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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
01/06/2023 |
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Data da última atualização: |
01/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
SÍLVIA DE OLIVEIRA DORTA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, INSTITUTO AGRONÔMICO DE CAMPINAS; ODIMAR ZANUZO ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; JOÃO ROBERTO SPOTTI LOPES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MARCOS ANTONIO MACHADO, INSTITUTO AGRONÔMICO DE CAMPINAS; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Biotechnolog, v.68, May, p.60-70, 2023. |
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ISSN: |
0168-1656 |
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DOI: |
https://doi.org/10.1016/j.jbiotec.2023.04.007 |
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Idioma: |
Inglês |
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Conteúdo: |
The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. |
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Thesagro: |
Laranja; Melhoramento Vegetal. |
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Thesaurus Nal: |
Oranges. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02204naa a2200241 a 4500
001 2154159
005 2023-06-01
008 2023 bl uuuu u00u1 u #d
022 $a0168-1656
024 7 $ahttps://doi.org/10.1016/j.jbiotec.2023.04.007$2DOI
100 1 $aDORTA, S. DE O.
245 $aGenetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera$bLiviidae).$h[electronic resource]
260 $c2023
520 $aThe Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
650 $aOranges
650 $aLaranja
650 $aMelhoramento Vegetal
700 1 $aATTÍLIO, L. B.
700 1 $aZANARDI, O. Z.
700 1 $aLOPES, J. R. S.
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tJournal of Biotechnolog$gv.68, May, p.60-70, 2023.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 2. |  | ABARZUA, C.; PAVAN, C.; BASILE, D.; DANI, D.; SCOTTÁ, D.; BARBIERI, E.; BOZZA, E.; ANGHEBEN, I.; TAFFAREL, J. C.; SANTINI, L.; BOTTEGA, L.; BAVARESCO, L.; GARRIDO, L. da R.; MANFROI, L.; DALLÉ, M.; BOTTON, M.; DULLIUS, M. de V.; COPAT, M.; CRIPPA, P.; DULLIUS, P.; BOSCATTO, R.; BAO, R.; POSTAL, T. Manual de produção de uvas viníferas de alta qualidade. Bento Gonçalves, RS: União Brasileira de Vitivinicultura (UVIBRA), 2015. 46| Tipo: Autoria/Organização/Edição de Livros |
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