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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/07/2023 |
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Data da última atualização: |
10/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HIMMEN, F. D. A.; SOUZA, F. A. de; SILVA-CARDOSO, I. M. de A.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
FERNANDA DUARTE ARAÚJO HIMMEN, UNIVERSIDADE DE BRASILIA; FRANCISCO ADRIANO DE SOUZA, CNPMS; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; ANDRE LUIS XAVIER DE SOUZA, Cenargen; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
Micropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 155, p. 41-56, 2023. |
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DOI: |
https://doi.org/10.1007/s11240-023-02547-x |
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Idioma: |
Inglês |
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Conteúdo: |
Considering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets. MenosConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of th... Mostrar Tudo |
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Palavras-Chave: |
Arbuscular mycorrhizal fungi; Bambusoideae; Epigenética; Fungo micorrízico arbuscular; In vitro propagation; Propagação in vitro. |
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Thesagro: |
Bambu; DNA; Fungo; Micorriza; Semente. |
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Thesaurus Nal: |
Bamboos; Epigenetics. |
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Categoria do assunto: |
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G Melhoramento Genético |
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Marc: |
LEADER 02599naa a2200337 a 4500
001 2154816
005 2024-04-10
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-023-02547-x$2DOI
100 1 $aHIMMEN, F. D. A.
245 $aMicropropagation, estimation of DNA methylation during multiplication cycles and mycorrhization of seed-derived Dendrocalamus asper (Schultes f.) Backer ex Heyne.$h[electronic resource]
260 $c2023
520 $aConsidering the economic significance of Dendrocalamus asper, a woody bamboo species, the objective of this study was to establish an in vitro micropropagation and mycorrhization protocol, and to analyze the DNA methylation dynamics during the multiplication stage of plants of this species. For shoot proliferation, meta-topolin (mT), 6-benzylaminopurine (BAP) and kinetin (Kin) were evaluated at different concentrations (0, 3.3, 6.6 and 13.3 µM) during three monthly subcultures. For in vitro mycorrhization, Rhizoglomus clarum inoculated in liquid medium was used. Murashige and Skoog (MS) and Strullu and Romand (MSR) culture media were tested with different modifications, such as reduced phosphorus, sucrose, and salts. The evidence of root colonization (spores, hyphae, or vesicles) was assessed. For micropropagation, it was verified that mT was as efficient as BAP for the in vitro multiplication of D. asper shoots. There was also a substantial reduction in the number of shoots in the third subculture, which coincided with a significant decrease in DNA methylation evaluated via ELISA, unprecedented analysis carried out during bamboo micropropagation. As for mycorrhization, colonization was observed only in plants grown on MSR (16% of the plants) and MS/2 medium with 25% of the total amount of sucrose (25% of the plants). These results will support the development of efficient in vitro mycorrhization protocols for D. asper and, consequently, the optimization of the quality of the micropropagated plantlets.
650 $aBamboos
650 $aEpigenetics
650 $aBambu
650 $aDNA
650 $aFungo
650 $aMicorriza
650 $aSemente
653 $aArbuscular mycorrhizal fungi
653 $aBambusoideae
653 $aEpigenética
653 $aFungo micorrízico arbuscular
653 $aIn vitro propagation
653 $aPropagação in vitro
700 1 $aSOUZA, F. A. de
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tPlant Cell, Tissue and Organ Culture$gv. 155, p. 41-56, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Agroindústria Tropical; Embrapa Arroz e Feijão; Embrapa Clima Temperado; Embrapa Florestas; Embrapa Gado de Leite; Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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Data corrente: |
27/04/2018 |
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Data da última atualização: |
10/10/2018 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
CUADRA, S. V.; HEINEMANN, A. B.; MADARI, B. E.; ASSAD, E. D.; OLIVEIRA, P. P. A.; ANGELOTTI, F.; GIONGO, V.; VICTORIA, D. de C.; PEREIRA, L. G. R.; GONDIM, R. S.; OLIVEIRA, A. F. de; HIGA, R. C. V. |
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Afiliação: |
SANTIAGO VIANNA CUADRA, CPACT; ALEXANDRE BRYAN HEINEMANN, CNPAF; BEATA EMOKE MADARI, CNPAF; EDUARDO DELGADO ASSAD, CNPTIA; PATRICIA PERONDI ANCHAO OLIVEIRA, CPPSE; FRANCISLENE ANGELOTTI, CPATSA; VANDERLISE GIONGO, CPATSA; DANIEL DE CASTRO VICTORIA, CNPTIA; LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL; RUBENS SONSOL GONDIM, CNPAT; ARYEVERTON FORTES DE OLIVEIRA, CNPTIA; ROSANA CLARA VICTORIA HIGA, CNPF. |
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Título: |
Mudanças climáticas e a agropecuária brasileira. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: CUADRA, S. V.; HEINEMANN, A. B.; BARIONI, L. G.; MOZZER, G. B.; BERGIER, I. (Ed.). Ação contra a mudança global do clima: contribuições da Embrapa. Brasília, DF: Embrapa, 2018. |
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Páginas: |
Cap. 2. |
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Descrição Física: |
E-book. |
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ISBN: |
978-85-7035-793-9 |
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Idioma: |
Português |
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Conteúdo: |
Os agroecossistemas do País terão que simultaneamente buscar inovações tecnológicas que permitam a mitigação das emissões de GEE e a adaptação às alterações climáticas, de modo a garantir no médio e longo prazos a produção de alimentos à sua população atual e futura, bem como a geração de divisas por meio das exportações de commodities e, especialmente, de produtos de maior valor agregado da agroindústria. |
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Palavras-Chave: |
Agropecuária brasileira; Emissão de gases; Mudanças climáticas; Objetivo de desenvolvimento sustentável; Selo ODS 13; Sustentabilidade. |
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Thesagro: |
Agricultura; Clima; Ecossistema; Efeito Estufa; Mudança Climática; Políticas Públicas; Temperatura. |
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Thesaurus NAL: |
Climate; Climate change; Global warming; Greenhouse gas emissions; Public policy. |
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Categoria do assunto: |
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P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184190/1/Livro-Santiago-Cuadra-23-32.pdf
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Marc: |
LEADER 01931naa a2200493 a 4500
001 2097197
005 2018-10-10
008 2018 bl uuuu u00u1 u #d
020 $a978-85-7035-793-9
100 1 $aCUADRA, S. V.
245 $aMudanças climáticas e a agropecuária brasileira.$h[electronic resource]
260 $c2018
300 $aCap. 2.$cE-book.
520 $aOs agroecossistemas do País terão que simultaneamente buscar inovações tecnológicas que permitam a mitigação das emissões de GEE e a adaptação às alterações climáticas, de modo a garantir no médio e longo prazos a produção de alimentos à sua população atual e futura, bem como a geração de divisas por meio das exportações de commodities e, especialmente, de produtos de maior valor agregado da agroindústria.
650 $aClimate
650 $aClimate change
650 $aGlobal warming
650 $aGreenhouse gas emissions
650 $aPublic policy
650 $aAgricultura
650 $aClima
650 $aEcossistema
650 $aEfeito Estufa
650 $aMudança Climática
650 $aPolíticas Públicas
650 $aTemperatura
653 $aAgropecuária brasileira
653 $aEmissão de gases
653 $aMudanças climáticas
653 $aObjetivo de desenvolvimento sustentável
653 $aSelo ODS 13
653 $aSustentabilidade
700 1 $aHEINEMANN, A. B.
700 1 $aMADARI, B. E.
700 1 $aASSAD, E. D.
700 1 $aOLIVEIRA, P. P. A.
700 1 $aANGELOTTI, F.
700 1 $aGIONGO, V.
700 1 $aVICTORIA, D. de C.
700 1 $aPEREIRA, L. G. R.
700 1 $aGONDIM, R. S.
700 1 $aOLIVEIRA, A. F. de
700 1 $aHIGA, R. C. V.
773 $tIn: CUADRA, S. V.; HEINEMANN, A. B.; BARIONI, L. G.; MOZZER, G. B.; BERGIER, I. (Ed.). Ação contra a mudança global do clima: contribuições da Embrapa. Brasília, DF: Embrapa, 2018.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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