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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
27/10/2010 |
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Data da última atualização: |
04/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, G. T. C. P.; CARNEIRO, N. P.; KARTHIKEYAN, A. S.; RAGHOTHAMA, K. G.; SCHAFFERT, R. E.; BRANDAO, R. L.; PAIVA, L. V.; SOUZA, I. R. P. de; ALVES, V. M.; IMOLESI, A.; CARVALHO, C. H. S.; CARNEIRO, A. A. |
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Afiliação: |
Gracielle T. C. P. Coelho, UFLA; NEWTON PORTILHO CARNEIRO, CNPMS; ATHIKKATTUVALASU S. KARTHIKEYAN, Purdue University; KASCHANDRA G. RAGHOTHAMA, Purdue University; ROBERT EUGENE SCHAFFERT, CNPMS; ROSANGELA L. BRANDÃO, UFLA; LUCIANO V. PAIVA, UFLA; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; ANDERSON IMOLESI; CARLOS H. S. CARVALHO; ANDREA ALMEIDA CARNEIRO, CNPMS. |
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Título: |
A phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, Athens, v. 28, p. 717-723, 2010. |
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DOI: |
10.1007/s1 I 105-010-0199-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Phosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions. |
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Thesagro: |
Fósforo; Genética vegetal. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01970naa a2200289 a 4500
001 1865352
005 2018-06-04
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1007/s1 I 105-010-0199-8$2DOI
100 1 $aCOELHO, G. T. C. P.
245 $aA phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation.$h[electronic resource]
260 $c2010
520 $aPhosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.
650 $aFósforo
650 $aGenética vegetal
700 1 $aCARNEIRO, N. P.
700 1 $aKARTHIKEYAN, A. S.
700 1 $aRAGHOTHAMA, K. G.
700 1 $aSCHAFFERT, R. E.
700 1 $aBRANDAO, R. L.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA, I. R. P. de
700 1 $aALVES, V. M.
700 1 $aIMOLESI, A.
700 1 $aCARVALHO, C. H. S.
700 1 $aCARNEIRO, A. A.
773 $tPlant Molecular Biology Reporter, Athens$gv. 28, p. 717-723, 2010.
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