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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/09/1996 |
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Data da última atualização: |
13/06/2023 |
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Autoria: |
TULI, R. K.; HOLTZ, W. |
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Título: |
Effect of glycerolization procedure and removal of seminal plasma on post-thaw survival and got-release from Boer goat spermatozoa. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Theriogenology, v.42, n.3, p.547-555, 1994. |
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DOI: |
10.1016/0093-691x(94)90692-c |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Forty ejaculates (20 for each of 2 experiments) were collected from 4 Boer goat bucks at weekly intervals to study the effect of glycerolization procedure and removal of seminal plasma on progressive motility, percent live spermatozoa and release of glutamic oxaloacetic transaminase (GOT) before and after the freezing of semen. Stepwise glycerolization at 37 degrees C gave higher progressive motility and percentage of live spermatozoa both before freezing and after thawing than one-step glycerolization at 37 degrees C or stepwise extension with glycerol being added after cooling to 5 degrees C. The GOT-release was reduced before freezing and after thawing of semen with stepwise glycerolization (P<0.05). Progressive motility and the percentage of live spermatozoa were higher (P<0.05) after the freezing of whole semen than in washed spermatozoa. The concentration of GOT in the extra-cellular fluid was lower in washed spermatozoa prior to freezing (P<0,05); but after thawing, the washed spermatozoa released more GOT than spermatozoa in whole semen. Removal of seminal plasma prior to freezing spermatozoa in an extender containing egg yolk had an unfavorable effect on their post-thaw motility and integrity. |
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Palavras-Chave: |
Glicerol; GOT. |
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Thesagro: |
Caprino; Criopreservação; Sêmen. |
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Thesaurus Nal: |
Cryopreservation; Glycerol; Goats; Reproduction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01967naa a2200253 a 4500
001 1522496
005 2023-06-13
008 1994 bl uuuu u00u1 u #d
024 7 $a10.1016/0093-691x(94)90692-c$2DOI
100 1 $aTULI, R. K.
245 $aEffect of glycerolization procedure and removal of seminal plasma on post-thaw survival and got-release from Boer goat spermatozoa.$h[electronic resource]
260 $c1994
520 $aAbstract: Forty ejaculates (20 for each of 2 experiments) were collected from 4 Boer goat bucks at weekly intervals to study the effect of glycerolization procedure and removal of seminal plasma on progressive motility, percent live spermatozoa and release of glutamic oxaloacetic transaminase (GOT) before and after the freezing of semen. Stepwise glycerolization at 37 degrees C gave higher progressive motility and percentage of live spermatozoa both before freezing and after thawing than one-step glycerolization at 37 degrees C or stepwise extension with glycerol being added after cooling to 5 degrees C. The GOT-release was reduced before freezing and after thawing of semen with stepwise glycerolization (P<0.05). Progressive motility and the percentage of live spermatozoa were higher (P<0.05) after the freezing of whole semen than in washed spermatozoa. The concentration of GOT in the extra-cellular fluid was lower in washed spermatozoa prior to freezing (P<0,05); but after thawing, the washed spermatozoa released more GOT than spermatozoa in whole semen. Removal of seminal plasma prior to freezing spermatozoa in an extender containing egg yolk had an unfavorable effect on their post-thaw motility and integrity.
650 $aCryopreservation
650 $aGlycerol
650 $aGoats
650 $aReproduction
650 $aCaprino
650 $aCriopreservação
650 $aSêmen
653 $aGlicerol
653 $aGOT
700 1 $aHOLTZ, W.
773 $tTheriogenology$gv.42, n.3, p.547-555, 1994.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
