Data/hora: 03/06/2024 15:47:06

Biblioteca(s): | Embrapa Arroz e Feijão.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 18/03/2024
Data da última atualização: | 18/03/2024
Autoria: | SILVA, R. S.; FARIA, J. C.; VIANELLO, R. P.; VALDISSER, P. A. M. R.; PEREIRA, H. S.; MELO, L. C.; PINHEIRO, P. V.; SOUZA, T. L. P. O.
Afiliação: | RODRIGO S. SILVA, UFG; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; HELTON SANTOS PEREIRA, CNPAF; LEONARDO CUNHA MELO, CNPAF; PATRICIA VALLE PINHEIRO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF.
Título: | Inheritance and genetic mapping of the first CPMMV tolerance locus in common bean.
Ano de publicação: | 2024
Fonte/Imprenta: | Plant Pathology, 2024.
DOI: | https://doi.org/10.1111/ppa.13892
Idioma: | Inglês
Notas: | Early view.
Conteúdo: | Although cowpea mild mottle virus (CPMMV) has been reported in Brazil since 1983, it has only become a significant concern for researchers and farmers in recent years. The objective of this work was to investigate the genetic basis of CPMMV tolerance, mapping and reporting the first loci associated with this trait in common bean (cv. BRS Sublime). Phenotypic assays were carried out on 180 individual plants (F2 generation) and 180 F2:3 progenies comprising 12 plants per family and their parents (BRS Sublime, tolerant parent × CNFCT 16207, susceptible parent). CPMMV was mechanically inoculated and symptoms were evaluated at 35 days after inoculation, using a 1–5 scoring scale. A linkage map was constructed using 1695 single-nucleotide polymorphism (SNP) and SilicoDArT markers that segregated in the F2 and F2:3 generations as expected. Markers were distributed across 11 common bean chromosomes, resulting in a total length of 2864 cM, with an average distance between markers of 1.8 cM. Phenotypic observations revealed that tolerance in cv. BRS Sublime is controlled by a single dominant gene. The main effect quantitative trait locus (QTL; CPMMV.Pv08) associated with CPMMV tolerance was identified in the terminal region on chromosome 8 (Pv08). This QTL explained approximately 77% of phenotypic variation, indicating that the inheritance of tolerance to CPMMV is monogenic, controlled by a major locus. As far as we know, this study represents the first investigation into the inheritance and genetic mapping of CPMMV tolerance in common bean, with potential for the development of elite lines with multiple virus resistance/tolerance.
Palavras-Chave: | DArTSeq; SNP markers.
Thesagro: | Bemisia Tabaci; Feijão; Marcador Genético; Phaseolus Vulgaris; Vírus.
Thesaurus Nal: | Carlavirus; Cowpea mild mottle virus; Inheritance (genetics); Monogenic inheritance.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (36943-1) - UPC - AP - DD - 2024 -  - 2024 - DIS