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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
16/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GUIMARAES, A. S.; DORNELES, E. M. S.; ANDRADE, G. I.; LAGE, A. P.; MIYOSHI, A.; AZEVEDO, V.; GOUVEIA, A. M. G.; HEINEMANN, M. B. |
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Afiliação: |
ALESSANDRO DE SA GUIMARAES, CNPGL; ELAINE MARIA SELES DORNELES, UFMG; GIOVANNA IVO ANDRADE, UFMG; ANDREY PEREIRA LAGE, UFMG; GEPOC; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG; GEPOC; AURORA MARIA GUIMARÃES GOUVEIA, UFMG; GEPOC; MARCOS BRYAN HEINEMANN, UFMG; GEPOC. |
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Título: |
Molecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 153, n. 3/4, p. 299-306, 2011. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2011.06.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates. MenosCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10... Mostrar Tudo |
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Palavras-Chave: |
ERIC-PCR; RAPD. |
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Thesagro: |
Corynebacterium Pseudotuberculosis. |
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Thesaurus Nal: |
caseous lymphadenitis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02507naa a2200265 a 4500
001 1910089
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2011.06.002$2DOI
100 1 $aGUIMARAES, A. S.
245 $aMolecular characterization of Corynebacterium pseudotuberculosis isolates using ERIC-PCR.$h[electronic resource]
260 $c2011
520 $aCaseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
650 $acaseous lymphadenitis
650 $aCorynebacterium Pseudotuberculosis
653 $aERIC-PCR
653 $aRAPD
700 1 $aDORNELES, E. M. S.
700 1 $aANDRADE, G. I.
700 1 $aLAGE, A. P.
700 1 $aMIYOSHI, A.
700 1 $aAZEVEDO, V.
700 1 $aGOUVEIA, A. M. G.
700 1 $aHEINEMANN, M. B.
773 $tVeterinary Microbiology$gv. 153, n. 3/4, p. 299-306, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 8 | |
| 1. |  | SOUZA, V. B. de; CARVALHO, A. J. de; SILVA, K. J. D. e; ROCHA, M. de M.; LACERDA, M. L.; PEREIRA FILHO, I. A. Agronomic performance of cowpea elite lines in the States of Minas Gerais and Mato Grosso, Brazil. Revista Caatinga, Mossoró, v. 31, n. 1, p. 90-98, jan./mar. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 2. | | SOUZA, V. B. de; CARVALHO, A. J. de; SOUZA, A. A. de; LACERDA, M. L.; ROCHA, M. de M.; SILVA, K. J. D. e; PEREIRA FILHO, I. A. Avaliação do porte de plantas de genótipos de feijão-caupi de porte prostrado e semiprostrado em ambientes de Minas Gerais e Mato Grosso. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercados: resumos. Brasília, DF: Embrapa, 2016. p. 51.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 3. | | SOUZA, V. B. de; CARVALHO, A. J. de; SILVA, K. J. D. e; ROCHA, M. de M.; PEREIRA FILHO, I. A.; LACERDA, M. L.; SOUZA, A. A. de. Índice de grãos e comprimento de vagens de linhagens-elite de feijão-caupi de porte ereto e semiereto em ambientes de Minas Gerais e Mato Grosso. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. p. 83. Na publicação: Kaesel Jackson Damasceno-Silva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 4. | | SOUZA, V. B. de; CARVALHO, A. J. de; SILVA, K. J. D. e; ROCHA, M. de M.; PEREIRA FILHO, I. A.; MEDEIROS, J. C.; LACERDA, M. L. Grau de acamamento de genótipos de feijão-caupi de porte prostrado em diferentes ambientes de Minas Gerais e Mato Grosso. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercados: resumos. Brasília, DF: Embrapa, 2016. p. 79. Na publicação: Kaesel Jackson Damasceno-Silva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte; Embrapa Milho e Sorgo. |
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| 5. | | LACERDA, M. L.; ASPIAZU, I.; CARVALHO, A. J. de; SILVA, A. F. da; FERREIRA, E. A.; SOUZA, A. A. de; CAMPOS, M. L. de; BRITO, C. F. B. Periods of weed interference in cowpea crop in the semi-arid of Minas Gerais, Brazil. Revista Brasileira de Ciências Agrárias, v. 15, n. 2, e6749, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | BARBOSA, F. R.; PERIN, A.; DUARTE, A. R.; QUINTELA, E. D.; HIROSE, E.; GIELFI, F. G.; JESUS, F. G. de; MIRANDA, J. E.; CRUZ, I.; VALE, L. S. R.; LACERDA, M. C.; BELLIZZI, N. C.; BARRETTO, V. C. de M. Capacitação, comunicação e treinamento de multiplicadores em Manejado Integrado de Pragas (MIP). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 26.; CONGRESSO LATINO-AMERICANO DE ENTOMOLOGIA, 9., 2016, Maceió. Anais... Brasília, DF : Embrapa, 2016. p. 578.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Milho e Sorgo. |
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| 7. | | BRITO, O. G.; CARNEIRO, J. E. de S.; MOREIRA, J. A. A.; MARTINS, M.; MELO, L. C.; FARIA, L. C.; PEREIRA, H. S.; OLIVEIRA, M. B. de; MEDEIROS, J. C.; SOUZA, T. L. P. O.; BATISTA, P. S. C.; LACERDA, M. L. Productivity, adaptability and stability of production of special grain common bean lines in different environments of Minas Gerais, Brazil. Annual Report of the Bean Improvement Cooperative, Prosser, v. 59, p. 251-252, Apr. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Milho e Sorgo. |
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| 8. | | DEMATTÊ, J. A. M.; DOTTO, A. C.; PAIVA, A. F. S.; SATO, M. V.; DALMOLIN, R. S. D.; ARAÚJO, M. do S. B. de; SILVA, E. B. da; NANNI, M. R.; CATEN, A. ten; NORONHA, N. C.; LACERDA, M. P. C.; ARAUJO FILHO, J. C. de; RIZZO, R.; BELLINASO, H.; FRANCELINO, M. R.; SCHAEFER, C. E. G. R.; VICENTE, L. E.; SANTOS, U. J. dos; SAMPAIO, E. V. de S. B.; MENEZES, R. S. C.; SOUZA, J. J. L. L. de; ABRAHÃO, W. A. P.; COELHO, R. M.; GREGO, C. R.; LANI, J. L.; FERNANDES, A. R.; GONÇALVES, D. A. M.; SILVA, S. H. G.; MENEZES, M. D. de; CURI, N.; COUTO, E. G.; ANJOS, L. H. C. dos; CEDDIA, M. B.; PINHEIRO, E. F. M.; GRUNWALD, S.; VASQUES, G. de M.; MARQUES JÚNIOR, J.; SILVA, A. J. da; BARRETO, M. C. de V.; NÓBREGA, G. N.; SILVA, M. Z. da; SOUZA, S. F. de; VALLADARES, G. S.; VIANA, J. H. M.; TERRA, F. da S.; HORÁK-TERRA, I.; FIORIO, P. R.; SILVA, R. C. da; FRADE JÚNIOR, E. F.; LIMA, R. H. C.; FILIPPINI ALBA, J. M.; SOUZA JUNIOR, V. S. de; BREFIN, M. de L. M. S.; RUIVO, M. de L. P.; FERREIRA, T. O.; BRAIT, M. A.; CAETANO, N. R.; BRINGHENTI, I.; MENDES, W. de S.; SAFANELLI, J. L.; GUIMARÃES, C. C. B.; POPPIEL, R. R.; SOUZA, A. B. e; QUESADA, C. A.; COUTO, H. T. Z. do. The Brazilian Soil Spectral Library (BSSL): a general view, application and challenges. Geoderma, v. 354, 113793, 2019. Na publicação: Gustavo M. Vasques.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Clima Temperado; Embrapa Maranhão; Embrapa Meio Ambiente; Embrapa Milho e Sorgo; Embrapa Solos. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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