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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/01/1993 |
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Data da última atualização: |
06/01/1993 |
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Autoria: |
STAEHELIN, C.; MULLER, J.; MELLOR, R. B.; WIEMKEN, A.; BOLLER, T. |
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Afiliação: |
Botanisches Institut der Universitat Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland. |
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Título: |
Chitinase and peroxidase in effective (fix) and ineffective (fix) soybean nodules. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Planta, v.187, p.295-300, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
Chitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nitrogen, and in the entire body of ... MenosChitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nit... Mostrar Tudo |
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Palavras-Chave: |
Chitanase; Glycine; Hypersensitive reaction; Isoenzymes; Nodules. |
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Thesagro: |
Peroxidase. |
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Thesaurus Nal: |
Bradyrhizobium. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02183naa a2200253 a 4500
001 1458238
005 1993-01-06
008 1992 bl --- 0-- u #d
100 1 $aSTAEHELIN, C.
245 $aChitinase and peroxidase in effective (fix) and ineffective (fix) soybean nodules.
260 $c1992
520 $aChitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nitrogen, and in the entire body of ...
650 $aBradyrhizobium
650 $aPeroxidase
653 $aChitanase
653 $aGlycine
653 $aHypersensitive reaction
653 $aIsoenzymes
653 $aNodules
700 1 $aMULLER, J.
700 1 $aMELLOR, R. B.
700 1 $aWIEMKEN, A.
700 1 $aBOLLER, T.
773 $tPlanta$gv.187, p.295-300, 1992.
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Registro original: |
Embrapa Soja (CNPSO) |
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| 1. |  | LIMA, J. A.; ROSSI, A, A. B.; SANTOS, T. de O.; PENNA, G. F.; TARDIN, F. D.; TRINDADE, R. dos S.; GUIMARAES, P. E. de O.; GODINHO, V. de P. C.; AMARAL JUNIOR, A. T. do; CORDEIRO, A. G. M.; SANTOS, R. C. dos; JESUS, M. S. F. de; POGALSKY, L. de S.; TIAGO, A. V.; PEDRI, E. M. de; FERREIRA, E. L.; ZANETTI, G. T. Adaptability and stability of corn hybrids for the south of the Amazon biome via GGE biplot. Pesquisa Agropecuária Brasileira, v. 58, e02931, 2023. Título em português: Adaptabilidade e estabilidade de híbridos de milho para o sul do bioma Amazônia via GGE biplot.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Rondônia; Embrapa Unidades Centrais. |
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