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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/10/2018 |
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Data da última atualização: |
14/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATEUS, J. R.; MARQUES, J. M.; DAL RIO, I.; VOLLÚ, R. E.; COELHO, M. R. R.; SELDIN, L. C. |
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Afiliação: |
UFRJ; UFPA; UFRJ; UFRJ; MARCIA REED RODRIGUES COELHO, CNPAB; UFRJ. |
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Título: |
Response of the microbial community associated with sweet potato (Ipomoea batatas) to Bacillus safensis and Bacillus velezensis strains. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoek, v. 112, n. 4, p. 501-512. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-018-1181-y |
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Idioma: |
Inglês |
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Conteúdo: |
Sweet potato is a subsistence crop cultivated worldwide. Although it is generally considered tolerant to different diseases, it is quite susceptible to the fungus Plenodomus destruens that causes foot-rot disease. Plant growth-promoting bacteria associated with sweet potato remain poorly studied, but some Bacillus strains may have potential as biological control agents. Here, we evaluate the persistence of two bacterial strains?Bacillus safensis T052-76 and Bacillus velezensis T149-19?in pot experiments and assess their impact on indigenous bacterial and fungal communities associated with sweet potato. Numbers of cells of both strains introduced into pots remained stable in the rhizosphere of sweet potato over the 180-day experiment. Denaturing gradient gel electrophoresis based on the rrs gene encoding bacterial 16S rRNA and the fungal ribosomal internal transcribed spacer region showed that bands corresponding to the introduced strains were not detected in plant endosphere. PERMANOVA and non-metric multidimensional scaling statistical analyses showed that: (1) strain T052-76 altered the structure of the indigenous bacterial community (rhizosphere and soil) more than strain T149-19; (2) T052-76 slightly altered the structure of the indigenous fungal community (rhizosphere and soil) and (3) strain T149-19 did not disturb the fungal community. Our results demonstrate the stability of both Bacillus strains in the sweet potato rhizosphere and, apart from the influence of B. safensis T052-76 on the bacterial community, their limited impact on the microbial community associated with this important crop plant. MenosSweet potato is a subsistence crop cultivated worldwide. Although it is generally considered tolerant to different diseases, it is quite susceptible to the fungus Plenodomus destruens that causes foot-rot disease. Plant growth-promoting bacteria associated with sweet potato remain poorly studied, but some Bacillus strains may have potential as biological control agents. Here, we evaluate the persistence of two bacterial strains?Bacillus safensis T052-76 and Bacillus velezensis T149-19?in pot experiments and assess their impact on indigenous bacterial and fungal communities associated with sweet potato. Numbers of cells of both strains introduced into pots remained stable in the rhizosphere of sweet potato over the 180-day experiment. Denaturing gradient gel electrophoresis based on the rrs gene encoding bacterial 16S rRNA and the fungal ribosomal internal transcribed spacer region showed that bands corresponding to the introduced strains were not detected in plant endosphere. PERMANOVA and non-metric multidimensional scaling statistical analyses showed that: (1) strain T052-76 altered the structure of the indigenous bacterial community (rhizosphere and soil) more than strain T149-19; (2) T052-76 slightly altered the structure of the indigenous fungal community (rhizosphere and soil) and (3) strain T149-19 did not disturb the fungal community. Our results demonstrate the stability of both Bacillus strains in the sweet potato rhizosphere and, apart from the influence of B. saf... Mostrar Tudo |
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Palavras-Chave: |
Bacillus safensis; Bacillus velezensis; Microbial community. |
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Thesaurus Nal: |
Sweet potatoes. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02442naa a2200253 a 4500
001 2097752
005 2019-11-14
008 2019 bl uuuu u00u1 u #d
022 $a1572-9699
024 7 $ahttps://doi.org/10.1007/s10482-018-1181-y$2DOI
100 1 $aMATEUS, J. R.
245 $aResponse of the microbial community associated with sweet potato (Ipomoea batatas) to Bacillus safensis and Bacillus velezensis strains.$h[electronic resource]
260 $c2019
520 $aSweet potato is a subsistence crop cultivated worldwide. Although it is generally considered tolerant to different diseases, it is quite susceptible to the fungus Plenodomus destruens that causes foot-rot disease. Plant growth-promoting bacteria associated with sweet potato remain poorly studied, but some Bacillus strains may have potential as biological control agents. Here, we evaluate the persistence of two bacterial strains?Bacillus safensis T052-76 and Bacillus velezensis T149-19?in pot experiments and assess their impact on indigenous bacterial and fungal communities associated with sweet potato. Numbers of cells of both strains introduced into pots remained stable in the rhizosphere of sweet potato over the 180-day experiment. Denaturing gradient gel electrophoresis based on the rrs gene encoding bacterial 16S rRNA and the fungal ribosomal internal transcribed spacer region showed that bands corresponding to the introduced strains were not detected in plant endosphere. PERMANOVA and non-metric multidimensional scaling statistical analyses showed that: (1) strain T052-76 altered the structure of the indigenous bacterial community (rhizosphere and soil) more than strain T149-19; (2) T052-76 slightly altered the structure of the indigenous fungal community (rhizosphere and soil) and (3) strain T149-19 did not disturb the fungal community. Our results demonstrate the stability of both Bacillus strains in the sweet potato rhizosphere and, apart from the influence of B. safensis T052-76 on the bacterial community, their limited impact on the microbial community associated with this important crop plant.
650 $aSweet potatoes
653 $aBacillus safensis
653 $aBacillus velezensis
653 $aMicrobial community
700 1 $aMARQUES, J. M.
700 1 $aDAL RIO, I.
700 1 $aVOLLÚ, R. E.
700 1 $aCOELHO, M. R. R.
700 1 $aSELDIN, L. C.
773 $tAntonie van Leeuwenhoek$gv. 112, n. 4, p. 501-512.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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