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Registros recuperados : 16 | |
1. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SANTOS, J. D. R.; ARASHIRO, E. K. N.; BALARO, M. F. A.; SOUZA-FABJAN, J. M. G.; PINTO, P. H. N.; SOUZA, C. V.; ALFRADIQUE, V. A. P.; BADE, A. L. C.; FONSECA, J. F. da; BRANDÃO, F. Z. Cervical transposition test at estrus as a tool to select ewes for transcervical embryo collection. Animal Reprodroduction, v. 15, n. 3, p. 492, Jul./Sept. 2018. Abstracts A110. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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2. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; SOUZA FABJAN, J. M. G.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z. Influence of mini-Percoll techniques in sperm capacitation and plasma membrane integrity of ram frozen-thawed sperm. Animal Reproduction, v. 13, n. 3, p. 552, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,... Biblioteca(s): Embrapa Caprinos e Ovinos. |
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3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; COSENTINO, I. O.; PINTO, P. H. N.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. Dose and administration protocol for FSH used for ovarian stimulation affect gene expression in sheep cumulus-oocyte complexes. Reproduction, Fertility and Development, v. 30, n. 9, p. 1234-1244, 2018. Biblioteca(s): Embrapa Gado de Leite. |
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4. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; ALFRADIQUE, V. A. P.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; ARASHIRO, E. K. N.; BALARO, M. F. A.; OLIVEIRA, I. C. de; BRANDÃO, F. Z.; CÔRTES, L. R.; FONSECA, J. F. da. Efeito de diferentes protocolos hormonais para superestimulação ovariana sobre o número e a qualidade de oócitos em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 470, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ROSA, R. M.; BALARO, M. F. A.; PINTO, P. H. N.; SARAIVA, H. F. R. A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; RIBEIRO, L. S.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. Follicular wave synchronization on in vivo embryo production in Santa Ines sheep. Animal Reproduction, v. 13, n. 3, p. 474, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,... Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; FABJAN, J. M. G. de S.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian simulation alter the gene expression profile in ovine cumulus-oocyte complexes. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 746. Biblioteca(s): Embrapa Gado de Leite. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | OLIVEIRA, C. C. S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; BRANDÃO, F. Z. Mini-Percoll processing of domestic ruminant frozen-thawed semen dispenses the use of heparin in capacitating medium. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 498, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PINTO, P. H. N.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; BRAIR, V. L.; ALFRADIQUE, V. A. P.; CÔRTESA, L. R.; COSENTINO, I. O.; SOUZA-FABJANA, J. M. G.; FONSECA, J. F. da; BRANDÃO, F. Z. Successive in vivo embryo production in Santa Inês sheep. Animal Production Science, v. 60, n. 4, p. 497-502, Dec. 2020. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PINTO, P. H. N.; BRAGANÇA, G. M.; RIBEIRO, L. dos S.; BALARO, M. F. A.; BRAIR, V. L.; CONSENTINO, I. O.; ALFRADIQUE, V. A. P.; FONSECA, J. F. da; BRANDÃO, F. Z. Repetibilidade de resposta para produção in vivo de embriões em ovelhas Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 485, jan./mar. 2017. Resumo apresentado nos anais do XXII Congresso Brasileiro de Reprodução Animal, Santos, SP, 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDX1 expression in in vivo-produced ovine embryos. Animal Reprodroduction, v. 14, n. 3, p. 898, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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13. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. L-carnitine supplementation during vitrification did not improve survival and quality rates, but altered CrAT and PRDXI expression in in vivo-produced ovine embryos. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 901. Biblioteca(s): Embrapa Gado de Leite. |
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14. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression. Theriogenology, v. 105, p. 150-157, Jan. 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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15. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDAO, F. Z. L-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDXI expression. Theriogenology, v. 105, p. 150-157, jan. 2018. Biblioteca(s): Embrapa Gado de Leite. |
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16. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; PINTO, P. H. N.; SANTOS, J. D. R.; CAMARGO, L. S. de A.; MENCHACA, A.; FONSECA, J. F. da; BRANDÃO, F. Z. Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. Domestic Animal Endocrinology, v. 74, e106518, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Registros recuperados : 16 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
23/08/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Gláucia Mota Bragança, Universidade Federal Fluminense (UFF) - Rio de Janeiro, RJ; Ribrio Ivan Tavares Pereira Batista; Joanna Maria Gonçalves de Souza Fabjan; Vivian Angélico Pereira Alfradique; Luana Rangel Côrtes; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandao. |
Título: |
FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. |
Idioma: |
Inglês |
Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. |
Conteúdo: |
Ovarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. MenosOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified in... Mostrar Tudo |
Palavras-Chave: |
Embryo Technology; Expressão gênica; FSH; In vitro embryo production; Produção de embriões in vitro. |
Thesagro: |
Ovino; Reprodução animal; Transferência de embrião. |
Thesaurus NAL: |
Gene expression; Ova; Reproduction; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/162972/1/cnpc-2017-FSH.pdf
|
Marc: |
LEADER 03935nam a2200349 a 4500 001 2074359 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aBRAGANÇA, G. M. 245 $aFSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes.$h[electronic resource] 260 $aAnimal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017.$c2017 500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. 520 $aOvarian stimulation is an important tool to increase the number of oocytes obtained by laparoscopy for the in vitro production of embryos (IVP). In sheep, different concentrations of FSH administered in single dose (SD) or multiple doses (MD) have been adopted. In parallel, the oocyte quality is fundamental for IVP success, so strategies to produce more competent oocytes have been evaluated. The aim of this study was to evaluate the gene expression profile of BCB+ COC from different hormonal protocols of ovarian stimulation in Santa Inês ewes. To achieve that, a cross-over design was used, where 12 pluriparous ewes had their follicular wave synchronized (Balaro et al., Domest Anim Endocrinol, 54: 10-14, 2016). At 80 h after progestogen implant removal, all ewes received a new vaginal sponge and it started the stimulation by administration of: 80 (Group 1 - 80-SD) or 120 (Group 2 - 120-SD) mg FSH (Folltropin-V®, Bioniche Animal Health, Ontario, Canada) and 300 IU of eCG both in single dose, or 80 (Group 3 - 80-MD) or 120 (Group 4 - 120-MD) in decreasing doses (50/30/20%) every 12 h. The COCs were recovered by laparoscopy and classified morphologically in grade I / II (homogeneous ooplasm and more than 3 cumulus cells layers), III (homogeneous ooplasm and less than 3 cumulus cells layers or partially denuded) and IV (heterogeneous ooplasm or degenerate). For inference of the development competence GI, II and III COCs were stained with bright cresyl blue (BCB) and classified into: BCB+ (competent) and BCB- (non-competent). These variables were compared by ANOVA followed by Tukey test. The abundance of mRNA that encodes proteins associated with steroidogenesis (STAR, FSHr, LHr and ER?), oocyte quality (MATER, BMP15, GDF9 and ZAR1) and apoptosis (BAX and Bcl-2) was assessed by real-time qPCR normalized with GAPDH in BCB+ COCs. The abundance of gene transcripts associated with steroidogenesis was down-regulated (P <0.05) with increasing FSH concentration, when administration was performed in a single dose (80-SD and 120-SD). On the other hand, when the administration was performed in MD, only the LHr was down-regulated (80-MD and 120-MD). In the 80-MD group, FSHr and Er? were down-regulated (P <0.05) in comparison with 80-SD. For genes related with oocyte quality, 80-MD showed up-regulation (P <0.05) to MATER (when compared to 80-SD), ZAR1 and MATER (compared to 120-SD). Nonetheless, apoptosis genes were not affected. These data demonstrate that the FSH dose and strategy of administration affect the gene expression profile in ovine COCs. Subsequent studies are necessary to assess the effect of this change on maturation rate and developmental competence. 650 $aGene expression 650 $aOva 650 $aReproduction 650 $aSheep 650 $aOvino 650 $aReprodução animal 650 $aTransferência de embrião 653 $aEmbryo Technology 653 $aExpressão gênica 653 $aFSH 653 $aIn vitro embryo production 653 $aProdução de embriões in vitro 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA FABJAN, J. M. G. de 700 1 $aALFRADIQUE, V. A. P. 700 1 $aCORTÊS, L. R. 700 1 $aCAMARGO, L. S. de A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z.
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