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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
17/02/2012 |
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Data da última atualização: |
04/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BATISTA, J. S. da S.; HUNGRIA, M. |
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Afiliação: |
JESIANE STEFÂNIA DA SILVA BATISTA, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomics reveals differential expression of proteins related to a variety of metabolic pathways by genistein-induced Bradyrhizobium japonicum strains. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 75, n. 4, p. 1211-1219, Feb. 2012. |
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DOI: |
10.1016/j.jprot.2011.10.032 |
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Idioma: |
Português |
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Conteúdo: |
The rhizobia?legume symbiosis requires a coordinated molecular interaction between the symbionts, initiated by seed and root exudation of several compounds, mainly flavonoids, that trigger the expression of nodulation genes in the bacteria. Since the role of flavonoids seems to be broader than the induction of nodulation genes, we aimed at characterizing genistein-induced proteins of Bradyrhizobium japonicum CPAC 15 (=SEMIA 5079), used in commercial soybean inoculants in Brazil, and of two genetically related strains grown in vitro. Whole-cell proteins were extracted both from induced (1 ?M genistein) and from non-induced cultures of the three strains, and separated by twodimensional electrophoresis. Spot profiles were compared between the two conditions and selected spots were excised and identified by mass spectrometry. Forty-seven proteins were significantly induced by genistein, including several hypothetical proteins, the cytoplasmic flagellar component FliG, periplasmic ABC transporters, a protein related to biosynthesis of exopolysaccharides (ExoN), and proteins involved in redox-state maintenance. Noteworthy was the induction of the PhyR-?EcfG regulon, recently demonstrated to be involved in the symbiotic efficiency of, and general stress response in B. japonicum. Our results confirm that the role of flavonoids, such as genistein, can go far beyond the expression of nodulation-related proteins in B. japonicum. |
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Thesagro: |
Fixação de Nitrogênio; Simbiose. |
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Thesaurus Nal: |
Nitrogen fixation; Symbiosis. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02110naa a2200193 a 4500
001 1916084
005 2024-11-04
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jprot.2011.10.032$2DOI
100 1 $aBATISTA, J. S. da S.
245 $aProteomics reveals differential expression of proteins related to a variety of metabolic pathways by genistein-induced Bradyrhizobium japonicum strains.$h[electronic resource]
260 $c2012
520 $aThe rhizobia?legume symbiosis requires a coordinated molecular interaction between the symbionts, initiated by seed and root exudation of several compounds, mainly flavonoids, that trigger the expression of nodulation genes in the bacteria. Since the role of flavonoids seems to be broader than the induction of nodulation genes, we aimed at characterizing genistein-induced proteins of Bradyrhizobium japonicum CPAC 15 (=SEMIA 5079), used in commercial soybean inoculants in Brazil, and of two genetically related strains grown in vitro. Whole-cell proteins were extracted both from induced (1 ?M genistein) and from non-induced cultures of the three strains, and separated by twodimensional electrophoresis. Spot profiles were compared between the two conditions and selected spots were excised and identified by mass spectrometry. Forty-seven proteins were significantly induced by genistein, including several hypothetical proteins, the cytoplasmic flagellar component FliG, periplasmic ABC transporters, a protein related to biosynthesis of exopolysaccharides (ExoN), and proteins involved in redox-state maintenance. Noteworthy was the induction of the PhyR-?EcfG regulon, recently demonstrated to be involved in the symbiotic efficiency of, and general stress response in B. japonicum. Our results confirm that the role of flavonoids, such as genistein, can go far beyond the expression of nodulation-related proteins in B. japonicum.
650 $aNitrogen fixation
650 $aSymbiosis
650 $aFixação de Nitrogênio
650 $aSimbiose
700 1 $aHUNGRIA, M.
773 $tJournal of Proteomics$gv. 75, n. 4, p. 1211-1219, Feb. 2012.
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Embrapa Soja (CNPSO) |
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| 2. |  | VILARINHOS, A. D.; BARROS, E. G. de; PAIVA, E.; SEDIYAMA, C. S.; MOREIRA, M. A. Use of the random amplified polymorphic DNA technique to characterize soybean (Glycine max (L.) Merrill) genotypes. Revista Brasileira de Genética, Ribeirão Preto, v. 17, n. 3, p. 287-290, 1994.| Tipo: Artigo em Periódico Indexado |
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