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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/09/2000 |
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Data da última atualização: |
08/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VALICENTE, F. H.; BARRETO, M. R.; VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F. de; PAIVA, E. |
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Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS; MARIA JOSE VILACA DE VASCONCELOS, CNPMS. |
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Título: |
Identificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Anais da Sociedade Entomológica do Brasil, Jaboticabal, v. 29, n. 1, p. 147-153, 2000. |
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Idioma: |
Português |
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Conteúdo: |
Fall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains. |
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Palavras-Chave: |
Cry1; Lagarta do cartucho. |
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Thesagro: |
Controle Biológico; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control; Insecta. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/45185/1/Identificacao-atraves.pdf
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Marc: |
LEADER 02030naa a2200241 a 4500
001 1484159
005 2018-06-08
008 2000 bl uuuu u00u1 u #d
100 1 $aVALICENTE, F. H.
245 $aIdentificação através de PCR dos genes Cryl de cepas de Bacillus thuringiensis Berliner eficientes contra a lagarta do cartucho, Spodoptera frugiperda (J.E. Smith) (Lepidoptera$bNoctuidae).$h[electronic resource]
260 $c2000
520 $aFall armyworm, Spodoptera frugiperda (J.E. Smith), is the most important maize insect pest in Brazil and its damage can reduce yield up to 34%. The objective of this work was to utilize the PCR technique to identify Bacillus thuringiensis (B.t) strains that control S. frugiperda in maize crops. Biossays showed that 16 strains were very effective against S. frugiperda, 15 of them collected from soil samples and one (T09) obtained at the Institute Pasteur. The DNA of these strains were probed with cryl general primers and their proteins were analyzed by SDS-PAGE eletrophoresis. All strains present positive results for cryl genes. To further characterize these strains, specific cryl primers were empolyed. PCR technique showed that some strains harbour the same cryl genes. The only difference was the amplification of an unexpected fragment of approximately 160bp when a mixture of cry1B and cry1D specific primers was used. Analysis by phase contrast microscope showed that crystal proteins produced by these strains were all bipyramidal crystals. Also, eltrophoretic analysis of proteins by SDS-PAGE showed the same protein banding pattern for most of the strains.
650 $abiological control
650 $aInsecta
650 $aControle Biológico
650 $aSpodoptera Frugiperda
653 $aCry1
653 $aLagarta do cartucho
700 1 $aBARRETO, M. R.
700 1 $aVASCONCELOS, M. J. V. de
700 1 $aFIGUEIREDO, J. E. F. de
700 1 $aPAIVA, E.
773 $tAnais da Sociedade Entomológica do Brasil, Jaboticabal$gv. 29, n. 1, p. 147-153, 2000.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Meio Ambiente. |
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Data corrente: |
18/04/2013 |
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Data da última atualização: |
09/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BELLON, S.; SÁ, T. D. de A.; ABREU, L. S. de; OLLIVIER, G. |
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Afiliação: |
S. BELLON, INRA; TATIANA DEANE DE ABREU SA, CPATU; LUCIMAR SANTIAGO DE ABREU, CNPMA; G. OLLIVIER, INRA. |
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Título: |
Agroecological transitions in scientific research programs in Brazil and France. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: WORLD CONGRESS OF RURAL SOCIOLOGY - The New Rural World: From Crisis to Opportunities, 13., 2012, Lisboa. [Proceedings...]. Lisboa: International Rural Sociology Association, Instituto Superior de Agronomia (ISA), Instituto Superior de Ciências Sociais e Políticas (ISCSP), 2012. |
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Idioma: |
Inglês |
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Notas: |
n. 0622. |
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Palavras-Chave: |
Transição agroecológica. |
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Categoria do assunto: |
B Sociologia Rural |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1066861/1/AGROECOLOGICAL-TRANSITIONS.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/81668/1/RA-AbreuLS-XIII-WCRS-2012-WG76.pdf
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Marc: |
LEADER 00732nam a2200157 a 4500
001 2066861
005 2024-05-09
008 2012 bl uuuu u00u1 u #d
100 1 $aBELLON, S.
245 $aAgroecological transitions in scientific research programs in Brazil and France.$h[electronic resource]
260 $aIn: WORLD CONGRESS OF RURAL SOCIOLOGY - The New Rural World: From Crisis to Opportunities, 13., 2012, Lisboa. [Proceedings...]. Lisboa: International Rural Sociology Association, Instituto Superior de Agronomia (ISA), Instituto Superior de Ciências Sociais e Políticas (ISCSP)$c2012
500 $an. 0622.
653 $aTransição agroecológica
700 1 $aSÁ, T. D. de A.
700 1 $aABREU, L. S. de
700 1 $aOLLIVIER, G.
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Embrapa Amazônia Oriental (CPATU) |
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