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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/02/2020 |
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Data da última atualização: |
17/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, R. J. S.; ALVES, R. M.; GRAMACHO, K. P.; MARCELLINO, L. H.; MICHELI, F. |
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Afiliação: |
Raner José Santana Silva, UESC; RAFAEL MOYSES ALVES, CPATU; Karina Peres Gramacho, CEPLAC/CEPEC; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, UESC / CIRAD. |
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Título: |
Involvement of structurally distinct cupuassu chitinases and osmotin in plant resistance to the fungus Moniliophthora perniciosa. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 148, p. 142-151, Mar. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The cupuassu tree (Theobroma grandiflorum) is a crop of great economic importance to Brazil, mainly for its pulp and seeds, which are used in food industry. However, cupuassu fruit production is threatened by witches' broom disease caused by the fungus Moniliophthora perniciosa. As elements of its defense mechanisms, the plant can produce and accumulate pathogenesis-related (PR) proteins such as chitinases and osmotins. Here, we identified three cupuassu PR proteins (TgPR3, TgPR5 and TgPR8) from cupuassu-M. perniciosa interaction RNA-seq data. TgPR3 and TgPR8 corresponded to chitinases, and TgPR5 to osmotin; they are phylogenetically related to cacao and to Arabidopsis PR sequences involved in biotic and abiotic stress. The TgPR proteins' tridimensional structure was obtained through homology modeling, and molecular docking with chitin and chitosan showed that the TgPR proteins can interact with both cell wall molecules and presented a higher affinity for chitosan. TgPR gene expression was analyzed by RT-qPCR on resistant and susceptible cupuassu genotypes infected by M. perniciosa at 8, 24, 48 and 72 h after infection (hai). The TgPR genes showed higher expression in resistant plants compared to the susceptible ones, mainly for TgPR5 at 8 and 24 hai, while the expression was lower in the susceptible cupuassu plants. To our knowledge, this is the first in silico and in vitro reports of cupuassu PR protein. The data suggested that TgPRs could be involved in recognizing mechanisms of the plant's innate immune system through chitin receptors. Our results also suggest a putative role of chitinase/chitosanase for the TgPR5/osmotin. MenosThe cupuassu tree (Theobroma grandiflorum) is a crop of great economic importance to Brazil, mainly for its pulp and seeds, which are used in food industry. However, cupuassu fruit production is threatened by witches' broom disease caused by the fungus Moniliophthora perniciosa. As elements of its defense mechanisms, the plant can produce and accumulate pathogenesis-related (PR) proteins such as chitinases and osmotins. Here, we identified three cupuassu PR proteins (TgPR3, TgPR5 and TgPR8) from cupuassu-M. perniciosa interaction RNA-seq data. TgPR3 and TgPR8 corresponded to chitinases, and TgPR5 to osmotin; they are phylogenetically related to cacao and to Arabidopsis PR sequences involved in biotic and abiotic stress. The TgPR proteins' tridimensional structure was obtained through homology modeling, and molecular docking with chitin and chitosan showed that the TgPR proteins can interact with both cell wall molecules and presented a higher affinity for chitosan. TgPR gene expression was analyzed by RT-qPCR on resistant and susceptible cupuassu genotypes infected by M. perniciosa at 8, 24, 48 and 72 h after infection (hai). The TgPR genes showed higher expression in resistant plants compared to the susceptible ones, mainly for TgPR5 at 8 and 24 hai, while the expression was lower in the susceptible cupuassu plants. To our knowledge, this is the first in silico and in vitro reports of cupuassu PR protein. The data suggested that TgPRs could be involved in recognizing mechan... Mostrar Tudo |
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Thesagro: |
Cupuaçu; Doença de Planta; Fungo; Theobroma Grandiflorum; Vassoura de Bruxa. |
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Thesaurus Nal: |
Chitin; Chitosan; Gene expression; Pathogenesis-related proteins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02500naa a2200277 a 4500
001 2120606
005 2020-04-17
008 2020 bl uuuu u00u1 u #d
100 1 $aSILVA, R. J. S.
245 $aInvolvement of structurally distinct cupuassu chitinases and osmotin in plant resistance to the fungus Moniliophthora perniciosa.$h[electronic resource]
260 $c2020
520 $aThe cupuassu tree (Theobroma grandiflorum) is a crop of great economic importance to Brazil, mainly for its pulp and seeds, which are used in food industry. However, cupuassu fruit production is threatened by witches' broom disease caused by the fungus Moniliophthora perniciosa. As elements of its defense mechanisms, the plant can produce and accumulate pathogenesis-related (PR) proteins such as chitinases and osmotins. Here, we identified three cupuassu PR proteins (TgPR3, TgPR5 and TgPR8) from cupuassu-M. perniciosa interaction RNA-seq data. TgPR3 and TgPR8 corresponded to chitinases, and TgPR5 to osmotin; they are phylogenetically related to cacao and to Arabidopsis PR sequences involved in biotic and abiotic stress. The TgPR proteins' tridimensional structure was obtained through homology modeling, and molecular docking with chitin and chitosan showed that the TgPR proteins can interact with both cell wall molecules and presented a higher affinity for chitosan. TgPR gene expression was analyzed by RT-qPCR on resistant and susceptible cupuassu genotypes infected by M. perniciosa at 8, 24, 48 and 72 h after infection (hai). The TgPR genes showed higher expression in resistant plants compared to the susceptible ones, mainly for TgPR5 at 8 and 24 hai, while the expression was lower in the susceptible cupuassu plants. To our knowledge, this is the first in silico and in vitro reports of cupuassu PR protein. The data suggested that TgPRs could be involved in recognizing mechanisms of the plant's innate immune system through chitin receptors. Our results also suggest a putative role of chitinase/chitosanase for the TgPR5/osmotin.
650 $aChitin
650 $aChitosan
650 $aGene expression
650 $aPathogenesis-related proteins
650 $aCupuaçu
650 $aDoença de Planta
650 $aFungo
650 $aTheobroma Grandiflorum
650 $aVassoura de Bruxa
700 1 $aALVES, R. M.
700 1 $aGRAMACHO, K. P.
700 1 $aMARCELLINO, L. H.
700 1 $aMICHELI, F.
773 $tPlant Physiology and Biochemistry$gv. 148, p. 142-151, Mar. 2020.
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Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
18/09/2018 |
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Data da última atualização: |
18/09/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A. |
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Afiliação: |
Karina S. de Oliveira, UFSCAR; Andressa O. de Lima, UFSCAR; Juliana Afonso, UFSCAR; Alícia L. de Melo, UNIVASF; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. |
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Páginas: |
p. 26. |
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Série: |
(Embrapa Instrumentação. Documentos, 68). |
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ISSN: |
1518-7179 |
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Idioma: |
Português |
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Notas: |
Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva. |
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Conteúdo: |
A eficiência alimentar é uma característica moderadamente herdável, que por possuir uma mensuração onerosa e tardia, tem sua incorporação em programas de melhoramento animal dificultada. Estudos prévios de mapeamento identificaram o gene KCNJ7 7, que possui importante função sobre vias metabólicas relacionadas a eficiência alimentar, tais como a via de insulina, em uma região de QTL (quantitative trait loci) para maciez da carne. |
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Palavras-Chave: |
Sítios-alvo de fator de transcrição. |
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Thesagro: |
Bos Indicus; Gado Nelore; Melhoramento Genético Animal. |
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Thesaurus NAL: |
Animal breeding; Transcription factors. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/183211/1/VariantesRegiaoPromotoraGene.pdf
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Marc: |
LEADER 01533nam a2200277 a 4500
001 2095987
005 2018-09-18
008 2018 bl uuuu u00u1 u #d
022 $a1518-7179
100 1 $aOLIVEIRA, K. S. de
245 $aVariantes da região promotora do gene KCNJ11 em bovinos da raça Nelore.$h[electronic resource]
260 $aIn: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste$c2018
300 $ap. 26.
490 $a(Embrapa Instrumentação. Documentos, 68).
500 $aEditores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.
520 $aA eficiência alimentar é uma característica moderadamente herdável, que por possuir uma mensuração onerosa e tardia, tem sua incorporação em programas de melhoramento animal dificultada. Estudos prévios de mapeamento identificaram o gene KCNJ7 7, que possui importante função sobre vias metabólicas relacionadas a eficiência alimentar, tais como a via de insulina, em uma região de QTL (quantitative trait loci) para maciez da carne.
650 $aAnimal breeding
650 $aTranscription factors
650 $aBos Indicus
650 $aGado Nelore
650 $aMelhoramento Genético Animal
653 $aSítios-alvo de fator de transcrição
700 1 $aLIMA, A. O. de
700 1 $aAFONSO, J.
700 1 $aMELO, A. L. de
700 1 $aREGITANO, L. C. de A.
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Embrapa Pecuária Sudeste (CPPSE) |
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