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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
29/11/2021 |
Data da última atualização: |
29/11/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, C. I.; COSTA, D. M. da; SANTOS, A. C. V.; BATATINHA, M. J. M.; SOUZA, F. V. D.; SOUZA, E. H. de; BOTURA, M. B.; ALVES, C. Q.; SOARES, T. L.; BRANDÃO, H. N. |
Afiliação: |
CAROLINE ISSLER RODRIGUES, UEFS; DIEGO MOTA DA COSTA, UEFS; ACIDÁLIA CARINE VIEIRA SANTOS, UFBA; MARIA JOSÉ MOREIRA BATATINHA, UFBA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; EVERTON HILO DE SOUZA, UFRB; MARIANA BORGES BOTURA, UEFS; CLAYTON QUEIROZ ALVES, UEFS; TALIANE LEILA SOARES, UEFS; HUGO NEVES BRANDÃO, UEFS. |
Título: |
Assessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Veterinary Parasitology, v.285, 2020. |
ISSN: |
1873-2550 |
DOI: |
10.1016/j.vetpar.2020.109219 |
Idioma: |
Inglês |
Conteúdo: |
Species of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. MenosSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to i... Mostrar Tudo |
Thesagro: |
Abacaxi. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02931naa a2200265 a 4500 001 2136781 005 2021-11-29 008 2020 bl uuuu u00u1 u #d 022 $a1873-2550 024 7 $a10.1016/j.vetpar.2020.109219$2DOI 100 1 $aRODRIGUES, C. I. 245 $aAssessment of in vitro anthelmintic activity and bio-guided chemical analysis of BRS Boyrá pineapple leaf extracts.$h[electronic resource] 260 $c2020 520 $aSpecies of the Bromeliaceae are known for their pharmacological actions, including anthelmintic effects. The aim of this study was to investigate the in vitro anthelmintic activity of extracts and fractions of BRS Boyrá pineapple leaf against the eggs and infective larvae of gastrointestinal nematodes (Trichostrongylidae) of goats and to identify the compounds involved in this activity. Crude methanol, hexane, dichloromethane, ethyl acetate and residual hydromethanol extracts were investigated by quantitative analysis of phenolic and flavonoid contents, antioxidant activity, anthelmintic activity against gastrointestinal nematodes of goats. The extracts were submitted to chromatographic methods for substance isolation and spectrometric techniques to identify their structures. The anthelmintic activity was performed by in vitro assays with eggs and larvae of nematodes obtained from naturally infected donor goats. All extracts contained phenolic (2.22-14.12 g of gallic acid equivalent per 100 g of dry extract) and flavonoid compounds (0.13-1.45 g of quercetin equivalent per 100 g of dry extract). Bio-guided fractionation of the BRS Boyrá pineapple leaves showed high antioxidant activity (EC50 for DPPH of 2.16-21.38 mg mL-1 and inhibition of co-oxidation of B-carotene of 36.40-74.86%) and anthelmintic activity (15.69-100% inhibition of egg hatching). The ethyl acetate extract exhibited greatest activity in all assays. Through chromatographic column analysis it was possible to isolate three substances: B-sitosterol and stigmasterol mixture in dichloromethane and hexane extracts, identified by NMR and p-coumaric acid in the ethyl acetate extract, identified by HPLC-DAD. The isolated p-coumaric acid exhibited high ovicidal effect against goat gastrointestinal nematodes (IC50: 0.12 mg mL-1) and can be considered the active substance of the ethyl acetate extract. This study revealed for the first time that the pineapple BRS Boyrá possesses inhibitory activity against gastrointestinal nematodes (Haemonchus spp., Oesophagostomum spp. and Trichostrongylus spp.), and that p-coumaric acid is an important bioactive. 650 $aAbacaxi 700 1 $aCOSTA, D. M. da 700 1 $aSANTOS, A. C. V. 700 1 $aBATATINHA, M. J. M. 700 1 $aSOUZA, F. V. D. 700 1 $aSOUZA, E. H. de 700 1 $aBOTURA, M. B. 700 1 $aALVES, C. Q. 700 1 $aSOARES, T. L. 700 1 $aBRANDÃO, H. N. 773 $tVeterinary Parasitology$gv.285, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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162. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | AVILA, A. C. de; DELLA VECCHIA, P. T.; LIN, M. T.; OLIVEIRA, L. O. B.; ARAUJO, J. P. Levantamento de viroses infectando melao e melancia na regiao do Submedio Sao Francisco. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 23., 1983, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: SOB, 1983. p.61.Biblioteca(s): Embrapa Hortaliças. |
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163. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ALBUQUERQUE, L. C.; FERNANDES, F. R.; ÁVILA, A. C. de; RIBEIRO, S. G.; INQUE-NAGATA, A. K. Molecular and biological characterization of a Tomato yellow vein streak virus isolate. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 5.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 3., 2007, Ouro Preto. Program e abstracts. Viçosa, MG: Universidade Federal de Viçosa, 2007. p. 93.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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164. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | GIORDANO, L. de B.; ARAGÃO, F. A. S.; INOUE-NAGATA, A. K.; AVILA, A. C. de; FONSECA, M. E. N. Molecular characterization of bipartite germinuviruses in Brazil and genetic basis of resistance in selected Lycopersicon esculentum lines. WORLD CONGRESS ON THE PROCESSING TOMATO, 5., ISHS SYMPOSIUM ON PROCESSING TOMATO, 8., 2002, Istanbul. [Abstracts...].Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Hortaliças. |
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166. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | RIBEIRO, S. G.; BEZERRA, I. C.; REZENDE, R. O.; LIMA, M. F.; RESENDE, L. V.; AVILA, A. C. de. New tomato geminiviruses in mixed infections in Brazil. IN: INTERNATIONAL WORKSHOP ON BEMISIA AND GEMINIVIRAL DISEASES, 2., 1998, San Juan, Puerto Rico. Program & abstracts... San Juan, 1998. p.P-63.Biblioteca(s): Embrapa Hortaliças. |
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167. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. A new tospovirus naturally infecting cucurbitaceae in Brazil. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.Biblioteca(s): Embrapa Hortaliças. |
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168. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | REIS, A.; QUEZADO-DUVAL, A. M.; INOUE-NAGATA, A. K.; ÁVILA, A. C. de; LOPES, C. A. Manejo de doenças em pimentas no Brasil. In: ENCONTRO NACIONAL DO AGRONEGÓCIO PIMENTAS (CAPSICUM spp.), 2.; MOSTRA NACIONAL DE PIMENTAS E PRODUTOS DERIVADOS, 2., 2006, Brasília, DF. Anais... Brasília, DF: Embrapa Hortaliças, 2006. 14 p. CD-ROM.Biblioteca(s): Embrapa Hortaliças. |
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169. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BEZERRA, I. C.; LIMA, M. F.; RIBEIRO, S. G.; GIORDANO, L. B.; ZERBINI, F. M.; AVILA, A. C. de. Occurrence of geminivirus in tomato-producing areas in submedio São Francisco. Fitopatologia Brasileira, Brasilia, v. 22, p. 331, ago. 1997. Suplemento.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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170. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CAMPOS, M. A.; PETERS, J. A.; MELO, P.; AVILA, A. C. de; TORRES, A. C.; MONTE, D. C. Obtencao de batata transgenica cv. Baronesa com resistencia a "Potato Virus Y". Fitopatologia Brasileira, Brasilia, DF, v. 22, p. 333, 1997. Suplemento. Resumo 589. Congresso Brasileiro de Fitopatologia, 30., 1997.Biblioteca(s): Embrapa Hortaliças. |
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172. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, M. S.; MARTINS, C. R.; BEZERRA, I. C.; AVILA, A. C. de; NAGATA, T.; RESENDE, R. O. Movement protein an additional parameter for tospovirus taxonomy. Virus Review & Research, Sao Paulo, v.4, p.155, Nov. 1999. Resumo. Suplemento, 1.Biblioteca(s): Embrapa Hortaliças. |
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174. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | AVILA, A. C. de; RESENDE, R. de O.; BEZERRA, I. C.; POZZER, L.; NAGATA, T.; KITAJIMA, E. W. The potential use of Polymerase Chain Reaction on the detection of tospovirus species. Fitopatologia Brasileira, Brasilia, v.20, p.301, ago. 1995. Suplemento. Resumo.Biblioteca(s): Embrapa Hortaliças. |
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176. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | CRUZ, C. C.; MELO, P. E. de; AVILA, A. C. de; FERREIRA, A. T.; TORRES, A. C. Resistance to PVY in potato trasngenic clones cultivar Achat. Genetics and Molecular Biology, Ribeirao Preto, v.21, n.3, p.214, Sept. 1998. Resumo. Suplemento.Biblioteca(s): Embrapa Hortaliças. |
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177. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | INOUE-NAGATA, A. K.; BARBOSA, F. D.; CAIXETA, C. V. D.; MORELLO, R. M. S. C.; ÁVILA, A. C. de. Viruses predominantly detected on tomatoes, sweetpepper and chilli pepper in the Federal District and Goiás. Fitopatologia Brasileira, Brasília, v. 29, p. S149-150, ago. 2004. Suplemento. Resumo 455. Trabalho apresentado no 37º Congresso Brasileiro de Fitopatologia, 2004.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Hortaliças. |
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178. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | POZZER, L.; NAGATA, T.; LIMA, M. I.; KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de. "Sapeca" an onion disease in the sub-medio Sao Francisco region, Brazil, is caused by a tospovirus with a serologically distinct nucleocapsid protein. Fitopatologia Brasileira, Brasilia, v.19, p.321, ago. 1994. Resumo. Suplemento.Biblioteca(s): Embrapa Hortaliças. |
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Registros recuperados : 257 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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