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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2018 |
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Data da última atualização: |
02/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WOHLRES-VIANA, S.; ARASHIRO, E. K. N.; MINARE, T. P.; FERNANDES, C. A. C.; GRAZIA, J. G. V.; SIQUEIRA, L. G. B.; MACHADO, M. A.; VIANA, J. H. M. |
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Afiliação: |
UNIVERSIDADE FEDERAL DE JUIZ DE FORA; UNIVERSIDADE FEDERAL FLUMINENSE; UNIVERSIDADE JOSÉ DO ROSARIO VELLANO; UNIVERSIDADE JOSE DO ROSARIO VELLANO; UNIVERSIDADE FEDERAL DE MINAS GERAIS; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; JOAO HENRIQUE MOREIRA VIANA, CENARGEN. |
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Título: |
Differential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Theriogenology, v. 126, p. 68-74, 2019. |
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DOI: |
10.1016/j.theriogenology.2018.12.004 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype. MenosAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0... Mostrar Tudo |
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Palavras-Chave: |
In vivo embryo production. |
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Thesaurus Nal: |
Embryo transfer; Luteinizing hormone. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02857naa a2200253 a 4500
001 2106166
005 2024-07-02
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.theriogenology.2018.12.004$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aDifferential expression of LHCGR and its isoforms is associated to the variability in superovulation responses of Gir cattle.$h[electronic resource]
260 $c2019
520 $aAbstract The aim of this study was to evaluate the pattern of expression of LHCGR isoforms in Gir heifers characterized as good (10.3 ± 1.2 ova/embryos per flush, n = 5) or poor responders (1.1 ± 0.3 ova/embryos per flush, n = 5) to superovulation protocols. In both groups, an adapted ultrasound-guided follicular aspiration system was used to collect granulosa cells from 8 mm follicles formed either during a synchronized, non-stimulated follicular wave (no stimulation control, NS) or on the fourth day of a superovulation protocol (SOV) induced with 200 IU of pFSH. The recovered follicular fluid was centrifuged and granulosa cells were washed with NaCl 0.9% and kept in RNAlater®. RNA extraction was performed using a commercial RNeasy Micro Kit and eluted samples were quantified and reverse transcribed using the commercial Superscript III kit. cDNA samples were amplified by real-time PCR using a primer to target LH/hCG receptor gene - not selective for LHCGR isoforms (total LHCGR) - and four sets of isoforms selective primers (S1, S10, S10 + 11, and S11). Analyses were performed using the REST software and expression levels are shown as mean ± SEM. Under physiological conditions (NS), poor responders had a higher expression of total LHCGR (4.9 ± 1.7 fold-change, P < 0.01) as well as isoforms S10, S11 and S10 + 11, compared to good responders. In both phenotypes, superovulation down-regulated total LHCGR expression (-0.5 ± 0.2 and -0.9 ± 0.0 for good and poor responders, respectively; P < 0.05). However, in poor responders the exogenous FSH treatment up-regulated the S10 (2.4 ± 2.0; P < 0.05), S10 + 11 (3.8 ± 3.2; P < 0.01), and S1 isoforms (1.8 ± 1.3; P < 0.05), compared to good responders We conclude that down-regulation of total LHCGR, associated to up-regulation of their inactive isoforms, may have compromised follicle development and thus contributed to the low efficiency of superovulation in heifers with a poor responder phenotype.
650 $aEmbryo transfer
650 $aLuteinizing hormone
653 $aIn vivo embryo production
700 1 $aARASHIRO, E. K. N.
700 1 $aMINARE, T. P.
700 1 $aFERNANDES, C. A. C.
700 1 $aGRAZIA, J. G. V.
700 1 $aSIQUEIRA, L. G. B.
700 1 $aMACHADO, M. A.
700 1 $aVIANA, J. H. M.
773 $tTheriogenology$gv. 126, p. 68-74, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 11 | |
| 6. |  | BARDALES, N. G.; LIMA, M. V. de O.; MAIA, G. R.; BEZERRA, D. C. F.; AMARAL, E. F. do; ARAÚJO, E. A.; AMARAL, E. F. do. Avaliação da adubação fosfatada no crescimento radicular da Sena siamea cultivada em aléas no estado do Acre. In: REUNIÃO ANUAL DA SBPC, 53., 2001, Salvador. Nação e diversidade: patrimônio do futuro: anais/resumos. Salvador: SBPC, 2001. 1 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 7. | | RAMOS, A. F.; SALMAN, A. K. D.; RÊGO, A. C. do; FATURI, C.; MAIA, G. F. N.; CAVALI, J.; PORTO, M. O.; SALES, M. F. L. Alimentos e alimentação. In: SALMAN, A. K. D.; PFEIFER, L. F. M. (Ed.). Pecuária leiteira na Amazônia. Brasília, DF: Embrapa, 2020. Cap. 13, p. 281-317.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Acre; Embrapa Rondônia. |
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| 8. | | SALES, M. F. L.; ROSA, B. L.; PALU, A. B.; MAIA, G. F. N.; MIRANDA, L. A.; PEIXOTO, R. M.; DINIZ, J. V. A. Productive performance and economic viability of beef cattle subjected to different castration methods in the Western Amazon. Livestock Science, v. 251, 104633, Sept. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Acre. |
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| 9. | | DINIZ, J. V. A.; DELL'AQUA JUNIOR, J. A.; OBA, E.; FREITAS, R. R. de; PEIXOTO, R. M.; SILVA, L. O.; MAIA, G. F. N. M.; CARVALHO, B. P.; LOUREIRO, B.; SATRAPA, R. A. Influência da progesterona injetável na taxa de prenhez e perda gestacional de fêmeas nelore de distintas categorias reprodutivas submetidas a inseminação artificial em tempo fixo. Acta Scientiae Veterinariae, v. 49, 1828, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Acre. |
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| 10. | | COSTA, F. de S.; CAMPOS FILHO, M. D.; SANTIAGO, A. C. C.; MAGALHÃES, I. B.; CORDEIRO, L. da S.; LIMA, A. P. de; MAIA, G. R.; SILVA, E. P.; KLEIN, M. A.; SILVA, F. de A. C.; BARDALES, N. G.; QUEIROZ, L. R.; BRITO, E. de S. Agricultura conservacionista na produção familiar de mandioca e milho no Juruá, Estado do Acre: efeitos da adoção nos resultados de safras de 2006 a 2014. Rio Branco, AC: Embrapa Acre, 2014. 10 p. (Embrapa Acre. Comunicado técnico, 186).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Acre. |
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| 11. | | BARDALES, N. G.; ARAÚJO. E. A. de; AMARAL, E. F. do; KER, J. C.; MAIA, G. R.; ARAÚJO, D. R. de; AMARAL, E. F. do; OLIVEIRA, T. K. de; FRANKE, I. L.; LANI, J. L.; MARTORANO, L. G.; MELO, A. W. F. de; NEGREIROS, J. da S. Solos e geopaisagens do Município de Cruzeiro do Sul, Estado do Acre: potencialidades e fragilidades. Cruzeiro do Sul, AC: IPAM Amazônia, 2021. 167 p.| Tipo: Autoria/Organização/Edição de Livros |
| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| Registros recuperados : 11 | |
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