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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
01/02/2016 |
Data da última atualização: |
01/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAULA, C. M. P. de; LIMA, D. C.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
Afiliação: |
Cristina Maria Pinto de Paula, UFLA, MG; Dayane Cristina Lima, UFLA, MG; FAUSTO DE SOUZA SOBRINHO, CNPGL; Vânia Helena Techio, UFLA, MG. |
Título: |
Frequency of the RAD51 (radiation sensitive 51) recombinase in species and hybrids of Brachiaria and relationship with genetic variability. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Australian Journal of Crop Science, v. 9, n. 12, p. 1271-1277, 2015. |
Idioma: |
Inglês |
Conteúdo: |
Abstract : This study identified and compared the frequency of homology and recombination hotspots in sexual diploid and tetraploid species, apomictic tetraploids and hybrids of Brachiaria, by immunolocalization of the radiation sensitive 51 (RAD51). Roots were fixed in 4% paraformaldehyde. We applied primary antibody (Rat anti-Arabidopsis thaliana RAD51) and detected with a secondary antibody (Goat anti-rat IgG FITC). The analysis of this protein showed diffuse signals at leptotene. In zygotene, signals of RAD51 were identified in unpaired chromosomes or partially paired and adjacent signals of RAD51 in paired chromosomes, confirming its close involvement with the search for chromosomal homology. During pachytene, RAD51 signals were located between chromosome axes in recombination hotspots. The mean number of recombination signals was greater in apomictic species of Brachiaria (B. decumbens, 85.80 and B. brizantha, 83.87), this higher frequency of signals can be related to the mode of reproduction of the species. The artificially tetraploidized B. ruziziensis showed a mean number of RAD51 signals similar the diploid B. ruziziensis, as a result of the polyploidization. The hybrids presented an overlap of the mean number of RAD51 signals compared to diploid and tetraploid of B. ruziziensis. This non-additivity of genomes is possibly an adaptation to genomic stress caused by hybridization and polyploidy. |
Palavras-Chave: |
Crossover; Homology; Immunolocalization; Recombination hotspots. |
Thesaurus Nal: |
meiosis. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02129naa a2200217 a 4500 001 2035705 005 2024-02-01 008 2015 bl uuuu u00u1 u #d 100 1 $aPAULA, C. M. P. de 245 $aFrequency of the RAD51 (radiation sensitive 51) recombinase in species and hybrids of Brachiaria and relationship with genetic variability.$h[electronic resource] 260 $c2015 520 $aAbstract : This study identified and compared the frequency of homology and recombination hotspots in sexual diploid and tetraploid species, apomictic tetraploids and hybrids of Brachiaria, by immunolocalization of the radiation sensitive 51 (RAD51). Roots were fixed in 4% paraformaldehyde. We applied primary antibody (Rat anti-Arabidopsis thaliana RAD51) and detected with a secondary antibody (Goat anti-rat IgG FITC). The analysis of this protein showed diffuse signals at leptotene. In zygotene, signals of RAD51 were identified in unpaired chromosomes or partially paired and adjacent signals of RAD51 in paired chromosomes, confirming its close involvement with the search for chromosomal homology. During pachytene, RAD51 signals were located between chromosome axes in recombination hotspots. The mean number of recombination signals was greater in apomictic species of Brachiaria (B. decumbens, 85.80 and B. brizantha, 83.87), this higher frequency of signals can be related to the mode of reproduction of the species. The artificially tetraploidized B. ruziziensis showed a mean number of RAD51 signals similar the diploid B. ruziziensis, as a result of the polyploidization. The hybrids presented an overlap of the mean number of RAD51 signals compared to diploid and tetraploid of B. ruziziensis. This non-additivity of genomes is possibly an adaptation to genomic stress caused by hybridization and polyploidy. 650 $ameiosis 653 $aCrossover 653 $aHomology 653 $aImmunolocalization 653 $aRecombination hotspots 700 1 $aLIMA, D. C. 700 1 $aSOUZA SOBRINHO, F. de 700 1 $aTECHIO, V. H. 773 $tAustralian Journal of Crop Science$gv. 9, n. 12, p. 1271-1277, 2015.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
25/04/2012 |
Data da última atualização: |
09/05/2012 |
Autoria: |
VIEIRA, J. C.; ALBUQUERQUE, M. M. de; LEMOS, J. WL V.; OLIVEIRA, F. G. de. |
Afiliação: |
JOSE CAVALCANTE VIEIRA, CPATC; MARCONDES MAURICIO DE ALBUQUERQUE, CPATC; JOSE WILLIAM VERAS LEMOS, CPATC; FRANCISCO FERREIRA DE OLIVEIRA, EPEAL. |
Título: |
Feijão carioca: reação a doenças em Alagoas. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
Aracaju: Embrapa Tabuleiros Costeiros, 2000. |
Série: |
(Embrapa Tabuleiros Costeiros. Comunicado Técnico, 35). |
Idioma: |
Português |
Palavras-Chave: |
Melhoramento genético. |
Thesagro: |
Doença de Planta; Feijão. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/58265/1/Feijaocarioca0001.pdf
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Marc: |
LEADER 00575nam a2200181 a 4500 001 1923022 005 2012-05-09 008 2000 bl uuuu u0uu1 u #d 100 1 $aVIEIRA, J. C. 245 $aFeijão carioca$breação a doenças em Alagoas.$h[electronic resource] 260 $aAracaju: Embrapa Tabuleiros Costeiros$c2000 490 $a(Embrapa Tabuleiros Costeiros. Comunicado Técnico, 35). 650 $aDoença de Planta 650 $aFeijão 653 $aMelhoramento genético 700 1 $aALBUQUERQUE, M. M. de 700 1 $aLEMOS, J. WL V. 700 1 $aOLIVEIRA, F. G. de
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