strategy resulted in the identification of 752 and 550 protein groups in the midgut epithelia and midgut contents, respectively, and quantitative analyses allowed us to establish relative differences of the identified proteins. Ingestion of cystatin led to significant changes in the proteome of both the midgut epithelia and midgut contents. We have observed that proteins related to plant cell wall degradation, particularly the key glycoside hydrolases of the families GH5 (endo-?-1,4-mannanase) and GH 28 (polygalacturonase) were overexpressed. Conversely, ?-amylases were downexpressed, indicating that an increase in hemicelluloses digestion helps the larvae to cope with the challenge of cystatin ingestion. Furthermore, a number of proteins associated with transcription/translation and antistress reactions were among the cystatin-responsive proteins, implying that a substantial rearrangement in the proteome occurred in C. maculatus exposed to the inhibitor. 650 $aCallosobruchus Maculatus 650 $aVigna Unguiculata 653 $aPeptidase inhibitors 653 $aPlant proteomics 700 1 $aSILVA, C. P. 700 1 $aALEXANDRE, D. 700 1 $aSAMUELS, R. I 700 1 $aSOARES, E. L. 700 1 $aARAGAO, F. J. L. 700 1 $aPALMISANO, G. 700 1 $aDOMONT, G. B. 700 1 $aROEPSTORFF, P. 700 1 $aCAMPOS, F. A. 773 $tProteomics$gv. 12, n. 17, p. 2704-2715, 2012.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
11/07/2014 |
Data da última atualização: |
11/07/2014 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MAGALHAES, F. O. C.; ALLEN, L. N.; FARIA, J. C.; INOUE-NAGATA, A.; HOFFMANN, L.; SUASSUNA, N.; FERREIRA, A.; BARROSO, P. A. |
Afiliação: |
FERNANDA OLIVEIRA DA C MAGALHAES, CNPA; LAISA NOGUEIRA ALLEN, Bolsista DTI CNPq; JOSIAS CORREA DE FARIA, CNPAF; ALICE KAZUKO INOUE NAGATA, CNPH; LUCIA VIEIRA HOFFMANN, CNPA; NELSON DIAS SUASSUNA, CNPA; ALEXANDRE CUNHA DE B FERREIRA, CNPA; PAULO AUGUSTO VIANNA BARROSO, CNPA. |
Título: |
Caracterização parcial de Begomovírus associados ao algodoeiro em Goiás. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DO ALGODÃO, 9., 2013, Brasília, DF. |
Idioma: |
Português |
Conteúdo: |
Viroses do gênero Begomovirus (família Geminiviridae) são transmitidas por moscas brancas. Folhas de plantas de algodoeiro com sintomas típicos de infecção por begomovírus foram coletadas em Santa Helena de Goiás (GO) e Santo Antônio de Goiás (GO) entre 2010 e 2013. Os sintomas observados podem ser descritos como clorose internerval das folhas em mosaico, podendo restringir-se a um setor da planta ou da folha. |
Palavras-Chave: |
Goiás, GO. |
Thesagro: |
Algodão; Doença de planta; Mosca branca; Virus. |
Thesaurus NAL: |
Begomovirus. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/101264/1/BEGOMOVIRUS.pdf
|
Marc: |
LEADER 01188naa a2200277 a 4500 001 1990306 005 2014-07-11 008 2013 bl uuuu u00u1 u #d 100 1 $aMAGALHAES, F. O. C. 245 $aCaracterização parcial de Begomovírus associados ao algodoeiro em Goiás.$h[electronic resource] 260 $c2013 520 $aViroses do gênero Begomovirus (família Geminiviridae) são transmitidas por moscas brancas. Folhas de plantas de algodoeiro com sintomas típicos de infecção por begomovírus foram coletadas em Santa Helena de Goiás (GO) e Santo Antônio de Goiás (GO) entre 2010 e 2013. Os sintomas observados podem ser descritos como clorose internerval das folhas em mosaico, podendo restringir-se a um setor da planta ou da folha. 650 $aBegomovirus 650 $aAlgodão 650 $aDoença de planta 650 $aMosca branca 650 $aVirus 653 $aGoiás, GO 700 1 $aALLEN, L. N. 700 1 $aFARIA, J. C. 700 1 $aINOUE-NAGATA, A. 700 1 $aHOFFMANN, L. 700 1 $aSUASSUNA, N. 700 1 $aFERREIRA, A. 700 1 $aBARROSO, P. A. 773 $tIn: CONGRESSO BRASILEIRO DO ALGODÃO, 9., 2013, Brasília, DF.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|