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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
13/12/2024 |
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Data da última atualização: |
05/02/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, R. M. de; FERNANDES, C. A. C.; SIQUEIRA, L. G. B.; FIGUEIREDO, R. A.; MARTINS, C. F.; PEIXER, M. A. S.; XAVIER, M. C.; VIANA, J. H. M. |
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Afiliação: |
RODRIGO MARTINS DE MOURA, UNIVERSIDADE DE BRASÍLIA; CARLOS ANTONIO CARVALHO FERNANDES, UNIVERSIDADE EDSON ANTÔNIO VELANO; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; RICARDO ALAMINO FIGUEIREDO, CENARGEN; CARLOS FREDERICO MARTINS, CPAC; MAURÍCIO ANTONIO SILVA PEIXER, BIO BIOTECNOLOGIA EM REPRODUÇÃO ANIMAL LTDA; MARCELO CUNHA XAVIER, BIO BIOTECNOLOGIA EM REPRODUÇÃO ANIMAL LTDA; JOAO HENRIQUE MOREIRA VIANA, CENARGEN. |
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Título: |
Ovarian stimulation of Nelore calves and prepubertal heifers with a long-acting recombinant human FSH (corifollitropin-alpha) and subsequent ovum pick-up and in vitro embryo production outcomes. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Theriogenology, v. 234, p. 110-116, 2025. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2024.12.011 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the use of a long-acting recombinant human FSH (rhFSH, corifollitropin-alpha) to induce ovarian stimulation in Nelore breed (Bos indicus) calves and prepubertal heifers prior to ovum pick-up (OPU) for in vitro embryo production (IVEP). In Experiment 1, a dose-response trial was performed to determine the optimal dose of rhFSH, which was determined to be 10 μg. In Experiment 2, 6-7 mo old calves were randomly allocated to receive rhFSH either via sc (n=5) or im (n=5). Ovarian follicular development was monitored daily by transrectal ultrasonography for five days. There was no effect of route (P=0.1348) nor route x time interaction (P=0.8336) on follicle development. In Experiment 3, 7-8 mo old calves (n=90) were randomly allocated into 5 groups: 1) Control: no ovarian stimulation; 2) rhFSH-96: 10 μg rhFSH sc followed by OPU 96h later; 3) rhFSH-120: 10 μg rhFSH sc and OPU 120h later; 4) eCG-96: 300 IU eCG im and OPU 96h later; and 5) eCG-120: 300 IU eCG im and OPU 120h later. Non- rhFSH treated Nelore mature cows (n=10) were used as reference-controls for IVEP outcomes. Treatment with rhFSH increased the proportion of grade I cumulus-oocyte complexes (COC) collected by OPU at either timepoints (96 or 120h) compared with eCG or controls (P<0.0001). Blastocyst rate for rhFSH-120 calves was similar to mature cows (P>0.05). Treatment with rhFSH, however, increased the proportion of expanded COC and decreased the proportion of viable COC (P<0.0001) compared with eCG and controls. In Experiment 4, yearling heifers (n=60) were treated or not (control group) with 10 μg rhFSH sc and OPU was performed 72 or 96h later. Heifers treated with rhFSH had a greater proportion of grade I COC (P=0.0188) and blastocyst rate (P<0.0098) than controls, regardless of the interval used. These groups, however, yielded a lesser number of viable COC (P=0.0264), resulting in a similar (P=0.5869) number of embryos produced by donor per OPU compared with controls. Pregnancy rate after embryo transfer was also similar between controls and rhFSH groups (19.3 vs 25.0%, P=0.4142). In summary, treatment with a single sc injection of corifollitropin-alpha was effective to promote ovarian stimulation in calves prior to OPU. The potential benefits of stimulatory protocols using rhFSH, however, have been overshadowed by a decrease in the total number of viable COC recovered per donor, thus failing to increase the number of embryos produced per OPU. MenosThe aim of this study was to evaluate the use of a long-acting recombinant human FSH (rhFSH, corifollitropin-alpha) to induce ovarian stimulation in Nelore breed (Bos indicus) calves and prepubertal heifers prior to ovum pick-up (OPU) for in vitro embryo production (IVEP). In Experiment 1, a dose-response trial was performed to determine the optimal dose of rhFSH, which was determined to be 10 μg. In Experiment 2, 6-7 mo old calves were randomly allocated to receive rhFSH either via sc (n=5) or im (n=5). Ovarian follicular development was monitored daily by transrectal ultrasonography for five days. There was no effect of route (P=0.1348) nor route x time interaction (P=0.8336) on follicle development. In Experiment 3, 7-8 mo old calves (n=90) were randomly allocated into 5 groups: 1) Control: no ovarian stimulation; 2) rhFSH-96: 10 μg rhFSH sc followed by OPU 96h later; 3) rhFSH-120: 10 μg rhFSH sc and OPU 120h later; 4) eCG-96: 300 IU eCG im and OPU 96h later; and 5) eCG-120: 300 IU eCG im and OPU 120h later. Non- rhFSH treated Nelore mature cows (n=10) were used as reference-controls for IVEP outcomes. Treatment with rhFSH increased the proportion of grade I cumulus-oocyte complexes (COC) collected by OPU at either timepoints (96 or 120h) compared with eCG or controls (P<0.0001). Blastocyst rate for rhFSH-120 calves was similar to mature cows (P>0.05). Treatment with rhFSH, however, increased the proportion of expanded COC and decreased the proportion of viable COC (P<0.0... Mostrar Tudo |
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Palavras-Chave: |
In vitro embryo production; Recombinant gonadotropins. |
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Thesagro: |
Bos Indicus; Embrião Animal; Gonadotrofina; Reprodução Animal. |
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Thesaurus Nal: |
Puberty. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03524naa a2200301 a 4500
001 2171620
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008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2024.12.011$2DOI
100 1 $aMOURA, R. M. de
245 $aOvarian stimulation of Nelore calves and prepubertal heifers with a long-acting recombinant human FSH (corifollitropin-alpha) and subsequent ovum pick-up and in vitro embryo production outcomes.$h[electronic resource]
260 $c2025
520 $aThe aim of this study was to evaluate the use of a long-acting recombinant human FSH (rhFSH, corifollitropin-alpha) to induce ovarian stimulation in Nelore breed (Bos indicus) calves and prepubertal heifers prior to ovum pick-up (OPU) for in vitro embryo production (IVEP). In Experiment 1, a dose-response trial was performed to determine the optimal dose of rhFSH, which was determined to be 10 μg. In Experiment 2, 6-7 mo old calves were randomly allocated to receive rhFSH either via sc (n=5) or im (n=5). Ovarian follicular development was monitored daily by transrectal ultrasonography for five days. There was no effect of route (P=0.1348) nor route x time interaction (P=0.8336) on follicle development. In Experiment 3, 7-8 mo old calves (n=90) were randomly allocated into 5 groups: 1) Control: no ovarian stimulation; 2) rhFSH-96: 10 μg rhFSH sc followed by OPU 96h later; 3) rhFSH-120: 10 μg rhFSH sc and OPU 120h later; 4) eCG-96: 300 IU eCG im and OPU 96h later; and 5) eCG-120: 300 IU eCG im and OPU 120h later. Non- rhFSH treated Nelore mature cows (n=10) were used as reference-controls for IVEP outcomes. Treatment with rhFSH increased the proportion of grade I cumulus-oocyte complexes (COC) collected by OPU at either timepoints (96 or 120h) compared with eCG or controls (P<0.0001). Blastocyst rate for rhFSH-120 calves was similar to mature cows (P>0.05). Treatment with rhFSH, however, increased the proportion of expanded COC and decreased the proportion of viable COC (P<0.0001) compared with eCG and controls. In Experiment 4, yearling heifers (n=60) were treated or not (control group) with 10 μg rhFSH sc and OPU was performed 72 or 96h later. Heifers treated with rhFSH had a greater proportion of grade I COC (P=0.0188) and blastocyst rate (P<0.0098) than controls, regardless of the interval used. These groups, however, yielded a lesser number of viable COC (P=0.0264), resulting in a similar (P=0.5869) number of embryos produced by donor per OPU compared with controls. Pregnancy rate after embryo transfer was also similar between controls and rhFSH groups (19.3 vs 25.0%, P=0.4142). In summary, treatment with a single sc injection of corifollitropin-alpha was effective to promote ovarian stimulation in calves prior to OPU. The potential benefits of stimulatory protocols using rhFSH, however, have been overshadowed by a decrease in the total number of viable COC recovered per donor, thus failing to increase the number of embryos produced per OPU.
650 $aPuberty
650 $aBos Indicus
650 $aEmbrião Animal
650 $aGonadotrofina
650 $aReprodução Animal
653 $aIn vitro embryo production
653 $aRecombinant gonadotropins
700 1 $aFERNANDES, C. A. C.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aFIGUEIREDO, R. A.
700 1 $aMARTINS, C. F.
700 1 $aPEIXER, M. A. S.
700 1 $aXAVIER, M. C.
700 1 $aVIANA, J. H. M.
773 $tTheriogenology$gv. 234, p. 110-116, 2025.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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