Data/hora: 13/06/2024 07:55:30

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 23/03/2016
Data da última atualização: | 23/03/2016
Autoria: | DONIN, D. G.; LEME, R. de A.; ALFIERI, A. F.; ALBERTON, G. C.; ALFIERI, A. A.
Afiliação: | DAIANE G. DONIN, UFPR; RAQUEL DE A LEME, UEL; ALICE F. ALFIERI, UEL; GERALDO C. ALBERTON, UFPR; AMAURI A. ALFIERI, UFPR.
Título: | Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná State, Brazil.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 403-408, maio 2015.
Idioma: | Inglês
Conteúdo: | Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric icornaviruses in wild boars (Susscrofa scrofa) of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari) as alternative host species for these viruses. Fecal samples (n=36) from asymptomatic wild boars (n=22) with ages ranging from 2 to 7 months old (young, n=14) and 2 to 4 years old (adult, n=8) and from peccaries (6 to 8 months old, n=14) were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR) and nested-PCR (n-PCR) assays targeting the 5?non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50%) detected, followed by PTV (10/22, 45.5%) and PSV (4/22, 18.2%). Regarding the age groups, young wild boars were more frequently (9/14, 64.3%) infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%). One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87.1% to 100% for EV-G. Peccaries tested negative for the viruses and in this study they did not represent infection reservoirs. This study is the first to report the molecular detection of PTV, PSV, and EV-G from captive wild boars in a South American country and the first to screen peccaries as alternative host species for porcine enteric picornavirus.
Palavras-Chave: | Pecari tajacu; PTV; Sapelovírus suíno; Sus scrofa scrofa; Tayassu pecari; Teschovírus suíno.
Thesagro: | Javali.
Thesaurus Nal: | Enterovirus G; Porcine sapelovirus; Porcine teschovirus; Wild boars.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141601/1/Molecular-survey-of-porcine.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59144-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS