Data/hora: 23/06/2024 01:20:41

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 18/10/1994
Data da última atualização: | 14/01/2020
Autoria: | GILBERTSON, R. L.; FARIA, J. C.; AHLQUIST, P.; MAXWELL, D. P.
Afiliação: | ROBERT L. GILBERTSON; JOSIAS CORREA DE FARIA, CNPAF; PAUL AHLQUIST; DOUGLAS P. MAXWELL.
Título: | Genetic diversity in geminiviruses causing bean golden mosaic disease: the nucleotide sequence of the infectious cloned DNA components of a brazilian isolate of bean golden mosaic geminivirus.
Ano de publicação: | 1993
Fonte/Imprenta: | Phytopathology, St. Paul, v. 83, n. 7, p. 709-715, July 1993.
Idioma: | Inglês
Conteúdo: | The complete nucleotide (nt) sequence of a Brazilian isolate of bean golden mosaic geminivirus (BGMV-BZ) was determined from the infectious cloned DNA components. The genome of BGMV-BZ was divided between two DNA components, DNA-A (2,617 nt) and DNA-B (2,580 nt), which had little sequence identity except for a 181-nt common region present in both molecules. The genomic organization of BGMV-BZ was similar to that of other bipartite whitefly-transmitted geminiviruses, with six open reading frames (ORFs) encoding putative proteins >10 kDa, four on DNA-A (one in the viral [+] and three in the viral complementary sense [–]) and two on DNA-B (one in the viral and one in the viral complementary sense). All six BGMV-BZ ORFs were analogous to corresponding ORFs in other whitefly-transmitted geminiviruses. The homology between BGMV-BZ sequences and those of a BGMV isolate from Puerto Rico (PR) were relatively low. BGMV-BZ sequences were no more closely related to BGMV-PR than to other distinct geminiviruses from South America, such as tomato golden mosaic geminivirus. Pseudo-recombinants made by mixing the infectious cloned DNAs of BGMV-BZ and a BGMV isolate from Guatemala (GA), which was very similar to BGMV-PR, were not infectious. These results support previous observations of differences in sap-transmissibility and bean genotype reactions between BGMV isolates and indicate that at least two distinct types of BGMV exist, designated type I and II.
Palavras-Chave: | Geminivirus.
Thesagro: | DNA; Doença; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Vírus.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (6676-1) - UPC - AP - PP - 1993 -  - 1993 - DIS