| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
30/11/2021 |
|
Data da última atualização: |
30/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
GUERRA, P. A.; SOUZA, E. H. de; ANDRADE, E. C. de; MAX, D. de A. S.; OLIVEIRA, R. S.; SOUZA, F. V. D. |
|
Afiliação: |
PATRÍCIA ARAÚJO GUERRA, UFRB; EVERTON HILO DE SOUZA, UFRB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; DANIELA DE ANDRADE SILVA MAX, UFRB; RAFAELLE SOUZA DE OLIVEIRA, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
|
Título: |
Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, 2020. |
|
ISSN: |
1475-2689 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed with virus-free plants. MenosThe Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus(AGB-009), var. bracteatus(AGB-119), and var. parguazensis(AGB376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal exposure time was 60 min, with plant regeneration from nearly 80% of the shoot tips. For the accessions AGB-009 and AGB-376, all the recovered plants were virus free by the two methods, while 50% of the plants from accession AGB-119 remained infected. These results indicate that shoot tip culture alone or in association with cryotherapy is a promising routine method for virus removal from pineapple plant tissues and is useful to ensure that backup reserves of pineapple germplasm, conserved by in vitro bank and cryobank, are formed wi... Mostrar Tudo |
|
Thesagro: |
Abacaxi; Planta Ornamental. |
|
Categoria do assunto: |
-- |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | SIQUEIRA, K. M. M.; RIBEIRO, M. de F.; KIILL, L. H. P.; COELHO, M. S.; ARAUJO, D. C. S.; GAMA, D. R. S.; LIMA, JÚNIOR, I. O. Effect of agrochemicals on the pattern of visitation of honey bees (Apis mellifera) in melon (Cucumis melo) flowers in Brazilian Northeast. Julius Kühn Archiv, n. 437, p. 180-183, 2012. Edição dos Proceedings of the 11 International Symposium Hazards of Pesticides to Bees, Wageningen, nov. 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
|    |
| 2. | | COELHO, M. S.; LIMA JÚNIOR, I. O.; SILVA, T. A.; SILVA, N. B. G.; SANTOS, A. P. B.; BISPO, L. P.; KIILL, L. H. P.; SIQUEIRA, K. M. M. Comparação da visitação de Apis mellifera em flores de meloeiro em duas estações do ano, na região de Juazeiro-BA. Mensagem Doce, São Paulo, n. 116, p. 65, maio, 2012. Edição dos Resumos do 5 Congresso Brasileiro de Meliponicultura e 19 Congresso Brasileiro de Apicultura, Gramado, maio 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
