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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
15/07/2010 |
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Data da última atualização: |
18/10/2010 |
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Autoria: |
AMBIEL, A. C.; MACHADO NETO, N. B.; GUABERTO, L. M.; VANDERLEI, T. M. |
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Afiliação: |
ANA CLAUDIA AMBIEL, FCA/UNOESTE; NELSON BARBOSA MACHADO NETO, FCA/UNOESTE; LUCIANA MACHADO GUABERTO, FCA/UNOESTE; TALITA MARQUES VANDERLEI, FCA/UNOESTE. |
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Título: |
Brachiaria germplasm dissimilarity as shown by RAPD markers. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 10, n. 1, p. 55-64, Mar. 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
The main objective of this work was to study the genetic dissimilarity among germplasm accesses of six Brachiaria species and to compare the most important cultivars through the use of RAPD markers with the studied material. DNA was extracted from seeds of five different accesses of B. decumbens, B. ruziziensis, B. nigropedata, B. humidicola, B. jubata and B. brizantha from the EMBRAPA – CNPGC, 12 commercial cultivars, and two accesses each of P. maximum and B. arrecta. From 120 primers tested, 10 polymorphic were selected, amplifying a total of 114 polymorphic bands. Dissimilarity indexes were estimated for all samples analysed, and ranged from 0.262 to 0.907, grouped by UPGMA, and four groups were clearly distinct. All accesses and cultivars of Brachiaria could be grouped through RAPD markers. Dissimilarity within a species was found. The distribution in African species was narrower in B. decumbens and B. ruziziensis showed low levels of dissimilarity, and naturally widely-spread species showed high dissimilarity levels. |
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Palavras-Chave: |
Genetic variability; Marcadores moleculares; Molecular markers; Pastagens; Pasture; Variabilidade genética. |
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Thesagro: |
Brachiaria. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01735naa a2200241 a 4500
001 1857833
005 2010-10-18
008 2010 bl uuuu u00u1 u #d
100 1 $aAMBIEL, A. C.
245 $aBrachiaria germplasm dissimilarity as shown by RAPD markers.
260 $c2010
520 $aThe main objective of this work was to study the genetic dissimilarity among germplasm accesses of six Brachiaria species and to compare the most important cultivars through the use of RAPD markers with the studied material. DNA was extracted from seeds of five different accesses of B. decumbens, B. ruziziensis, B. nigropedata, B. humidicola, B. jubata and B. brizantha from the EMBRAPA – CNPGC, 12 commercial cultivars, and two accesses each of P. maximum and B. arrecta. From 120 primers tested, 10 polymorphic were selected, amplifying a total of 114 polymorphic bands. Dissimilarity indexes were estimated for all samples analysed, and ranged from 0.262 to 0.907, grouped by UPGMA, and four groups were clearly distinct. All accesses and cultivars of Brachiaria could be grouped through RAPD markers. Dissimilarity within a species was found. The distribution in African species was narrower in B. decumbens and B. ruziziensis showed low levels of dissimilarity, and naturally widely-spread species showed high dissimilarity levels.
650 $aBrachiaria
653 $aGenetic variability
653 $aMarcadores moleculares
653 $aMolecular markers
653 $aPastagens
653 $aPasture
653 $aVariabilidade genética
700 1 $aMACHADO NETO, N. B.
700 1 $aGUABERTO, L. M.
700 1 $aVANDERLEI, T. M.
773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 10, n. 1, p. 55-64, Mar. 2010.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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