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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/03/2011 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLISSENI, J.; SÁ, W. F. de; GUERRA, M. O.; MACHADO, M. A.; SERAPIÃO, R. V.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; PETERS, V. M. |
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Afiliação: |
JULIANA POLISSENI, UFJF; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; MARTHA DE OLIVEIRA GUERRA, UFJF; MARCO ANTONIO MACHADO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFJF; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
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Título: |
Post biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Fertility and Sterility, v. 93. n. 3, p. 783-788, 2010. |
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DOI: |
https://doi.org/10.1016/j.fertnstert.2008.10.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres. MenosObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell t... Mostrar Tudo |
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Palavras-Chave: |
Embryo quality; Hatching rate; PGD; Sexing. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03686naa a2200265 a 4500
001 1881353
005 2024-02-15
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fertnstert.2008.10.023$2DOI
100 1 $aPOLISSENI, J.
245 $aPost biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis.$h[electronic resource]
260 $c2010
520 $aObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.
653 $aEmbryo quality
653 $aHatching rate
653 $aPGD
653 $aSexing
700 1 $aSÁ, W. F. de
700 1 $aGUERRA, M. O.
700 1 $aMACHADO, M. A.
700 1 $aSERAPIÃO, R. V.
700 1 $aCARVALHO, B. C. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aPETERS, V. M.
773 $tFertility and Sterility$gv. 93. n. 3, p. 783-788, 2010.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 6 | |
| 1. |  | AMARAL, E. F. do; LIMA, M. V. de O.; LUDEWIGS, T.; ANDRADE, A. do C.; BARDALES, N. G.; MENESES FILHO, L. C. de L.; RECCO, R. D.; MELO. A. W. F. de; AMARAL, E. F. do. Distribuição dos sistemas radiculares de ingá-de-macaco (Inga coreacea), Ingá-mirim (Inga fagifolia) e Ingá-de-metro (Inga edulis), cultivados em aléias sobre um argissolo amarelo plíntico no estado do Acre. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 3., 2000, Manaus. Sistemas agroflorestais: manejando a biodiversidade e compondo a paisagem rural: resumos expandidos. Manaus: Embrapa Amazônia Ocidental, 2000. p. 144-146. (Embrapa Amazônia Ocidental. Documentos, 7).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 2. | | OLIVEIRA, P. E. F.; VERZA, N. C.; BITTENCOURT, D.; SOUTO, B. M.; MADEIRA, L. M.; ANDRADE, A. C.; SILVA, F. R. da; LEWIS, R. V.; RECH FILHO, E. L. Producing artificial silk fibers from brazilian spider genes in bacteria and plant systems. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Anais... Águas de Lindóia: SBG, 2007. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 3. | | AMARAL, E. F. do; LIMA, M. V. de O.; LUDEWIGS, T.; ANDRADE, A. do C.; MENESES FILHO, L. C. de L.; RECCO, R. D.; MELO, A. W. F. de; AMARAL, E. F. do. Avaliação do efeito da adubação fosfatada na distribuição do sistema radicular da ingá-de-macaco (Inga coreacea), ingá-mirim (Inga fogifolia) e ingá-de-metro (Inga edulis) cultivadas em aléias no estado do Acre. In: CONGRESSO BRASILEIRO DE SISTEMAS AGROFLORESTAIS, 3., 2000, Manaus. Sistemas agroflorestais: manejando a biodiversidade e compondo a paisagem rural: resumos expandidos. Manaus: Embrapa Amazônia Ocidental, 2000. p. 45-47. (Embrapa Amazônia Ocidental. Documentos, 7).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 4. | | SOUTO, B. M.; VERZA, N. C.; OLIVEIRA, P. E. F.; BITTENCOURT, D. M. de C.; MADEIRA, L. M.; ANDRADE, A. C.; SILVA, F. R. da; LEWIS, R. V.; RECH FILHO, E. L. Produção e purificação de seda recombinante da aranha brasileira Nephilengys cruentata. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Anais... Águas de Lindóia: SBG, 2007. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 5. | | PRIMAVESI, A. C. P. de A.; ANDRADE, A. G. de; ALVES, B. J. R.; ROSSO, C. R. S. de; BATISTA, E. M.; PRATES, H. T.; ORTIZ, F. R.; MELLO, J. C. de; FERRAZ, M. R.; LINHARES, N. W.; MACHADO, P. L. O. de A.; MOELLER, R.; ALVES, R. de C. S.; SILVA, W. M. Água. In: NOGUEIRA, A. R. de A.; SOUZA, G. B. de. (ed.). Manual de laboratórios: solo, água, nutrição vegetal, nutrição animal e alimentos. São Carlos, SP: Embrapa Pecuária Sudeste, 2005. cap. 7, p. 131-142.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Acre. |
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| 6. | | PRIMAVESI, A. C.; ANDRADE, A. G. de; ALVES, B. J. R.; ROSSO, C. R. S. de; BATISTA, E. M.; PRATES, H. T.; ORTIZ, F. R.; MELLO, J.; FERRAZ, M. R.; LINHARES, N. W.; MACHADO, P. L. O. de A.; MOELLER, R.; ALVES, R. de C. S.; SILVA, W. M. Métodos de análise de solo. In: NOGUEIRA, A. R. de A.; SOUZA, G. B. de (ed.). Manual de laboratórios: solo, água, nutrição vegetal, nutrição animal e alimentos. São Carlos, SP: Embrapa Pecuária Sudeste, 2005. p. 63-123.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Acre; Embrapa Amazônia Oriental. |
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| Registros recuperados : 6 | |
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