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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/12/2017 |
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Data da última atualização: |
04/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ERGUN; SOUZA, F. V. D. |
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Afiliação: |
ERGUN KAYA, Mugla Sitki Kocman University; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In Vitro Cellular & Developmental Biology - Plant, v.53, Issue 4,p 410?417,| August 2017. |
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ISSN: |
1475-2689 |
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DOI: |
10.1007/s11627-017-9837-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Conservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties. MenosConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stab... Mostrar Tudo |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02196naa a2200169 a 4500
001 2081361
005 2017-12-04
008 2017 bl uuuu u00u1 u #d
022 $a1475-2689
024 7 $a10.1007/s11627-017-9837-2$2DOI
100 1 $aERGUN
245 $aComparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers.$h[electronic resource]
260 $c2017
520 $aConservation of Saccharum spp. germplasm as ex situ collections of plants has a high cost, and in natural conditions, the plants remain exposed to pests, pathogens, and natural disasters. Long-term preservation of plant germplasm is important for agricultural biodiversity and food safety, so the aim of this study was to develop a cryogenic procedure for cryopreservation of sugarcane germplasm. The first study compared droplet vitrification and encapsulation-vitrification techniques for cryopreservation of in vitro shoot tips of Saccharum spp. variety Halaii. The best regeneration rate (70.9%) was obtained from 45-min PVS2 vitrification solution-treated shoot tips via the droplet vitrification technique. This technique was tested on two other Saccharum sp. varieties, and the best regeneration rates for varieties NG 57-024 and H 83-6179 were 63.3 and 76.3%, respectively. Shoots derived from cryopreserved shoot tip buds developed well-formed roots, and were easily acclimated to greenhouse conditions. The second study evaluated genetic stability of the cryopreserved varieties using ten inter-simple sequence repeat primers. A total of 211 (Halaii), 198 (H83-6179), and 201 (NG 57-024) reproducible bands, ranging from 125 to 5500 bp, were scored with this technique. One hundred genetic stability was detected from Halaii and H 83-6179 whereas 98.5% genetic stability was detected from varieties of NG 57-024. The PCR reactions showed that there was no crucial variation on genetic stability for all cryopreserved varieties.
650 $aCriopreservação
700 1 $aSOUZA, F. V. D.
773 $tIn Vitro Cellular & Developmental Biology - Plant$gv.53, Issue 4,p 410?417,| August 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 2. |  | WENDLING, B.; JUCKSCH, I.; MENDONÇA, E. de S.; ALMEIDA, R. F. de; ALVARENGA, R. C. Simulação dos estoques de carbono e nitrogênio pelo modelo Century em Latossolos, no cerrado brasileiro. Revista Ciência Agronômica, Fortaleza, v. 45, n. 2, p. 238-248, abr./jun. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | ALBUQUERQUE, C. J. B.; ALVARENGA, R. C.; VIANA, M. C. M.; CLEMENTE, M. A.; WENDLING, B.; ARAUJO, S. A. do C. Cultura do sorgo em sistema Integração Lavoura-Pecuária-Floresta. Informe Agropecuário, Belo Horizonte, v. 35, n. 278, p. 63-73, jan./fev. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
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