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| 5. |  | CERON, J.; COVARRUBIAS, L.; QUINTERO, R.; ORTIZ, A.; ORTIZ, M.; ARANDA, E.; LINA, L.; BRAVO, A. PCR analysis of the cruz I insecticital crytal family genes fron Bacillus thgiringensis. Applied and Environmental microbiology, v.60, n.1, p.353-356, 1994.| Biblioteca(s): Embrapa Algodão. |
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| 6. | | QUEIROZ, P. R.; RAMIRO, C. A.; MARTINS, E. S.; SOBERÓN, M.; BRAVO, A.; PONTES, R. G. M. S. de. Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops. Pesquisa Agropecuária Brasileira, Brasília, DF v. 51, n. 5, p.692-696, maio, 2016. Notas científicas.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 7. | | VALICENTE, F. H.; PAIVA, E.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; SANCHEZ, J.; BRAVO, A. Characterization by PCR analysis of cryl genes from Bacillus Thuringiensis strains efficient against fall armyworm, Spodoptera frugiperda. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 5., 1997, Singapore. Book of abstracts... Singapore: International Society for Plant Molecular Biology, 1997.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | FARINHA, E. T.; DAME, M. C. F.; PRESTES, A. M.; BRAVO, A. P.; MELLO, F. C. B.; RORATO, P. R. N. Parâmetros genéticos para circunferência escrotal para bubalinos da raça Murrah. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 11., 2015, Santa Maria, RS. Melhoramento animal da academia ao campo: uma parceria em construção: anais. Santa Maria, RS: SBMA: UFSM, 2015.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | MARTINS, E.; MACEDO, C.; QUEIROZ, P.; PRACA, L.; SOARES, C. M.; MOREIRA, H.; GRISI, I.; SOBERON, M.; BRAVO, A.; MONNERAT, R. Resistência de Spodoptera frugiperda em cultivo de milho Cry1F. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. Goiânia: Sociedade Entomológica do Brasil, 2014. Resumo 1571.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | SILVA, I. H. S. da; GOMÉZ, I.; SÁNCHEZ, J.; CASTRO, D. L. M. de; VALICENTE, F. H.; SOBERÓN, M.; POLANCZYK, R. A.; BRAVO, A. Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. Plos One, San Francisco, v. 13, n. 12, e0207789, 2018.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | SILVA, I. H. S.; GOMEZ, I.; SANCHEZ, J.; CASTRO, D. L. M.; VALICENTE, F. H.; SOBERON, M.; POLANCZYK, R. A.; BRAVO, A. Identification of midgut membrane proteins from different instars of Helicoverpa armigera that bind to Cry1Ac toxin. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1348.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | PRESTES, A. M.; OLIVEIRA, M. M. de; MELLO, F. C. B.; RORATO, P. R. N.; LOPES, J. S.; FELTES, G. L.; BRAVO, A. P. Genetic evaluation models for post-weaning weight gain in a multibreed Angus-Nelore population. Pesquisa Agropecuária Brasileira, v. 54, e00694, 2019. Título em inglês: Modelos de avaliação genética para ganho de peso pós-desmama em uma população multirracial de Angus-Nelore.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 14. | | SILVA, I. H. S.; NASCIMENTO, J. do; SANCHES, A. C.; DESIDERIO, J. A.; VALICENTE, F. H.; SOBERON, M.; POLANCZYK, R. A.; BRAVO, A. Degradation of Cry1Ac protoxin can explain the decreased susceptibility in late instars of Helicoverpa armigera. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 27.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 10., 2018, Gramado. Saúde, ambiente e agricultura: anais. Gramado: SEB, 2018. p. 1341.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 15. | | MARTINS, E. S.; RIBEIRO, B. M.; QUEIROZ, P. R. M.; DUMAS, V. F.; BRAZ, S. V.; AGUIAR, R. W. S.; BRAVO. A.; PONTES, R. G. M. S. de. Primeiro relato de identificação de receptor para toxinas de Bacillus thuringiensis em Anthonomus grandis. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal, RN. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. p. 126 1 CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | MONNERAT, R.; MARTINS, E.; MACEDO, C.; QUEIROZ, P.; PRAÇA, L.; SOARES, C. M.; MOREIRA, H.; GRISI, I.; SILVA, J.; SOBERON, M.; BRAVO, A. Evidence of field-evolved resistance of Spodoptera frugiperda to Bt corn expressing Cry1F in Brazil that is still sensitive to modified bt toxins. PLoS One, v. 10, Apr 2015. (Open Access).| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | MONNERAT, R.; PEREIRA, E.; TELES, B.; MARTINS, E.; PRACA, L.; QUEIROZ, P.; SOBERON, M.; BRAVO, A.; RAMOS, F.; SOARES, C. M. Synergistic activity of Bacillus thuringiensis toxins against Simulium spp. larvae. Journal of Invertebrate Pathology, v .121, p.70-73, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | MARTINS E. S.; PONTES, R. G. M. S. de; QUEIROZ P. R.; DUMAS, V. F.; BRAZ, S. V.; AGUIAR, R. W. DE S.; GOMES, A. C. M. M.; SANCHES, J.; BRAVO, A.; RIBEIRO, B. M. Midgut GPI-anchored proteins with alkaline phosphatase activity from the cotton boll weevil (Anthonomus grandis) are putative receptors for the Cry1B protein of Bacillus thuringiensis. Insect Biochemistry and Molecular Biology, v. 40, p. 138-145, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | GÓMEZ, I.; SÁNCHEZ, J.; LIMA, C.; MARTINS, E.; ROSALES-JUÁREZ, A.; AGUILAR-MEDEL, S.; ABAD, A.; DONG, H.; PONTES, R. G. M. S. de; PEÑA, G.; ZHANG, J.; NELSON, M.; WU, G.; BRAVO, A.; SOBERÓN, M. Enhancement of Bacillus thuringiensis Cry1Ab and Cry1Fa toxicity to Spodoptera frugiperda by domain III mutations indicates there are two limiting steps in toxicity as defined by receptor binding and protein stability Applied and Environmental Microbiology, v. 84, n. 20, article e01393-18, 2018. Na publicação: Rose Monnerat.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MARTINS, E. S.; RIBEIRO, B. M.; QUEIROZ, P. R.; DUMAS, V. F.; BRAZ, S. V.; AGUIAR, R. W. S.; GOMES, A. C. M. M.; SÁNCHEZ, J.; BRAVO, A.; PONTES, R. G. M. S. de. Primeiro relato de identificação de receptor para toxinas de Bacillus thuringiensis EM Anthonomus grandis. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 085. p. 126| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 23 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
02/01/2001 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAZIOLA, S. A.; ALESSI, E. S.; GUIMARAES, P. E. O.; DAMERVAL, C.; AZEVEDO, R. A. |
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Afiliação: |
PAULO EVARISTO DE O GUIMARAES, CNPMS. |
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Título: |
Quality protein maize: a biochemical study of enzymes involved in lysine metabolism. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington, v. 47, n. 3, p. 1268-1275, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
Quality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have intensified the effect of the opaque-2 mutation on lysine ketoglutarate reductase-saccharopine dehydrogenase. These alterations lead to an increase in soluble lysine in the endosperm of the QPM varieties when compared to the opaque-2 and wild type. MenosQuality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have i... Mostrar Tudo |
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Palavras-Chave: |
Opaco-2; Opaque-2; Protein; QPM; Quality. |
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Thesagro: |
Enzima; Lisina; Milho; Proteína; Qualidade; Zea Mays. |
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Thesaurus NAL: |
enzymes; lysine. |
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Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02601naa a2200325 a 4500
001 1485183
005 2018-06-07
008 1999 bl uuuu u00u1 u #d
100 1 $aGAZIOLA, S. A.
245 $aQuality protein maize$ba biochemical study of enzymes involved in lysine metabolism.$h[electronic resource]
260 $c1999
520 $aQuality protein maize (QPM) varieties have been produced by the introduction of opaque-2 modifier genes. Two QPM varieties, BR451, and BR473, a wild type and an opaque-2 variety, have beeen used to study key enzymes controlling lysine metabolism in the endosperm during development. Aspartate kinase and homoserine dehydrogenase enzymes. which are involved in lysine and threonine biosynthesis, respectively, exhibited identical activity patterns during endosperm development, with a maximum specific activity at 16 days after pollination. The QPM varieties exhibited higher levels of aspartate kinase activity in the endosperm, suggesting an increased rate of lysine biosynthesis when compared to the opaque-2 and wild-type genotypes. Similar results were observed for the lysine ketoglutarate reductase and saccharopine dehydrogenase enzymes, which form a single bifunctional polypetide involved in endosperm lysine degradation. Both enzyme activity were strongly reduced in the opaque-2 maize variety when compared to the wild-type maize, whereas the QPM varieties exhibited even lower levels of lysine ketoglutarate reductase-saccharopine dehydrogenase activities when compared to the opaque-2 variety. The developmental pattern of ensyme activity showed a different profile when compared to the enzymes involved in lysine biosynthesis, with activity being detected only 12-16 days after pollination (DAP) and maximum activities ~24 DAP. These results also suggest that the modifier genes have intensified the effect of the opaque-2 mutation on lysine ketoglutarate reductase-saccharopine dehydrogenase. These alterations lead to an increase in soluble lysine in the endosperm of the QPM varieties when compared to the opaque-2 and wild type.
650 $aenzymes
650 $alysine
650 $aEnzima
650 $aLisina
650 $aMilho
650 $aProteína
650 $aQualidade
650 $aZea Mays
653 $aOpaco-2
653 $aOpaque-2
653 $aProtein
653 $aQPM
653 $aQuality
700 1 $aALESSI, E. S.
700 1 $aGUIMARAES, P. E. O.
700 1 $aDAMERVAL, C.
700 1 $aAZEVEDO, R. A.
773 $tJournal of Agricultural and Food Chemistry, Washington$gv. 47, n. 3, p. 1268-1275, 1999.
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