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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
03/10/2008 |
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Data da última atualização: |
26/06/2023 |
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Autoria: |
VANDER SCHALIE, J.; BRADWAY, D. S.; BESSER, T. S.; EVERMANN, J. F. |
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Título: |
Evaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Journal of Veterinary Diagnostic Investigation, v. 6, n. 1, p. 30-33, Jan. 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
A kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by mmunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples. |
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Palavras-Chave: |
Artrite Encefalite; CAEV; Imunodiagnóstico; Lentivirus Infections; Sulfur Radioisotopes. |
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Thesagro: |
Caprino; Doença Animal. |
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Thesaurus Nal: |
Antibody specificity; Caprine arthritis-encephalitis; Disease diagnosis; Enzyme-linked immunosorbent assay; Goat diseases; Immunology; Kinetics; Methionine; Precipitin tests; Taste sensitivity; Viral antibodies. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02110naa a2200373 a 4500
001 1516073
005 2023-06-26
008 1994 bl uuuu u00u1 u #d
100 1 $aVANDER SCHALIE, J.
245 $aEvaluation of a kinetic enzyme-linked immunosorbent assay for detection of caprine arthritis-encephalitis virus-specific antibodies.$h[electronic resource]
260 $c1994
520 $aA kinetic indirect enzyme-linked immunosorbent assay (k-ELISA) was evaluated for detection of antibody to caprine arthritis-encephalitis virus (CAEV), using sodium dodecyl sulfate-treated CAEV-63 as antigen. Two hundred fifteen caprine sera submitted to the diagnostic laboratory were tested for CAEV antibody by the k-ELISA and by mmunoprecipitation of [35S]-methionine-labeled CAEV. A k-ELISA positive cutoff point of 80 yielded a sensitivity of 94.4% and a specificity of 100%, as compared with immunoprecipitation. A k-ELISA cutoff point of 50 resulted in a sensitivity of 100%, with 95.6% specificity. When sera with k-ELISA scores between 50 and 80 were considered suspect, testing of 1,001 diagnostic sera resulted in < 1.5% suspect reactions. Using the 80 cutoff point, the CAEV k-ELISA had good sensitivity and specificity, with the added advantages of quick turn-around time, few suspect reactions, and adaptability to large numbers of samples.
650 $aAntibody specificity
650 $aCaprine arthritis-encephalitis
650 $aDisease diagnosis
650 $aEnzyme-linked immunosorbent assay
650 $aGoat diseases
650 $aImmunology
650 $aKinetics
650 $aMethionine
650 $aPrecipitin tests
650 $aTaste sensitivity
650 $aViral antibodies
650 $aCaprino
650 $aDoença Animal
653 $aArtrite Encefalite
653 $aCAEV
653 $aImunodiagnóstico
653 $aLentivirus Infections
653 $aSulfur Radioisotopes
700 1 $aBRADWAY, D. S.
700 1 $aBESSER, T. S.
700 1 $aEVERMANN, J. F.
773 $tJournal of Veterinary Diagnostic Investigation$gv. 6, n. 1, p. 30-33, Jan. 1994.
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