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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/01/2015 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, P. A. S; ROSSE, I. C.; DEMIRANDA, M.; MACHADO, M. A.; VERNEQUE, R. da S.; PEIXOTO, M. G. C. D.; CARVALHO, M. R. S. |
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Afiliação: |
P. A. S. FONSECA, UFMG; I. C. ROSSE, UFMG; M. DEMIRANDA, UFMG; MARCO ANTONIO MACHADO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; M. R. S. CARVALHO, UFMG. |
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Título: |
A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 12, n. 4, p. 6521-6526, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetra-primer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphisms. |
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Palavras-Chave: |
CSN3; K-Casein; K-Casein polymorphism; Tetra-primer ARMS-PCR. |
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Thesaurus Nal: |
casein. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02080naa a2200253 a 4500
001 2006643
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aFONSECA, P. A. S
245 $aA new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms.$h[electronic resource]
260 $c2014
520 $aKappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduces the loss of milk solids, enhancing cheese yield. This polymorphism induces a double amino acid substitution (Thr136Ile and Ala148Asp). The polymorphism is normally detected by PCR-RFLP, which is a laborious method. An interesting methodological alternative is the tetra-primer amplification refractory mutation system PCR (tetra-primer ARMS-PCR). A tetra-primer ARMS-PCR for the detection of this κ-casein polymorphism has been described. However, specificity was not achieved, probably due to problems with primer design. We developed a new tetra-primer ARMS-PCR for the detection of the κ-casein polymorphism. This new method was validated in a double-blind test, by comparison with the results obtained for 50 Guzerá bulls formerly genotyped by PCR-RFLP. This new method achieved 100% sensitivity and specificity. We conclude that this method is a useful, cost-efficient alternative for the detection of functional κ-casein polymorphisms.
650 $acasein
653 $aCSN3
653 $aK-Casein
653 $aK-Casein polymorphism
653 $aTetra-primer ARMS-PCR
700 1 $aROSSE, I. C.
700 1 $aDEMIRANDA, M.
700 1 $aMACHADO, M. A.
700 1 $aVERNEQUE, R. da S.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aCARVALHO, M. R. S.
773 $tGenetics and Molecular Research$gv. 12, n. 4, p. 6521-6526, 2014.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 10 | |
| 3. |  | VEREDIANO, T. A.; AGARWAL, N.; MARTINO, H. S. D.; KOLBA, N.; GRANCIERI, M.; PAES, M. C. D.; TAKO, E. Effect of black corn anthocyanin-rich extract (Zea mays L.) on cecal microbial populations in vivo (Gallus gallus). Nutrients, v. 14, n. 21, 4679, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 4. | | FORTINI, T. V. L.; VEREDIANO, T. A.; SANT'ANA, C. T.; GRANCIERI, M.; SILVA, B. P. da; PAES, M. C. D.; MARTINO, H. Black corn (Zea mays L.) flour consumption improved the gut dysbiosis promoted by a high-fat diet on mice. In: INTERNATIONAL CONGRESS ON BIOACTIVE COMPOUNDS, 2.; INTERNATIONAL WORKSHOP ON BIOACTIVE COMPOUNDS, 3., 2022, Campinas. Personal nutrition, ageing and food science: proceedings. Campinas: Unicamp, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | VEREDIANO, T. A.; SANT'ANA, C. T.; GRANCIERI, M.; SÃO JOSÉ, V. P. B. de; TOLEDO, R. C. L.; PAES, M. C. D.; MARTINO, H. S. D. Black corn (Zea mays L.) whole flour improved the antioxidant capacity and prevented adipogenesis in mice fed a high-fat diet. Food & Function, v. 13, p. 5590-5601, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 6. | | MARTINEZ, O. D. M.; THEODORO, J. M. V.; GRANCIERI, M.; TOLEDO, R. C. L.; QUEIROZ, V. A. V.; BARROS, F. A. R. de; MARTINO, H. S. D. Dry heated whole sorghum flour (BRS 305) with high tannin and resistant starch improves glucose metabolism, modulates adiposity, and reduces liver steatosis and lipogenesis in Wistar rats fed with a high-fat high-fructose diet. Journal of Cereal Science, v. 99, 103201, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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| 7. | | LUCIO, H. G.; LOPES, R. de K. S. O.; GOMES, M. J. C.; GRANCIERI, M.; QUEIROZ, V. A. V.; SILVA, B.; MARTINO, H. S. D. Refeição simbiótica contendo sorgo extrusado associado a probiótico (Bifidobacterium longum) melhora saúde intestinal sem alterar a microbiota intestinal de pacientes com doença renal crônica. In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | MARTINO, H.; MARTINEZ, O. D. M.; GRANCIERI, M.; GOMES, M. J. C.; SILVA, B. P. da; SÃO JOSÉ, V.; TOLEDO, R. C. L.; QUEIROZ, V. A. V. Sorghum flour BRS 305 hybrid has the potential to modulate the intestinal microbiota of rats fed with a high-fat high-fructose diet. In: INTERNATIONAL CONGRESS ON BIOACTIVE COMPOUNDS, 2.; INTERNATIONAL WORKSHOP ON BIOACTIVE COMPOUNDS, 3., 2022, Campinas. Personal nutrition, ageing and food science: proceedings. Campinas: Unicamp, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | MARTINEZ, O. D. M.; THEODORO, J. M. V.; GRANCIERI, M.; TOLEDO, R. C. L.; BARROS, F. A. R. de; TAKO, E.; QUEIROZ, V. A. V.; MARTINO, H. S. D. Dry heated sorghum BRS 305 hybrid flour as a source of resistant starch and tannins improves inflammation and oxidative stress in Wistar rats fed with a high-fat high-fructose diet. Food & Function, v. 12, p. 8738-8746, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 10. | | LÚCIO, H. G.; GRANCIERI, M.; MARTINEZ, O. D. M.; TOLEDO, R. C. L.; MENEZES, C. B. de; COSTA, N. M. B.; QUEIROZ, V. A. V.; SILVA, B. P. da; MARTINO, H. S. D. Dry heat whole Sorghum BRS 305 flour modulate satiety and improves antioxidant response in brain of Wistar rats fed with high-fat high-fructose diet. Food Research International, v. 173, 113252, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| Registros recuperados : 10 | |
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