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| 142. |  | SILVA, G. M. da; LAMEGO, F. P.; RITA, G. R.; PILON, M.; ROCHA, S. J. S. Beneficiamento de sementes de trevo vermelho (Trifolium pratense L.) visando à remoção de trevo branco (Trifolium repens L.). Informativo Abrates, v. 27, n. 2, p. 298, ago. 2017. Edição dos anais do 20º Congresso Brasileiro de Sementes, Foz do Iguaçu.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 144. | | MARTINS, I. M.; SILVA, R. D. D.; SILVA, E. F. da; SCURO, V. M.; OLIVEIRA, J. C. P. Avaliação de sementes de cevadilha vacariana. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 26.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 3., 2017, Pelotas. Anais... Pelotas: UFPel, 2017. CIC.| Biblioteca(s): Embrapa Pecuária Sul. |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Cerrados. |
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Data corrente: |
20/09/2022 |
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Data da última atualização: |
08/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
STEPHAN, M. P.; COSTA, A. M.; AZEVEDO, T. de L.; ROSA, J. S. da; SANTOS, A. A. dos. |
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Afiliação: |
MARILIA PENTEADO STEPHAN, CTAA; ANA MARIA COSTA, CPAC; TATIANA DE LIMA AZEVEDO, CTAA; JEANE SANTOS DA ROSA, CTAA; ALEXSANDRO ARAUJO DOS SANTOS, CTAA. |
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Título: |
Electrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Agricultural Science and Technology B, v. 12, n. 2, p. 40-47, 2022. |
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DOI: |
https://doi.org/10.17265/2161-6264/2022.02.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Brazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them. MenosBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as ... Mostrar Tudo |
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Palavras-Chave: |
Bioactive peptides; Mass distribution on fruit; P setacea; P tenuifila. |
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Thesagro: |
Casca; Eletroforese; Fruto; Maracujá; Método de Análise; Peptídeo; Polpa; Proteína; Purificação; Rastreamento; Semente; Tecnologia de Alimento. |
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Thesaurus NAL: |
Analytical methods; Bioactive compounds; Food technology; Fruits; Passiflora; Passion fruits; Peptides; Polyacrylamide gel electrophoresis; Proteins; Pulp; Purification methods; Seeds. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146660/1/62ea112170472.pdf
|
|
Marc: |
LEADER 03088naa a2200517 a 4500
001 2146660
005 2022-12-08
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.17265/2161-6264/2022.02.002$2DOI
100 1 $aSTEPHAN, M. P.
245 $aElectrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.$h[electronic resource]
260 $c2022
520 $aBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them.
650 $aAnalytical methods
650 $aBioactive compounds
650 $aFood technology
650 $aFruits
650 $aPassiflora
650 $aPassion fruits
650 $aPeptides
650 $aPolyacrylamide gel electrophoresis
650 $aProteins
650 $aPulp
650 $aPurification methods
650 $aSeeds
650 $aCasca
650 $aEletroforese
650 $aFruto
650 $aMaracujá
650 $aMétodo de Análise
650 $aPeptídeo
650 $aPolpa
650 $aProteína
650 $aPurificação
650 $aRastreamento
650 $aSemente
650 $aTecnologia de Alimento
653 $aBioactive peptides
653 $aMass distribution on fruit
653 $aP setacea
653 $aP tenuifila
700 1 $aCOSTA, A. M.
700 1 $aAZEVEDO, T. de L.
700 1 $aROSA, J. S. da
700 1 $aSANTOS, A. A. dos
773 $tJournal of Agricultural Science and Technology B$gv. 12, n. 2, p. 40-47, 2022.
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Embrapa Agroindústria de Alimentos (CTAA) |
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