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| 163. |  | OLIVEIRA, D. R. de; SANTOS, V. B. dos; BEZERRA, S. A.; SOUZA, A. K.; SANTOS, R. C.; SIVIERO, A.; MARINHO, J. T. de S.; NASCIMENTO, F. S. S.; SILVA, R. da. Variabilidade de variedades tradicionais de feijão comum do Acre. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 11., 2014. Londrina. Anais... Londrina: IAPAR, 2014. 3 p.| Biblioteca(s): Embrapa Acre. |
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| 169. | | CARVALHO, M. da C. S.; FERREIRA, G. B.; MUTADIUA, C. A. P.; DENARDIN, J. E.; MIRANDA, C. H. B.; FAVARO, S. P.; SILVA, H. R. da; MELO, L. C.; SILVA FILHO, P. M. da; NEUMAIER, N.; CRUZ, I.; ROCHA, M. de M.; ALMEIDA, R. P. de; MUSSA, V.; DAMBA, G.; KAUNDA, J. B. Adubação do feijão-vulgar com fósforo e potássio em Lichinga, Niassa, Moçambique: resultados de duas campanhas agrícolas e recomendações preliminares. In: SEMINÁRIO DE DIVULGAÇÃO DE RESULTADOS DA INVESTIGAÇÃO AGRÁRIA NO CORREDOR DE NACALA, 2., 2015, Lichinga, Moçambique. Anais... Lichinga, Moçambique: Instituto de Investigação Agrária de Moçambique, 2015. Editado por Fernando João Sualei, Oscar Chichongue, Guilhermino Boina, Simone Palma Favaro, Cesar Heraclides Behling Miranda. p. 280-290.| Biblioteca(s): Embrapa Trigo. |
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| 172. | | CORREA, C. M. de A.; CORREA, A. M.; ABOT, A. R.; NASCIMENTO, J. N. do; RODRIGUES, S. R.; OLIVEIRA, H. N. de. Avaliação dos danos do complexo de lagartas-das- vagens em genótipos de feijão-caupi (Vigna unguiculata), em Aquidauana, MS In: SEMANA AGRONÔMICA, 6.; ENCONTRO TÉCNICO-CIENTÍFICO, 1., 2009, Aquidauana. [Resumos...]. Aquidauana: UEMS, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Agropecuária Oeste. |
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| 175. | | NASCENTE, A. S.; DANTAS, D. DOS S.; COBUCCI, T.; LIMA, D. A. DE P.; KLUTHCOUSKI, J.; OLIVEIRA, P. de. Avaliação de Biozyme na produtividade do feijoeiro. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão, 2011.| Biblioteca(s): Embrapa Cerrados. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
|
Data corrente: |
10/01/2005 |
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Data da última atualização: |
03/08/2017 |
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Autoria: |
GRANGE, L.; HUNGRIA, M. |
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Título: |
Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. MenosAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo |
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Palavras-Chave: |
Rizóbio. |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
LEADER 02618naa a2200157 a 4500
001 1467760
005 2017-08-03
008 2004 bl --- 0-- u #d
100 1 $aGRANGE, L.
245 $aGenetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems.
260 $c2004
520 $aAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena.
650 $aFeijão
653 $aRizóbio
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 36, n. 9, p. 1389-1398, Sept. 2004.
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