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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/01/2009 |
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Data da última atualização: |
10/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, T. S.; VASCONCELOS, E. A. R.; ROCHA, T. L.; SA, M. F. G. de. |
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Afiliação: |
TIAGO SIQUEIRA SILVA; ÉRICO AUGUSTO ROSAS DE VASCONCELOS; THALES LIMA ROCHA, CENARGEN; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
Chitinolitic activity in proteic extracts of Bacillus thuringiensis toxic to boll weevil (Anthonomus grandis). |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Inglês |
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Thesagro: |
Anthonomus Grandis; Bacillus Thuringiensis; Toxina. |
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Thesaurus Nal: |
chitinase. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00677nam a2200193 a 4500
001 1190448
005 2024-05-10
008 2008 bl uuuu u00u1 u #d
100 1 $aSILVA, T. S.
245 $aChitinolitic activity in proteic extracts of Bacillus thuringiensis toxic to boll weevil (Anthonomus grandis).
260 $aIn: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq$c2008
300 $c1 CD-ROM.
650 $achitinase
650 $aAnthonomus Grandis
650 $aBacillus Thuringiensis
650 $aToxina
700 1 $aVASCONCELOS, E. A. R.
700 1 $aROCHA, T. L.
700 1 $aSA, M. F. G. de
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
13/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
WELLER, M. M.; ALBINO, R. L.; MARCONDES, M. I.; SILVA, W.; DANIELS, K. M.; CAMPOS, M. M.; DUARTE, M. S.; MESCOUTO, M. L.; SILVA, F. F.; GUIMARÃES, S. E. F. |
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Afiliação: |
M. M. WELLER, UFV; R. L. ALBINO, UFV; M. I. MARCONDES, UFV; W. SILVA, UFV; K. M. DANIELS, Virginia Polytechnic Institute and State University, Blacksburg; MARIANA MAGALHAES CAMPOS, CNPGL; M. S. DUARTE, UFV; M. L. MESCOUTO, UFV; F. F. SILVA, UFV; S. E. F. GUIMARÃES, UFV. |
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Título: |
Effects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 99, n. 12, p. 9962-9973, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated. MenosAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers,... Mostrar Tudo |
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Palavras-Chave: |
Dairy heifer; Mammary lipogenesis. |
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Thesaurus NAL: |
nutrigenomics. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03014naa a2200265 a 4500
001 2063812
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aWELLER, M. M.
245 $aEffects of nutrient intake level on mammary parenchyma growth and gene expression in crossbred (Holstein × Gyr) prepubertal heifers.$h[electronic resource]
260 $c2016
520 $aAbstract This study investigated the effects of increased nutrient intake levels on prepubertal mammary parenchyma development in crossbreed (Holstein × Gyr) dairy heifers. Eighteen heifers age 3 to 4 mo were fed 1 of 3 nutrient intake levels (n=6 per treatment) designed to sustain an average daily gain of 0.0kg/d (maintenance, MA), 0.5kg/d (low gain, LG), or 1.0kg/d (high gain, HG). Serum blood samples collected on d 42 and 84 after a 12-h fast were analyzed for triglycerides, leptin, insulin, and insulin-like growth factor 1 (IGF-1). Liver and mammary parenchyma were biopsied on d 42 and harvested on d 84 for gene expression analysis. Parenchyma samples were also used for biochemical and histological analysis. Mammary parenchyma weight was lower in HG than in MA or LG heifers, but mammary extraparenchymal fat was greater in HG heifers than in other groups. Heifers fed the HG diet had a greater fraction of ether extract in their parenchyma than the others and a smaller fraction of crude protein in their parenchyma than MA heifers. Moreover, the HG and LG heifers had greater body fat mass than MA heifers. Nutrient intake level had no effect on the number of intraparenchymal adipocytes. Heifers fed the HG diet had greater serum IGF-1 than the others, and serum insulin was lower in the MA than the HG or LG heifers. Liver GHR, IGF1, and IGFBP3 mRNA expression was higher, but IGFBP2 mRNA was lower in HG heifers than in others. The parenchyma mRNA expression of lipogenic markers, such as CD36, ACCA, FASN, and ADIPOR1, was upregulated by nutrient intake level. Significant nutrient intake × time interactions for lipogenic genes during the experimental period indicated variable gene expression depending on the time point of prepubertal mammary gland development. Overall, our data suggest that enhancing nutrient intake increased body fat accumulation and lipogenesis in the mammary gland to the detriment of parenchyma growth. Moreover, increased lipogenesis in the parenchyma of HG heifers may indicate that fat accumulation occurred because of adipocyte hypertrophy and not differences in adipogenesis. The implications of these results for milk yield needs to be elucidated.
650 $anutrigenomics
653 $aDairy heifer
653 $aMammary lipogenesis
700 1 $aALBINO, R. L.
700 1 $aMARCONDES, M. I.
700 1 $aSILVA, W.
700 1 $aDANIELS, K. M.
700 1 $aCAMPOS, M. M.
700 1 $aDUARTE, M. S.
700 1 $aMESCOUTO, M. L.
700 1 $aSILVA, F. F.
700 1 $aGUIMARÃES, S. E. F.
773 $tJournal of Dairy Science$gv. 99, n. 12, p. 9962-9973, 2016.
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