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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALLES, H. O.; VASCONCELOS, I. M.; SANTOS, L. F. L.; OLIVEIRA, H. D.; COSTA, P. P. C.; NASCIMENTO, N. R. F.; SANTOS, C. F.; SOUSA, D. F.; JORGE, A. R. C.; MENEZES, D. B.; MONTEIRO, H. S. A.; GONDIM, D. M. F.; OLIVEIRA, J. T. A. |
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Afiliação: |
HEVILA OLIVEIRA SALLES FIGUEIREDO, CNPC; Universidade Federal do Ceará (UFC), Fortaleza, CE.; Universidade Estadual do Ceará (UECe) - Fortaleza, CE.; UECe; UECe; UECe; UECe; UFC, Fortaleza, CE.; UFC; UFC; UFC; UFC; UFC. |
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Título: |
Towards a better understanding of Ipomoea asarifolia toxicity: evidence of the involvement of a leaf lectin. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Toxicon, v. 58, p. 502-508, 2011. |
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DOI: |
10.1016/j.toxicon.2011.08.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves. MenosAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination o... Mostrar Tudo |
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Palavras-Chave: |
Ipomoea asarifolia; Lectin; Sulato de amônia. |
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Thesagro: |
Erva daninha; Lectina; Planta tóxica; Salsa; Toxidez. |
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Thesaurus Nal: |
toxicity. |
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Categoria do assunto: |
-- |
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Marc: |
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100 1 $aSALLES, H. O.
245 $aTowards a better understanding of Ipomoea asarifolia toxicity$bevidence of the involvement of a leaf lectin.$h[electronic resource]
260 $c2011
520 $aAbstract: Natural intoxication of livestock by ingestion of Ipomoea asarifolia leaves has been reported to occur widely in Brazil. Previous studies carried out by our research group provided strong evidence that a lectin could be involved with the toxic properties of I. asarifolia.To reinforce this hypothesis, a lectin-enriched fraction (LEF) was isolated from I. asarifolia leaves and its toxic effects were assessed. Leaves of I. asarifolia were excised from plants growing widely in the ?eld, mechanically wounded and maintained in a chamber at 25 3 C for 72 h in the dark, under near 100% relative humidity. The leaf proteins were extracted, ammonium sulfate precipitated, chromatographed on DEAE-cellulose and Phenyl-Sepharose to produce LEF that under SDS?PAGE showed a molecular mass of 44.0 kDa and after N-terminal amino acid analysis a primary sequence composed of AGYTPVLDIGAEVLAAGEPY. The in vivo toxicity of LEF assessed by intraorbital injection in mice showed induced severe uncoordinated movements without death. LEF reduced the muscular contraction in a dose depend way and at 29.8 mg/mL (CE50) it produces 50% inhibition of contraction, suggesting that LEF blunts autonomic neurotransmission. Isolated rat kidneys were perfused with LEF and no effects on the perfusion pressure or renal vascular resistance were observed, but urinary ?ow and glomerular ?ltration rate increased. Moreover, the percentage of tubular transport of Naþ,Kþ and Cl decreased. Histological examination of the kidneys perfused with LEF exhibited little alterations. These toxic effects observed above were concomitant with the increase of LEF hemag-glutination activity, which strongly suggest that one of the toxic principles of I. asarifolia is a lectin present in its leaves.
650 $atoxicity
650 $aErva daninha
650 $aLectina
650 $aPlanta tóxica
650 $aSalsa
650 $aToxidez
653 $aIpomoea asarifolia
653 $aLectin
653 $aSulato de amônia
700 1 $aVASCONCELOS, I. M.
700 1 $aSANTOS, L. F. L.
700 1 $aOLIVEIRA, H. D.
700 1 $aCOSTA, P. P. C.
700 1 $aNASCIMENTO, N. R. F.
700 1 $aSANTOS, C. F.
700 1 $aSOUSA, D. F.
700 1 $aJORGE, A. R. C.
700 1 $aMENEZES, D. B.
700 1 $aMONTEIRO, H. S. A.
700 1 $aGONDIM, D. M. F.
700 1 $aOLIVEIRA, J. T. A.
773 $tToxicon$gv. 58, p. 502-508, 2011.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 5 | |
| 1. |  | MAUES, M. M.; LEÃO, N. V. M.; SOUSA, D. B. de. Fenologia reprodutiva de especies arbustivas e arbóreas de vegetação secundária em duas áreas de diferentes idades, no município de Igarapé-Açu (PA). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA CNPq/FCAP/EMBRAPA, 6., 1996, Belém, PA. Resumos. Belém, PA: FCAP, 1996. p. 85, 151. Publicado também em Congresso Brasileiro de Iniciação Científica em Ciências Agrárias, 16., 1996, Santa Maria, RS.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | SOUSA, D. B. de; MAUES, M. M.; LEÃO, N. V. M. Fenologia reprodutiva de espécies arbustivas e arbóreas em vegetação secundária no Nordeste paraense. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 5., 1995, Belém, PA. Resumos... Belém, PA: FCAP, Unidade de Apoio à Pesquisa, 1995. p. 58, 148.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | HERMES, F.; GIACOMIN, L.; OLIVEIRA JUNIOR, R. C. de; OLIVEIRA, D. R. de; OLIVEIRA, E. L. de; SOUSA, D. B. de. Comparação de densidades de plantio em cultivo de soja no oeste do Pará. In: CONGRESSO BRASILEIRO DE AGRONOMIA, 29., 2015, Foz do Iguaçu. Desafios e oportunidades profissionais: anais. Curitiba: CONFEA-PR, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | OLIVEIRA JUNIOR, R. C. de; SOUSA NETO, T. L. de; TAKETOMI, Y. C.; OLIVEIRA, D. R. de; SOUSA, D. B. de; TANABE, C. S. Quantificação de restos culturais e concentração de nutrientes na cultura do milho no Município de Belterra/PA. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | CONCEICAO, H. E. O. da; SILVA, E. S. A.; ROCHA NETO, O. G. da; STEIN, R. L. B.; SANTIAGO, E. J. A. de; SOUSA, D. B. de; GEMAQUE, R. C. R.; SOUZA, M. M. M. de. Método para estimar a área foliar do cupuaçuzeiro. In: SEMINÁRIO INTERNACIONAL SOBRE PIMENTA-DO-REINO E CUPUAÇU, 1996, Belém, PA. Anais... Belém, PA: EMBRAPA-CPATU: JICA, 1997. p. 325-331. (EMBRAPA-CPATU. Documentos, 89).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| Registros recuperados : 5 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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