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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
19/06/2019 |
Data da última atualização: |
13/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BOTTA, D.; ARRUDA, R. P. de; WATANABE, Y. F.; BALIEIRO, J. C. de C.; ROMANELLO, N.; BARRETO, A. do N.; PANTOJA, M. H. de A.; GIRO, A.; CARVALHO, C. P. T. de; OLIVEIRA, A. de S.; GARCIA, A. R. |
Afiliação: |
Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE. |
Título: |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Andrologia, v. 51, n. 6, e13266, jul. 2019. |
DOI: |
10.1111/and.13266 |
Idioma: |
Inglês |
Conteúdo: |
Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. |
Palavras-Chave: |
Semen quality; Thermotolerance. |
Thesagro: |
Inseminação Artificial; Touro. |
Thesaurus Nal: |
Bulls; Heat stress; In vitro fertilization. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/198716/1/BOTTA-et-al-2019-Influence-of-Post-thawing-Thermal-Environment-on-Bovine-Sperm-Characteristics.pdf
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Marc: |
LEADER 02263naa a2200337 a 4500 001 2109972 005 2023-03-13 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1111/and.13266$2DOI 100 1 $aBOTTA, D. 245 $aInfluence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.$h[electronic resource] 260 $c2019 520 $aOur aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. 650 $aBulls 650 $aHeat stress 650 $aIn vitro fertilization 650 $aInseminação Artificial 650 $aTouro 653 $aSemen quality 653 $aThermotolerance 700 1 $aARRUDA, R. P. de 700 1 $aWATANABE, Y. F. 700 1 $aBALIEIRO, J. C. de C. 700 1 $aROMANELLO, N. 700 1 $aBARRETO, A. do N. 700 1 $aPANTOJA, M. H. de A. 700 1 $aGIRO, A. 700 1 $aCARVALHO, C. P. T. de 700 1 $aOLIVEIRA, A. de S. 700 1 $aGARCIA, A. R. 773 $tAndrologia$gv. 51, n. 6, e13266, jul. 2019.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
05/09/1997 |
Data da última atualização: |
05/09/1997 |
Autoria: |
FONSECA, M. E. de N.; BOITEUX, L. S. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Viroides: minusculos RNAs parasitas de plantas vasculares dotados de caracteristicas biologicas e estrututas unicas. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
Revisao Anual de Patologia de Plantas, Passo Fundo, v.5, p.387-425, 1997. |
Idioma: |
Português |
Conteúdo: |
Origem e evolucao dos viroides. Origem das doencas induzidas por viroides. Moderna taxonomia e classificacao de viroides. Localizacao subcelular. Patogenese. Purificacao de viroides. Deteccao de viroides. Clonagem e sequenciamento de viroides. Caracteristicas das doencas causadas por viroides. Controle de doencas causadas por viroides. Viroides e doencas causadas por viroides no Brasil. Perspectivas da pesquisa de viroides no Brasil. |
Palavras-Chave: |
Classificacao; Control; Controle; Deteccao; Disease; Plant; Viroides. |
Thesagro: |
Doença; Planta; Taxonomia; Vírus. |
Thesaurus NAL: |
classification; detection; taxonomy; viroids. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01265naa a2200313 a 4500 001 1758037 005 1997-09-05 008 1997 bl --- 0-- u #d 100 1 $aFONSECA, M. E. de N. 245 $aViroides$bminusculos RNAs parasitas de plantas vasculares dotados de caracteristicas biologicas e estrututas unicas. 260 $c1997 520 $aOrigem e evolucao dos viroides. Origem das doencas induzidas por viroides. Moderna taxonomia e classificacao de viroides. Localizacao subcelular. Patogenese. Purificacao de viroides. Deteccao de viroides. Clonagem e sequenciamento de viroides. Caracteristicas das doencas causadas por viroides. Controle de doencas causadas por viroides. Viroides e doencas causadas por viroides no Brasil. Perspectivas da pesquisa de viroides no Brasil. 650 $aclassification 650 $adetection 650 $ataxonomy 650 $aviroids 650 $aDoença 650 $aPlanta 650 $aTaxonomia 650 $aVírus 653 $aClassificacao 653 $aControl 653 $aControle 653 $aDeteccao 653 $aDisease 653 $aPlant 653 $aViroides 700 1 $aBOITEUX, L. S. 773 $tRevisao Anual de Patologia de Plantas, Passo Fundo$gv.5, p.387-425, 1997.
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