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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
01/12/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOTA, F. F. da; GOMES, E. A.; SELDIN, L. |
Afiliação: |
Fabio Faria da Mota, UFRJ; ELIANE APARECIDA GOMES, CNPMS; Lucy Seldin, UFRJ. |
Título: |
Auxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Journal of Microbiology, v. 46, n. 3, p. 257-264, 2008. |
DOI: |
10.1007/s12275-007-0245-x |
Idioma: |
Inglês |
Conteúdo: |
Different species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome. MenosDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not pre... Mostrar Tudo |
Palavras-Chave: |
Auxin; Auxin efflux earrier protein; Indole-3-aeetic acid; Paenibacillus graminis. |
Thesaurus Nal: |
Paenibacillus polymyxa. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02470naa a2200217 a 4500 001 1491541 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1007/s12275-007-0245-x$2DOI 100 1 $aMOTA, F. F. da 245 $aAuxin production and detection of the gene coding for the auxin effux carrier (AEC) protein in Paenibacillus polymyxa.$h[electronic resource] 260 $c2008 520 $aDifferent species of Paenibacillus are considered to be plant growth-promoting rhizobacteria (pGPR) due to their ability to repress soil borne pathogens, fix atmospheric nitrogen, induce plant resistance to diseases and/for produce plant growth-regulating substances such as auxins. Although it is known that indole-3-acetic acid (lAA) is the primary naturally occurring auxin excreted by Paenibacülus species, its transport mechanisms (auxin effiux carriers) have not yet been characterized. In this study, the auxin production of P. polymyxa and P. graminis, which are prevalent in the rhizospheres of maize and sorghum sown in Brazil, was evaluated. In addition, the gene encoding the Auxin Effiux Carrier (AEC) protein from P. polymyxa DSM36T was sequenced and used to determine if various strains of P. polymyxa and P. graminis possessed this gene. Each of the 68 P. polymyxa strains evaluated in this study was able to produce IAA, which was produced at concentrations varyjng from 1 to 17 ug/ml. However, auxin production was not detected in any of the 13 P. graminis strains tested in this study. Dift'erent primers were designed for the PCR amplification of the gene coding for the AEC in P. polymyxa, and the predicted protein of 319 aa was homologous to AEC from Bacillus amyloliquefaciens, B. licheniformis, and B. subtilis. However, no product was observed when these primers were used to amplify the genomic DNA of seven strains of P. graminis, which suggests that this gene is not present in this species. Moreover, none of the P. graminis genomes tested were homol-ogous to the gene coding for AEC, whereas ali of the P. polymyxa genomes evaluated were. This is the first study to demonstrate that the AEC protein is present in P. polymyxa genome. 650 $aPaenibacillus polymyxa 653 $aAuxin 653 $aAuxin efflux earrier protein 653 $aIndole-3-aeetic acid 653 $aPaenibacillus graminis 700 1 $aGOMES, E. A. 700 1 $aSELDIN, L. 773 $tJournal of Microbiology$gv. 46, n. 3, p. 257-264, 2008.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
22/02/1999 |
Data da última atualização: |
16/05/2011 |
Autoria: |
LOPES, O. M. N. |
Afiliação: |
OTAVIO MANOEL NUNES LOPES, CPATU. |
Título: |
Feijão-de-porco leguminosa para adubação verde e cobertura de solo. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1998. |
Páginas: |
4 p. |
Série: |
(EMBRAPA-CPATU. Recomendações básicas, 37). |
Idioma: |
Português |
Conteúdo: |
Introdução; Características da planta; Semeadura; Tratos culturais; Produção de biomassa; Concentração de nutrientes; Quantidade de nutrientes na materia seca; Produção de sementes; Considerações finais. |
Palavras-Chave: |
Agronomic chacteristics; Belem; Brasil; Caracteristica agronomica; Cobertura de solo; Cover plants; Cultivo; Green manuring; Para; Producao de biomassa; Producao de semente; Soil reclamation plants. |
Thesagro: |
Adubação Verde; Canavalia Ensiformis; Cobertura do Solo; Cobertura Vegetal; Conservação do Solo; Feijão de Porco; Leguminosa; Manejo; Nutriente; Solo. |
Thesaurus NAL: |
Amazonia; biomass; crop management; green manures; legumes; nutrients; soil; soil conservation. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/34710/1/RecBas-37.pdf
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Marc: |
LEADER 01505nam a2200493 a 4500 001 1375234 005 2011-05-16 008 1998 bl uuuu u0uu1 u #d 100 1 $aLOPES, O. M. N. 245 $aFeijão-de-porco leguminosa para adubação verde e cobertura de solo. 260 $aBelém, PA: EMBRAPA-CPATU$c1998 300 $a4 p. 490 $a(EMBRAPA-CPATU. Recomendações básicas, 37). 520 $aIntrodução; Características da planta; Semeadura; Tratos culturais; Produção de biomassa; Concentração de nutrientes; Quantidade de nutrientes na materia seca; Produção de sementes; Considerações finais. 650 $aAmazonia 650 $abiomass 650 $acrop management 650 $agreen manures 650 $alegumes 650 $anutrients 650 $asoil 650 $asoil conservation 650 $aAdubação Verde 650 $aCanavalia Ensiformis 650 $aCobertura do Solo 650 $aCobertura Vegetal 650 $aConservação do Solo 650 $aFeijão de Porco 650 $aLeguminosa 650 $aManejo 650 $aNutriente 650 $aSolo 653 $aAgronomic chacteristics 653 $aBelem 653 $aBrasil 653 $aCaracteristica agronomica 653 $aCobertura de solo 653 $aCover plants 653 $aCultivo 653 $aGreen manuring 653 $aPara 653 $aProducao de biomassa 653 $aProducao de semente 653 $aSoil reclamation plants
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