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Registro Completo |
Biblioteca(s): |
Embrapa Acre; Embrapa Amazônia Oriental; Embrapa Gado de Corte. |
Data corrente: |
05/09/2024 |
Data da última atualização: |
25/11/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PANTOJA, K. F. C.; BOARI, A. de J.; MARCHI, B. R. de; REZENDE, J. A. M.; KITAJIMA, E. W.; GONCALVES, R. C.; ASSIS, G. M. L. de; KRAUSE-SAKATE, R. |
Afiliação: |
KÉSSIA FÁTIMA CUNHA PANTOJA, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO"; ALESSANDRA DE JESUS BOARI, CPATU; BRUNO ROSSITTO DE MARCHI, UNIVERSITY OF FLORIDA; JORGE ALBERTO MARQUES REZENDE, UNIVERSIDADE DE SÃO PAULO; ELLIOT WATANABE KITAJIMA, UNIVERSIDADE DE SÃO PAULO; RIVADALVE COELHO GONCALVES, CPAF-AC; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; RENATE KRAUSE-SAKATE, UNIVERSIDADE ESTADUAL PAULISTA "JÚLIO DE MESQUITA FILHO". |
Título: |
Detection and characterization of the cowpea chlorotic mottle virus in forage peanut (Arachis pintoi) in Brazil. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Australasian Plant Pathology, v. 53, n. 5, p. 385-389, Sept. 2024. |
DOI: |
https://doi.org/10.1007/s13313-024-00987-3 |
Idioma: |
Inglês |
Conteúdo: |
The cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (Arachis pintoi), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected A. pintoi leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic A. pintoi plants resulted in systemic mosaic in A. pintoi cv. BRS Mandobi, Vigna unguiculata L. cv Pretinha, and V. unguiculata sg. sesquipedalis cv. De Metro plants; mottle on leaves of inoculated Nicotiana occidentalis and N. benthamiana; and local necrotic and chlorotic lesions in Chenopodium quinoa and C. amaranticolor, respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected A. pintoi samples. To our knowledge, this is the first report of CCMV infecting forage peanuts. |
Palavras-Chave: |
Amendoim forrageiro; CCMV. |
Thesagro: |
Amendoim; Doença de Planta; Vírus. |
Thesaurus Nal: |
Arachis pintoi; Bromovirus. |
Categoria do assunto: |
-- H Saúde e Patologia |
Marc: |
LEADER 02171naa a2200301 a 4500 001 2167157 005 2024-11-25 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s13313-024-00987-3$2DOI 100 1 $aPANTOJA, K. F. C. 245 $aDetection and characterization of the cowpea chlorotic mottle virus in forage peanut (Arachis pintoi) in Brazil.$h[electronic resource] 260 $c2024 520 $aThe cowpea chlorotic mottle virus (CCMV) was detected in forage peanut plants (Arachis pintoi), showing mosaic, yellowing, and interveinal chlorosis. Negatively stained partially purified preparations contained isometric particles ca. 30 nm in size, also present in the cytoplasm and vacuole of leaf parenchymal cells from CCMV-infected A. pintoi leaves. Mechanical transmission of the CCMV leaf extracts from symptomatic A. pintoi plants resulted in systemic mosaic in A. pintoi cv. BRS Mandobi, Vigna unguiculata L. cv Pretinha, and V. unguiculata sg. sesquipedalis cv. De Metro plants; mottle on leaves of inoculated Nicotiana occidentalis and N. benthamiana; and local necrotic and chlorotic lesions in Chenopodium quinoa and C. amaranticolor, respectively. High-throughput sequencing (HTS) allowed us to identify the causal agent as the CCMV and obtain the complete nucleotide sequence of the three genome components. BLAST search in the nucleotide database revealed high identities of the genome components with previously reported CCMV isolates. Primer was designed based on the HTS-derived sequence and efficiently detected CCMV in the infected A. pintoi samples. To our knowledge, this is the first report of CCMV infecting forage peanuts. 650 $aArachis pintoi 650 $aBromovirus 650 $aAmendoim 650 $aDoença de Planta 650 $aVírus 653 $aAmendoim forrageiro 653 $aCCMV 700 1 $aBOARI, A. de J. 700 1 $aMARCHI, B. R. de 700 1 $aREZENDE, J. A. M. 700 1 $aKITAJIMA, E. W. 700 1 $aGONCALVES, R. C. 700 1 $aASSIS, G. M. L. de 700 1 $aKRAUSE-SAKATE, R. 773 $tAustralasian Plant Pathology$gv. 53, n. 5, p. 385-389, Sept. 2024.
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1. |  | OLIVEIRA, C. de S.; VIEIRA, I. M. S.; CONCEICAO, H. E. O. da; SOARES, M. C. L.; MONTEIRO, A. L. C. Propagação e cultivo in vitro de copaíba (Copaifera multijuga, Hayne). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA FCAP, 12.; SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 6., 2002, Belém, PA. A contribuição do profissional de Ciências Agrárias no uso e conservação da biodiversidade: anais. Belém, PA: FCAP: Embrapa Amazônia Oriental, 2002.Tipo: Resumo em Anais de Congresso |
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