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| 6. |  | AGUIAR-PERECIN, M. L. R.; MONDIN, M.; SANTOS-SEREJO, J. A. dos; VIDA, A. C. F.; MOLINA, S. C. M. Analysis of karyotype variability of tropical maize inbred lines and hybrids using FISH of tandemly repeated DNA sequences to identify somatic chromosomes. In: Chomosome Research, Netherlands, v. 15, n. 2, p. 41 jul. 2007. Supplement. P.088 Edição do 16 INTERNATIONAL CHROMOSOME CONFERENCE, Abstracts...Amsterdam, ago. 2007. Editado por Hans de Jong, Hans Tanke and Paul Fransz.
DOI: 10.1007/s10577
ISSN 0967-3849 em papel| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 8 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
05/11/2014 |
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Data da última atualização: |
26/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
MONDIN, M.; SEREJO, J. A. dos S.; BERTÃO, M. R.; LABORDA, P.; PIZZAIA, D.; AGUIAR-PERECIN, M. L. R. |
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Afiliação: |
MATEUS MONDIN, ESALQ; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; MÔNICA R. BERTÃO, ESALQ; PRIANDA LABORDA, UNICAMP; DANIEL PIZZAIA, ESALQ; MARGARIDA L. R. AGUIAR-PERECIN, ESALQ. |
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Título: |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Frontiers in Plant Science, October, v. 5, 2014. |
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ISSN: |
1664-462X |
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Idioma: |
Inglês |
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Conteúdo: |
Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA. MenosMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes.... Mostrar Tudo |
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Palavras-Chave: |
Heterochromatic knobs. |
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Thesagro: |
Milho. |
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Thesaurus NAL: |
Centromeres; Chromosomes; Corn; Satellite DNA. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aMONDIN, M.
245 $aKaryotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line.$h[electronic resource]
260 $c2014
520 $aMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.
650 $aCentromeres
650 $aChromosomes
650 $aCorn
650 $aSatellite DNA
650 $aMilho
653 $aHeterochromatic knobs
700 1 $aSEREJO, J. A. dos S.
700 1 $aBERTÃO, M. R.
700 1 $aLABORDA, P.
700 1 $aPIZZAIA, D.
700 1 $aAGUIAR-PERECIN, M. L. R.
773 $tFrontiers in Plant Science, October$gv. 5, 2014.
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