| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
27/10/2015 |
|
Data da última atualização: |
18/05/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S. |
|
Afiliação: |
Á. M. VIDAL, UFRB; L. J. VIEIRA; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
|
Título: |
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015. |
|
ISSN: |
1676-5680 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. MenosMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava p... Mostrar Tudo |
|
Palavras-Chave: |
Marcado molecular. |
|
Thesagro: |
Anatomia vegetal; Banco de germoplasma; Mandioca; Meristema. |
|
Thesaurus Nal: |
Cassava; Genetic markers; Germplasm. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | SMITH, M. N.; SCHITTI, J.; GONÇALVES, N.; MINOR, D.; ALMEIDA, D. R. A. de; ROCHA, D. G.; ARAGÓN, S.; MENIN, M.; GUEDES, M. C.; TONINI, H.; SILVA, K. E. da; ROSA, D. M.; NELSON, B. W.; CORDEIRO, C. L. O.; OLIVEIRA JUNIOR, R. C. de; SHAO, G.; SOUZA, M. S.; MCMAHON, S.; ALMEIDA, D.; ARAGÃO, L. E. O. C.; LIMA, N. Z. de; OLIVEIRA, G. de; ASSIS, R. L. de; CAMARGO, J. L.; MESQUITA, R. G.; SALESKA, S. R.; BRESHEARS, D. D.; COSTA, F. R. C.; STARK, S. C. Variations in Amazonian forest canopy structure and light environments across environmental and disturbance gradients. In: AGU FALL MEETING, 2019, San Francisco. Anais... San Francisco: AGU, 2019. Paper 499657.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Pecuária Sul. |
|    |
| 2. | | SMITH, M. N.; STARK, S. C.; TAYLOR, T. C.; SCHIETTI, J.; ALMEIDA, D. R. A. de; ARAGÓN, S.; TORRALVO, K.; LIMA, A. P.; OLIVEIRA, G. de; ASSIS, R. L. de; LEITOLD, V.; PONTES-LOPES, A.; SCOLES, R.; VIEIRA, L. C. de S.; RESENDE, A. F.; COPPOLA, A. I.; BRANDÃO, D. O.; SILVA JUNIOR, J. de A.; LOBATO, L. F.; FREITAS, W.; ALMEIDA, D.; SOUZA, M. S.; MINOR, D. M.; VILLEGAS, J. C.; LAW, D. J.; GONÇALVES, N.; ROCHA, D. G. da; GUEDES, M. C.; TONINI, H.; SILVA, K. E. da; HAREN, J. van; ROSA, D. M.; VALLE, D. F. do; CORDEIRO, C. L.; LIMA, N. Z. de; SHAO, G.; MENOR, I. O.; CONTI, G.; FLORENTINO, A. P.; MONTTI, L.; ARAGÃO, L. E. O. C.; McMAHON, S. M.; PARKER, G. G.; BRESHEARS, D. D.; COSTA, A. C. L. da; MAGNUSSON, W. E.; MESQUITA, R.; CAMARGO, J. L. C.; OLIVEIRA JUNIOR, R. C. de; CAMARGO, P. B. de; SALESKA, S. R.; NELSON, B. W. Diverse anthropogenic disturbances shift Amazon forests along a structural spectrum. Frontiers in Ecology an the Environment, v. 21, n. 1, p. 24-32, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Pecuária Sul. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
