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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/07/2019 |
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Data da última atualização: |
05/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, S. E. M.; GIMENES, M. A.; OLIVEIRA JUNIOR, J. O. L. de; COSTA, M. F.; GOMES, M. F. C.; VALENTE, S. E. S. |
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Afiliação: |
MARCOS APARECIDO GIMENES, Cenargen; JOSE OSCAR LUSTOSA DE O JUNIOR, CPAMN; M. F. COSTA, UFPI; M. F. C.GOMES, UESPI; S. E. S.VALENTE, UFPI. |
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Título: |
Consequences of accelerated aging for DNA integrity and seed germination of cowpea. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 3, article gmr18304, 2019. |
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DOI: |
10.4238/gmr18304 |
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Idioma: |
Inglês |
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Conteúdo: |
Deterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars. MenosDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processe... Mostrar Tudo |
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Palavras-Chave: |
Orthodox seeds; Seed bank. |
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Thesagro: |
Vigna Unguiculata. |
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Thesaurus Nal: |
Genetic resources. |
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Categoria do assunto: |
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G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/199456/1/gmr18304-consequences-accelerated-aging-dna-integrity-and-seed-germination-cowpea.pdf
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Marc: |
LEADER 02315naa a2200241 a 4500
001 2110566
005 2020-03-05
008 2019 bl uuuu u00u1 u #d
024 7 $a10.4238/gmr18304$2DOI
100 1 $aSANTOS, S. E. M.
245 $aConsequences of accelerated aging for DNA integrity and seed germination of cowpea.$h[electronic resource]
260 $c2019
520 $aDeterioration and loss of seed germination in the germplasm are related to the aging that occurs during the conservation process. The mechanisms related to the aging and death of seeds are not yet fully understood, but it is known that longevity can be affected by moisture content and temperature. We examined the effects of accelerated aging (A.A.) on the DNA of seeds of three different cultivars of cowpea (Vigna unguiculata) and compared the data to data from vigor and germination tests. The DNA of A.A. - seeds of BR 17 Gurgueia, BRS-Marataoã and BRS-Guariba cowpea cultivars were extracted and subjected to agarose gel electrophoresis. The increase in temperature, relative humidity and time used in A.A. culminated in a progressive and increasing degradation of seed DNA, which was expressed by continuous bands of DNA molecules with different lengths in the electrophoresis gel under all but the most extreme conditions (combinations 45°C/72 h and 45°C/96 h at 100% relative humidity) under which total disintegration of the DNA band was observed. This, in turn, was reflected in a progressive decrease in seed vigor. The combination of 35ºC/48 h was the most efficient for evaluating vigor in cowpea. The BR 17 Gurgueia and BRS-Guariba cultivars showed the highest values of vigor. A lack of DNA degradation in the laddering pattern on an electrophoresis gel is an indicator that DNA degradation and cell death in cowpea seeds did not occur by apoptosis but by non-programmed processes, such as Maillard reactions, lipid peroxidation and hydrolysis of sugars.
650 $aGenetic resources
650 $aVigna Unguiculata
653 $aOrthodox seeds
653 $aSeed bank
700 1 $aGIMENES, M. A.
700 1 $aOLIVEIRA JUNIOR, J. O. L. de
700 1 $aCOSTA, M. F.
700 1 $aGOMES, M. F. C.
700 1 $aVALENTE, S. E. S.
773 $tGenetics and Molecular Research$gv. 18, n. 3, article gmr18304, 2019.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
28/03/2001 |
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Data da última atualização: |
12/04/2017 |
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Autoria: |
SILVA, A. E. D. F.; DODE, M. A.; PORTO, J. A.; ABREU, U. G. P. |
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Afiliação: |
EMBRAPA Pecuaria Sudeste (Sao Carlos, SP). EMBRAPA Gado de Corte (Campo Grande, MS). EMBRAPA Pantanal (Corumba, MS). |
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Título: |
Estacionalidade na atividade sexual de machos bovinos Nelore e mesticos Fleckvieh e Chianina x Nelore: caracteristicas biometricas testiculares. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.26, n.10, p.1745-1750, out. 1991. |
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Idioma: |
Português |
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Conteúdo: |
Estudou-se o efeito das estacoes do ano, seca (junho a outubro de 1985) e chuvosa (janeiro a maio 1986), nas caracteristicas biometricas, perimetro escrotal e consistencia testicular, e peso vivo de dez touros, seis da raca Nelore e quatro meio-sangue Fleckvieh e Chianina x Nelore. O grupo genetico influenciou (P<0,001) todos os parametros estudados. O peso e perimetro escrotal foram maiores nos mesticos e a consistencia no Nelore. As epocas do ano afetaram significativamente (P<0,001) o peso vivo e o perimetro escrotal, maiores na epoca chuvosa, e, a consistencia testicular maior na epoca seca. A consistencia testicular correlacionou-se negativamente com o peso vivo, alem da temperatura e indice pluviometrico. Os machos Nelore, bem como os mesticos, sofreram a influencia da sazonalidade quanto as caracteristicas testiculares, devendo, portanto, apresentar maior atividade sexual no periodo de chuva, de maior disponibilidade alimentar nas condicoes do Brasil Central. |
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Palavras-Chave: |
Bovine; Consistencia testicular; Perimetro escrotal; Scrotal circumference; Testicular consistency. |
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Thesagro: |
Bovino; Reprodução. |
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Thesaurus NAL: |
reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01781naa a2200253 a 4500
001 1805983
005 2017-04-12
008 1991 bl --- 0-- u #d
100 1 $aSILVA, A. E. D. F.
245 $aEstacionalidade na atividade sexual de machos bovinos Nelore e mesticos Fleckvieh e Chianina x Nelore$bcaracteristicas biometricas testiculares.
260 $c1991
520 $aEstudou-se o efeito das estacoes do ano, seca (junho a outubro de 1985) e chuvosa (janeiro a maio 1986), nas caracteristicas biometricas, perimetro escrotal e consistencia testicular, e peso vivo de dez touros, seis da raca Nelore e quatro meio-sangue Fleckvieh e Chianina x Nelore. O grupo genetico influenciou (P<0,001) todos os parametros estudados. O peso e perimetro escrotal foram maiores nos mesticos e a consistencia no Nelore. As epocas do ano afetaram significativamente (P<0,001) o peso vivo e o perimetro escrotal, maiores na epoca chuvosa, e, a consistencia testicular maior na epoca seca. A consistencia testicular correlacionou-se negativamente com o peso vivo, alem da temperatura e indice pluviometrico. Os machos Nelore, bem como os mesticos, sofreram a influencia da sazonalidade quanto as caracteristicas testiculares, devendo, portanto, apresentar maior atividade sexual no periodo de chuva, de maior disponibilidade alimentar nas condicoes do Brasil Central.
650 $areproduction
650 $aBovino
650 $aReprodução
653 $aBovine
653 $aConsistencia testicular
653 $aPerimetro escrotal
653 $aScrotal circumference
653 $aTesticular consistency
700 1 $aDODE, M. A.
700 1 $aPORTO, J. A.
700 1 $aABREU, U. G. P.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.26, n.10, p.1745-1750, out. 1991.
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