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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus Nal: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
LEADER 02452naa a2200265 a 4500
001 2118709
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/02/2013 |
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Data da última atualização: |
19/03/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
COSTA, R. R.; ROCHA, A. S.; ALENCAR, A. P.; FONSECA JR. A. A.; ABI-ZAID, K. C. F.; SILVA, M. R.; BONAN, P. R. F.; SOARES FILHO, P.; SUFFYS, P. N. |
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Afiliação: |
ROSEANE MARIA RIBEIRO COSTA, FHEMIG; A. S. ROCHA, UFJF; A. P. ALENCAR, UFJF; A. A. FONSECA JR., UFJF; K. C. F. ABI-ZAID, FHEMIG; MARCIO ROBERTO SILVA, CNPGL; P. R. F. BONAN, FIOCRUZ; P. SOARES FILHO, FIOCRUZ; P. N. SUFFYS, LANAGRO. |
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Título: |
Perfil epidemiológico, clínico e de diagnostico de tuberculose em um hospital referência no estado de Minas Gerais, Brasil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Revista Médica de Minas Gerais, v. 22, supl. 2, p. S138, 2012. |
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Idioma: |
Português |
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Notas: |
Edição dos resumos do 3º Fórum Científico da Fundação Hospitalar do Estado de Minas Gerais, Juiz de Fora, 2012. |
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Thesagro: |
Tuberculose. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/949809/1/Perfil-epidemiologico-clinico-.pdf
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Marc: |
LEADER 00819nam a2200217 a 4500
001 1949809
005 2024-03-19
008 2012 bl uuuu u00u1 u #d
100 1 $aCOSTA, R. R.
245 $aPerfil epidemiológico, clínico e de diagnostico de tuberculose em um hospital referência no estado de Minas Gerais, Brasil.$h[electronic resource]
260 $aRevista Médica de Minas Gerais, v. 22, supl. 2, p. S138, 2012.$c2012
500 $aEdição dos resumos do 3º Fórum Científico da Fundação Hospitalar do Estado de Minas Gerais, Juiz de Fora, 2012.
650 $aTuberculose
700 1 $aROCHA, A. S.
700 1 $aALENCAR, A. P.
700 1 $aFONSECA JR. A. A.
700 1 $aABI-ZAID, K. C. F.
700 1 $aSILVA, M. R.
700 1 $aBONAN, P. R. F.
700 1 $aSOARES FILHO, P.
700 1 $aSUFFYS, P. N.
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Embrapa Gado de Leite (CNPGL) |
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