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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
10/09/2025 |
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Data da última atualização: |
16/09/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALMEIDA, J. V. F. C. de; FACCIOLI-MARTINS, P. Y.; FERRANTE, M.; MONTEIRO, J. P.; PINHEIRO, R. R.; LIMA, A. M. C.; GERN, J. C.; MOSQUEIRA, V. C. F.; IBARRA, M.; LOURES, M. D. de A.; GUIMARÃES, I. B.; ONORATO, G. de C.; GUIMARAES, A. de S.; BRANDAO, H. de M. |
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Afiliação: |
JOAO VITOR FERNANDES COTRIM DE ALMEIDA, FEDERAL UNIVERSITY OF LAVRAS; PATRICIA YOSHIDA FACCIOLI MARTINS, CNPC; MARCOS FERRANTE, FEDERAL UNIVERSITY OF LAVRAS; JOMAR PATRICIO MONTEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; ANA MILENA CESAR LIMA; JULIANA CARINE GERN, CNPGL; VANESSA CARLA FURTADO MOSQUEIRA, FEDERAL UNIVERSITY OF OURO PRETO; MANUEL IBARRA; MICHELLE DAIANE DE ALMEIDA LOURES, CNPGL; ISABELLA BITENCOURT GUIMARÃES; GEOVANA DE CARVALHO ONORATO; ALESSANDRO DE SA GUIMARAES; HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
In vitro infection model in primary macrophages and in vivo evaluation of benzathine cloxacillin nanoparticles for the treatment of Corynebacterium pseudotuberculosis in goats. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 251, e107561, Oct. 2025. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2025.107561 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this study was to evaluate the in vitro effect of cloxacillin-loaded polymeric nanoparticles (CLXNPs) on C. pseudotuberculosis and to determine the pharmacokinetics of cloxacillin after subcutaneous (SC) administration in goats naturally infected with C. pseudotuberculosis. An in vitro phagocytosis assay with ovine primary macrophages was standardized to evaluate the delivery efficiency and therapeutic effect of CLXNPs. A single SC dose of 1.81 mg/kg of CLXNPs was administered to eight Toggenburg female goats with abscesses caused by C. pseudotuberculosis. Plasma and abscess fluid samples were collected for 48 h following administration. The CLXNPs exhibited a size of 188.41 ± 49.34 nm, a polydispersity index of 0.18 ± 0.07 and a zeta potential of - 33.93 ± 6.42 mV. In the in vitro assay, 8 µg/mL CLXNPs reduced the bacterial count by 99.90 % (equivalent to at least a 5-log reduction - bactericidal effect) in both intracellular and extracellular compartments. The mean maximum plasma concentration was 16.35 ± 12.88 ng/mL, while that in the abscess fluid was 17.45 ± 10.88 ng/mL. The mean apparent elimination half-life of CLXNPs in blood plasma was 2.01 ± 0.65 h. The ability to increase the duration of time that CLX remains in macrophages, as well as the intracellular CLXNPs activity against C. pseudotuberculosis, was successfully demonstrated. These preliminary data can serve as a guide for a larger clinical study in goats infected with C. pseudotuberculosis to define both a dose and a therapeutic regimen for the treatment of caseous lymphadenitis with a novel formulation of CLXNPs. MenosAbstract: The aim of this study was to evaluate the in vitro effect of cloxacillin-loaded polymeric nanoparticles (CLXNPs) on C. pseudotuberculosis and to determine the pharmacokinetics of cloxacillin after subcutaneous (SC) administration in goats naturally infected with C. pseudotuberculosis. An in vitro phagocytosis assay with ovine primary macrophages was standardized to evaluate the delivery efficiency and therapeutic effect of CLXNPs. A single SC dose of 1.81 mg/kg of CLXNPs was administered to eight Toggenburg female goats with abscesses caused by C. pseudotuberculosis. Plasma and abscess fluid samples were collected for 48 h following administration. The CLXNPs exhibited a size of 188.41 ± 49.34 nm, a polydispersity index of 0.18 ± 0.07 and a zeta potential of - 33.93 ± 6.42 mV. In the in vitro assay, 8 µg/mL CLXNPs reduced the bacterial count by 99.90 % (equivalent to at least a 5-log reduction - bactericidal effect) in both intracellular and extracellular compartments. The mean maximum plasma concentration was 16.35 ± 12.88 ng/mL, while that in the abscess fluid was 17.45 ± 10.88 ng/mL. The mean apparent elimination half-life of CLXNPs in blood plasma was 2.01 ± 0.65 h. The ability to increase the duration of time that CLX remains in macrophages, as well as the intracellular CLXNPs activity against C. pseudotuberculosis, was successfully demonstrated. These preliminary data can serve as a guide for a larger clinical study in goats infected with C. pseudotuberculosi... Mostrar Tudo |
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Palavras-Chave: |
Bacterial diseases; Doença infecciosa. |
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Thesagro: |
Bactéria; Caprino; Corynebacterium Pseudotuberculosis; Doença Animal; Linfadenite Caseosa; Sanidade Animal. |
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Thesaurus Nal: |
Antibiotics; Caseous lymphadenitis; Drug delivery systems; Goats; Infectious diseases; Nanospheres; Phagocytosis. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03150naa a2200469 a 4500
001 2178694
005 2025-09-16
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2025.107561$2DOI
100 1 $aALMEIDA, J. V. F. C. de
245 $aIn vitro infection model in primary macrophages and in vivo evaluation of benzathine cloxacillin nanoparticles for the treatment of Corynebacterium pseudotuberculosis in goats.$h[electronic resource]
260 $c2025
520 $aAbstract: The aim of this study was to evaluate the in vitro effect of cloxacillin-loaded polymeric nanoparticles (CLXNPs) on C. pseudotuberculosis and to determine the pharmacokinetics of cloxacillin after subcutaneous (SC) administration in goats naturally infected with C. pseudotuberculosis. An in vitro phagocytosis assay with ovine primary macrophages was standardized to evaluate the delivery efficiency and therapeutic effect of CLXNPs. A single SC dose of 1.81 mg/kg of CLXNPs was administered to eight Toggenburg female goats with abscesses caused by C. pseudotuberculosis. Plasma and abscess fluid samples were collected for 48 h following administration. The CLXNPs exhibited a size of 188.41 ± 49.34 nm, a polydispersity index of 0.18 ± 0.07 and a zeta potential of - 33.93 ± 6.42 mV. In the in vitro assay, 8 µg/mL CLXNPs reduced the bacterial count by 99.90 % (equivalent to at least a 5-log reduction - bactericidal effect) in both intracellular and extracellular compartments. The mean maximum plasma concentration was 16.35 ± 12.88 ng/mL, while that in the abscess fluid was 17.45 ± 10.88 ng/mL. The mean apparent elimination half-life of CLXNPs in blood plasma was 2.01 ± 0.65 h. The ability to increase the duration of time that CLX remains in macrophages, as well as the intracellular CLXNPs activity against C. pseudotuberculosis, was successfully demonstrated. These preliminary data can serve as a guide for a larger clinical study in goats infected with C. pseudotuberculosis to define both a dose and a therapeutic regimen for the treatment of caseous lymphadenitis with a novel formulation of CLXNPs.
650 $aAntibiotics
650 $aCaseous lymphadenitis
650 $aDrug delivery systems
650 $aGoats
650 $aInfectious diseases
650 $aNanospheres
650 $aPhagocytosis
650 $aBactéria
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença Animal
650 $aLinfadenite Caseosa
650 $aSanidade Animal
653 $aBacterial diseases
653 $aDoença infecciosa
700 1 $aFACCIOLI-MARTINS, P. Y.
700 1 $aFERRANTE, M.
700 1 $aMONTEIRO, J. P.
700 1 $aPINHEIRO, R. R.
700 1 $aLIMA, A. M. C.
700 1 $aGERN, J. C.
700 1 $aMOSQUEIRA, V. C. F.
700 1 $aIBARRA, M.
700 1 $aLOURES, M. D. de A.
700 1 $aGUIMARÃES, I. B.
700 1 $aONORATO, G. de C.
700 1 $aGUIMARAES, A. de S.
700 1 $aBRANDAO, H. de M.
773 $tSmall Ruminant Research$gv. 251, e107561, Oct. 2025.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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