Data/hora: 01/06/2024 06:41:32

Biblioteca(s): | Embrapa Milho e Sorgo.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 1
Data corrente: | 27/10/2010
Data da última atualização: | 04/06/2018
Autoria: | COELHO, G. T. C. P.; CARNEIRO, N. P.; KARTHIKEYAN, A. S.; RAGHOTHAMA, K. G.; SCHAFFERT, R. E.; BRANDAO, R. L.; PAIVA, L. V.; SOUZA, I. R. P. de; ALVES, V. M.; IMOLESI, A.; CARVALHO, C. H. S.; CARNEIRO, A. A.
Afiliação: | Gracielle T. C. P. Coelho, UFLA; NEWTON PORTILHO CARNEIRO, CNPMS; ATHIKKATTUVALASU S. KARTHIKEYAN, Purdue University; KASCHANDRA G. RAGHOTHAMA, Purdue University; ROBERT EUGENE SCHAFFERT, CNPMS; ROSANGELA L. BRANDÃO, UFLA; LUCIANO V. PAIVA, UFLA; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; ANDERSON IMOLESI; CARLOS H. S. CARVALHO; ANDREA ALMEIDA CARNEIRO, CNPMS.
Título: | A phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation.
Ano de publicação: | 2010
Fonte/Imprenta: | Plant Molecular Biology Reporter, Athens, v. 28, p. 717-723, 2010.
DOI: | 10.1007/s1 I 105-010-0199-8
Idioma: | Inglês
Conteúdo: | Phosphorus (P) stress responsive genes have been identified and characterized, including the highaffinity phosphate transporter AtPHTl;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHTl;4 has been fused with the (3-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHTl;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHTl;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHTl;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.
Thesagro: | Fósforo; Genética vegetal.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Milho e Sorgo (CNPMS)
Exemplares: | CNPMS - (23044-1) - UPC - AP - DD -  -  -  - DIS