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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
05/09/1994 |
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Data da última atualização: |
05/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, R. S.; ROBLETO, E. A.; HANDELSMAN, J. |
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Afiliação: |
RICARDO SILVA ARAUJO, CNPAF; EDUARDO A. ROBLETO; JO HANDELSMAN. |
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Título: |
A hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Applied and Environmental Microbiology, v. 60, n. 5, p. 1430-1436, May 1994. |
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DOI: |
https://doi.org/10.1128/aem.60.5.1430-1436.1994 |
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Idioma: |
Inglês |
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Conteúdo: |
We isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003. MenosWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surfa... Mostrar Tudo |
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Palavras-Chave: |
Hydrophobic; Mutant; PTCA. |
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Thesagro: |
Bactéria; Mutante; Nodulação; Rhizobium. |
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Thesaurus Nal: |
nodulation; Rhizobium etli; rhizosphere. |
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Categoria do assunto: |
B Sociologia Rural |
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Marc: |
LEADER 02428naa a2200277 a 4500
001 1196158
005 2021-10-05
008 1994 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1128/aem.60.5.1430-1436.1994$2DOI
100 1 $aARAUJO, R. S.
245 $aA hydrophobic mutant of Rhizobium etli altered in nodulation competitiveness and growth in the rhizosphere.$h[electronic resource]
260 $c1994
520 $aWe isolated and characterized CE3003, a Tn5-induced mutant with altered colony morphology derived from Rhizobium etli CE3. CE3003 produced domed colonies and was highly hydrophobic as indicated by its ability to partition into hexadecane, whereas its parent produced flat colonies and was hydrophilic. On bean plants, CE3003 induced nodules and reduced acetylene. CE3003 and CE3 grew at similar rates when they were grown separately or together in culture medium or inoculated singly onto bean seeds. However, when they were mixed at a 1:1 ratio and applied to seeds, CE3003 achieved significantly lower populations than CE3 in the rhizosphere. Five days after coinoculation of CE3 and CE3003, the population of the mutant was less than 10% of the population of CE3 in the bean rhizosphere. To determine the nodulation competitiveness of the mutant, it was coinoculated with CE3 at various ratios at planting, and the ratio of the nodules occupied by each strain was determined 21 days later. A 17,000-fold excess of CE3003 in mixed inocula was required to obtain equal nodule occupancy by the two strains. A genomic library of strain CE3 was mobilized into CE3003, and we identified a cosmid, pRA3003, that restored the parental colony morphology and hydrophilicity to the mutant. Restoration of the parental colony morphology was accompanied by recovery of the ability to grow competitively in the rhizosphere and to compete for nodulation of beans. The data show an association between cell surface hydrophobicity, nodulation competitiveness, and competitive growth in the rhizosphere in mutant CE3003.
650 $anodulation
650 $aRhizobium etli
650 $arhizosphere
650 $aBactéria
650 $aMutante
650 $aNodulação
650 $aRhizobium
653 $aHydrophobic
653 $aMutant
653 $aPTCA
700 1 $aROBLETO, E. A.
700 1 $aHANDELSMAN, J.
773 $tApplied and Environmental Microbiology$gv. 60, n. 5, p. 1430-1436, May 1994.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/06/2023 |
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Data da última atualização: |
26/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CARVALHO, J. N. de; CARVALHO, P. A. de; PIO, R.; BARBOSA, M. A. G.; LEAO, P. C. de S. |
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Afiliação: |
JULLYANNA NAIR DE CARVALHO, UNIVERSIDADE FEDERAL DE LAVRAS; POLLYANNA APARECIDA DE CARVALHO, CENTRO DE TECNOLOGIA CANAVIEIRA; RAFAEL PIO, UNIVERSIDADE FEDERAL DE LAVRAS; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA; PATRICIA COELHO DE SOUZA LEAO, CPATSA. |
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Título: |
Multitrait selection in seedless grape hybrids in semiarid regions of Brazil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Crop Science, v. 63, n. 4, p. 2091-2102, 2023. |
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DOI: |
10.1002/csc2.20990 |
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Idioma: |
Inglês |
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Conteúdo: |
The absence of seeds has been one of the most valued traits in grapes (Vitis spp.) for fresh consumption. However, the simultaneous selection of superior genotypes for multiple traits is complex. The objective of this study was to select genotypes of superior seedless table grapes in semiarid regions of Brazil. Multiple traits were considered by using the factor analysis and genotype?ideotype best linear unbiased prediction (FAI-BLUP) selection index. A total of 110 grape progenies from 24 crosses were evaluated for traits of agronomic interest. Each hybrid was represented by a single vine plant, without experimental design or repetition. The mixed-model methodology was used to predict the breeding values. The FAI-BLUP index was used to select superior genotypes based on factor analysis and the genotype ideotype distance. Four factors explained the 11 characteristics studied, with a variability of 81.84%. Desirable gains were observed for 10 of the 11 traits evaluated. The FAI-BLUP index allowed the selection of superior seedless table grape hybrids, which can be used in the genetic improvement of grapevines. In conclusion, we report the selection of seedless table grape hybrids for our study to advance in the table grape breeding program in Brazilian semiarid regions. |
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Palavras-Chave: |
Uva de mesa; Uva sem semente. |
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Thesagro: |
Genótipo; Melhoramento Genético Vegetal; Uva. |
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Thesaurus NAL: |
Grapes; Table grapes. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/265970/1/Multitrait-selection-in-seedless-grape-hybrids-in-semiarid-regions-of-Brazil-2024.pdf
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Marc: |
LEADER 02036naa a2200265 a 4500
001 2154162
005 2024-04-26
008 2023 bl uuuu u00u1 u #d
024 7 $a10.1002/csc2.20990$2DOI
100 1 $aCARVALHO, J. N. de
245 $aMultitrait selection in seedless grape hybrids in semiarid regions of Brazil.$h[electronic resource]
260 $c2023
520 $aThe absence of seeds has been one of the most valued traits in grapes (Vitis spp.) for fresh consumption. However, the simultaneous selection of superior genotypes for multiple traits is complex. The objective of this study was to select genotypes of superior seedless table grapes in semiarid regions of Brazil. Multiple traits were considered by using the factor analysis and genotype?ideotype best linear unbiased prediction (FAI-BLUP) selection index. A total of 110 grape progenies from 24 crosses were evaluated for traits of agronomic interest. Each hybrid was represented by a single vine plant, without experimental design or repetition. The mixed-model methodology was used to predict the breeding values. The FAI-BLUP index was used to select superior genotypes based on factor analysis and the genotype ideotype distance. Four factors explained the 11 characteristics studied, with a variability of 81.84%. Desirable gains were observed for 10 of the 11 traits evaluated. The FAI-BLUP index allowed the selection of superior seedless table grape hybrids, which can be used in the genetic improvement of grapevines. In conclusion, we report the selection of seedless table grape hybrids for our study to advance in the table grape breeding program in Brazilian semiarid regions.
650 $aGrapes
650 $aTable grapes
650 $aGenótipo
650 $aMelhoramento Genético Vegetal
650 $aUva
653 $aUva de mesa
653 $aUva sem semente
700 1 $aCARVALHO, P. A. de
700 1 $aPIO, R.
700 1 $aBARBOSA, M. A. G.
700 1 $aLEAO, P. C. de S.
773 $tCrop Science$gv. 63, n. 4, p. 2091-2102, 2023.
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