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| Registros recuperados : 127 | |
| 21. |  | CARVALHO, T. C.; RODRIGUES, C. M.; ABDELNUR, P. V.; RIBEIRO, J. A. de A.; CAMPANHA, R. B.; VAZ, B. G. Análise direta de açúcares em licores de bagaço de cana-de-açúcar por espectrometria de massas com Paper Spray Ionization (PSI). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 37., 2014, Natal. Abstracts... São Paulo: Sociedade Brasileira de Química, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 24. | | SOUZA, M. de A.; GUIMARÃES, M. B.; RODRIGUES NETO, J. C.; MENDONCA, S.; SIQUEIRA, F. G. de; ABDELNUR, P. V. Metabolômica de Schizophyllum commune cultivados em torta do caroço de algodão por espectrometria de massas. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 49-56 il.| Biblioteca(s): Embrapa Agroenergia. |
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| 25. | | MIRANDA, C. H. B.; CAVALCANTI, C. G. C.; CEREIJO. C. R.; ABDELNUR, P. V.; COSTA, P. P. K. G. Diversity of phytohormones within microalgae. In: SIMPÓSIO BRASIL-ALEMANHA, 9., 2019, Stuttgart, Alemanha. Integrating Systems for Sustainable Development - A Cross Continental Partnership. Book of programme and abstracts. Stuttgart: Baden-Württemberg Brazilian Center of the University of Tübingen, University of Hohenheim, Institute of Tropical Agricultural Sciences, Hans-Ruthenberg-Institute and Baden-Württemberg International, 2019. p. 58.| Biblioteca(s): Embrapa Agroenergia. |
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| 26. | | ABDELNUR, P. V.; RIBEIRO, J. A. de A.; IGLESIAS, A. H.; RODRIGUES, C. M.; LAVIOLA, B. G.; MENDONCA, S. Development of an ultra-fast method to detection of phorbol ester in Jatropha curcas using UHPLC-MS. In: PRIMER CONGRESO ARGENTINO DE ESPECTROMETRÍA DE MASA, 2012, Córdoba, Argentina. [S.l] Sociedad Argentina de Espectrometría de Masa, 2012.| Biblioteca(s): Embrapa Agroenergia. |
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| 27. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; RODRIGUES, C. M.; ABDELNUR, P. V. Otimização de métodos cromatográficos para separação de ácidos orgânicos, monossacarídeos, álcoois e açúcares fosfatados In: CONGRESSO BRASILEIRO DE QUÍMICA, 55., 2015, Goiânia. Recursos renováveis: inovação e tecnologia. Rio de Janeiro: ABQ, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 28. | | TEIXEIRA, T. S.; RODRIGUES NETO, J. C.; SILVA, E. A.; CONCEIÇÃO, A. A.; SIQUEIRA, F. G. de; ABDELNUR, P. V. Mass spectrometry imaging for fungal interaction analysis: Classic versus imprinting methods. Brazilian Journal of Analytical Chemistry, v. 10, n. 38, p. 71-78, 2023.| Biblioteca(s): Embrapa Agroenergia. |
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| 31. | | SILVA, L. C.; CARVALHO, T. C.; SILVA, I. P.; ABDELNUR, P. V.; LAVIOLA, B. G.; VAZ, B. G. Espectrometria de massas por probe electrospray ionization (PESI-MS) com polímero molecularmente impresso (MIP) para determinação de forbol em folhas de Jatropha curcas. In: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 19., CONGRESSO IBEROAMERICANO DE QUÍMICA ANALÍTICA, 7., 2018, Caldas Novas, GO. Livro de resumos. São Paulo: Metrohm, 2018. p. 354.| Biblioteca(s): Embrapa Agroenergia. |
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| 32. | | PEREIRA, R. C. L.; ABDELNUR, P. V.; TEIXEIRA, M. A. G.; RETORI, E. S.; ALMEIDA, M. B. B. de. ESI-MS and ESI-MS/MS as a toll to evaluate upgrading processes of bio-oils. In: ASMS CONFERENCE ON MASS SPECTROMETRY AND ALLIED TOPICS, 59., 2011, Denver, Colorado. [Proceedings...] Santa Fe: ASMS, 2011. Disponível em:. Acesso em: jun. 2011.| Biblioteca(s): Embrapa Agroenergia. |
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| 33. | | MONTEIRO, S.; RIBEIRO, J. A. de A.; ALMEIDA, E. S. DE.; NETO, B. A. D.; ABDELNUR, P. V. Identification of carotenoid isomers by mass spectrometry in crude and bleached palm oil. In: EURO FED LIPID CONGRESS, 15.; SYMPOSIUM OF THE NORDIC LIPIDFORUM, 29., 2017, Uppsala, Sweden. Oil, fats and lipids: new technologies and applications for a healthier life: book of abstracts. Frankfurt: Euro Fed Lipid, 2017. p. 53.| Biblioteca(s): Embrapa Agroenergia. |
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| 35. | | SOUTO, A. L.; RODRIGUES-NETO, J. C.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V. Identificação de marcadores químicos do amarelecimento fatal em Elaeis guineensis por UHPLC-MS e PCA. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 39., 2016, Goiânia, GO. Criar e empreender: anais ... São Paulo: SBQ, 2016. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 36. | | SILVA, C.; NEWMANN, B. C.; RIBEIRO, J. A. de A.; SOUTO, A. L.; ABDELNUR, P. V.; FERREIRA FILHO, E. X. Hydrothermal pretreatment of sugarcane bagasse enhances holocellulases production by Aspergillus foetidus. In: GORDON RESEARCH CONFERENCE - CELLULASES AND OTHER CARBOHYDRATE-ACTIVE ENZYMES, 2017, Andover, US. [Proceedings ...]. [S.l]: GRC, 2017. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 38. | | CAMPOS, C. G.; NASCIMENTO, I. F.; VERAS, H. C. T.; ALMEIDA, J. R. M. de; PARACHIN, N.; ABDELNUR, P. V. Desenvolvimento de protocolo para análise estatística de metabolômica de leveduras por ANOVA. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 33.| Biblioteca(s): Embrapa Agroenergia. |
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| 39. | | RODRIGUES NETO, J. C.; SILVA, M. E. R. da; COSTA, P. P. K. G.; RODRIGUES, C. M.; ABDELNUR, P. V. Desenvolvimento de protocolo analítico para folhas de dendê utilizando imagem química por MALDI-MS. In: WORKSHOP DA PÓS-GRADUAÇÃO EM QUÍMICA, 1., 2015, Goiânia, GO. [Resumos ...] Goiânia: UFG, 2015. WSPGQ-032| Biblioteca(s): Embrapa Agroenergia. |
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| 40. | | RICCI-SILVA, M. E.; NETO, J. C. R.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V. Desenvolvimento de protocolo analítico para raízes de dendê utilizando UPLC-ESI-Q-TOF-MS In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 84-85| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 127 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/03/2020 |
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Data da última atualização: |
27/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SILVA, C. de O. G.; TEIXEIRA, S. T.; RODRIGUES, K. B.; SOUZA, A. A.; MONCLARO, A. V.; MENDES, T. D.; RIBEIRO, J. A. de A.; SIQUEIRA, F. G. de; FAVARO, L. C. de L.; ABDELNUR, P. V. |
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Afiliação: |
Caio de Oliveira Gorgulho Silva; Tallyta Santos Teixeira, Universidade Federal de Tocantins; Kelly Barreto Rodrigues; Amanda Araujo Souza; Antonielle Vieira Monclaro; THAIS DEMARCHI MENDES, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE. |
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Título: |
Combination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Analytical Methods, n. 2, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Lytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products. MenosLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type... Mostrar Tudo |
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Palavras-Chave: |
Caracterização de LPMOs. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02626naa a2200241 a 4500
001 2121326
005 2020-04-27
008 2020 bl uuuu u00u1 u #d
100 1 $aSILVA, C. de O. G.
245 $aCombination of MALDI-TOF MS and UHPLC-ESI-MS for the characterization of lytic polysaccharide monooxygenase activity.$h[electronic resource]
260 $c2020
520 $aLytic polysaccharide monooxygenases (LPMOs) are redox enzymes of high biotechnological interest due to their capacity to degrade recalcitrant polysaccharides, such as cellulose, by an oxidative mechanism. The characterization of LPMOs is challenging since they generate a variety of catalytic products which include native oligosaccharides (non-oxidized) and oligosaccharides oxidized at the reducing end (C1 position), the non-reducing end (C4 position), or both ends, with different degrees of polymerization. Moreover, oxidized products exist in equilibrium with their hydrated forms, which further complicates their identification. The lack of commercial analytical standards for all these possible forms of oxidized oligosaccharides and the low concentration of these products make LPMO functional characterization dependent on advanced mass spectrometry techniques capable of identifying the profile of oxidized products. Here, a new approach for the characterization of cellulose-active LPMOs based on the combination of MALDI-TOF MS and hydrophilic interaction UHPLC-ESI-MS was proposed and optimized. The LPMO TrAA9A from Trichoderma reesei was used as a model enzyme to develop and test the MS methods. MALDI-TOF MS and UHPLC-ESI-MS methods were both capable of identifying putative C1, C4 and C1/C4 oxidized cello-oligosaccharides as well as their native counterparts generated after cellulose treatment with LPMO, which allows their utilization to characterize type I (C1-oxidizer), type II (C4-oxidizer) and type III (C1- and C4-oxidizer) LPMOs. Moreover, both methods were complementary since MALDI-TOF MS was capable of detecting oligosaccharides with higher degrees of polymerization (DP3?DP10), while UHPLC-ESI-MS allowed the evaluation of smaller oligosaccharides (DP1?DP5). The combined use of both methods offers a comprehensive description of LPMO catalytic products.
653 $aCaracterização de LPMOs
700 1 $aTEIXEIRA, S. T.
700 1 $aRODRIGUES, K. B.
700 1 $aSOUZA, A. A.
700 1 $aMONCLARO, A. V.
700 1 $aMENDES, T. D.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aSIQUEIRA, F. G. de
700 1 $aFAVARO, L. C. de L.
700 1 $aABDELNUR, P. V.
773 $tAnalytical Methods$gn. 2, 2020.
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