Data/hora: 01/06/2024 09:22:31

Biblioteca(s): | Embrapa Milho e Sorgo.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 2
Data corrente: | 15/03/2022
Data da última atualização: | 22/03/2023
Autoria: | NASCIMENTO, P. T.; FADINI, M. A. M.; ROCHA, M S.; SOUZA, C. S. F.; BARROS, B. de A.; MELO, J. O. F.; VON PINHO, R. G.; VALICENTE, F. H.
Afiliação: | Universidade Federal de Lavras; Universidade Federal de São João del-Rei; Universidade Federal de São João del-Rei; Universidade Federal de Lavras; BEATRIZ DE ALMEIDA BARROS, CNPMS; Universidade Federal de São João del-Rei; Universidade Federal de Lavras; FERNANDO HERCOS VALICENTE, CNPMS.
Título: | Olfactory response of Trichogramma pretiosum (Hymenoptera: Trichogrammatidae) to volatiles induced by transgenic maize.
Ano de publicação: | 2021
Fonte/Imprenta: | Bulletin of Entomological Research, v. 111, p. 674-687, 2021.
DOI: | https://doi.org/10.1017/S0007485321000341
Idioma: | Inglês
Conteúdo: | Plants not only respond to herbivorous damage but adjust their defense system afteregg deposition by pest insects. Thereby, parasitoids use oviposition-induced plant volatiles to locate their hosts. We investigated the olfactory behavioral responses of Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae) to volatile blends emitted by maize (Zea mays L.) with singular and stacked events after oviposition by Spodoptera frugiperda Smith, 1797 (Hymenoptera: Trichogrammatidae) moths. Additionally, we examined possible variations in gene expression and on oviposition-induced volatiles. We used a Y-tube olfactometer to test for the wasp responses to volatiles released by maize plants oviposited by S. frugiperda and not-oviposited plants. Using the real-time PCR technique (qRT-PCR), we analyzed the expression of lipoxygenase and three terpene synthases genes, which are enzymes involved in the synthesis of volatile compounds that attract parasitoids of S. frugiperda. Olfactometer tests showed that T. pretiosum is strongly attracted by volatiles from transgenic maize emitted by S. frugiperda oviposition (VTPRO 3, more than 75% individuals were attracted). The relative expression of genes TPS10, LOX e STC was higher in transgenic hybrids than in the conventional (isogenic line) hybrids. The GC-MS analysis revealed that some volatile compounds are released exclusively by transgenic maize. This study provides evidence that transgenic hybrids enhanced chemical cues under oviposition-induction and helped to increase T. pretiosum efficiency in S. frugiperda control. This finding shows that among the evaluated hybrids, genetically modified hybrids can improve the biological control programs, since they potentialize the egg parasitoid foraging, integrating pest management.
Palavras-Chave: | Egg parasitoid; Empilhamento de características; Genetic manipulation; Interação tritrófica; Manipulação genética; Parasitóide de ovo; QRT-PCR; Trait stacking; Tritrophic interaction.
Thesagro: | Inseto Para Controle Biológico; Milho; Planta Transgênica; Trichogramma sp.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (29785-1) - UPC - AP - DD -  -  -  - DIS