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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/01/2020 |
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Data da última atualização: |
23/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. |
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Afiliação: |
VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINA CERQUEIRA SARMENTO OLIVARES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUANA RANGEL CÔRTES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. |
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DOI: |
10.22456/1679-9216.98317 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. MenosBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, ... Mostrar Tudo |
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Palavras-Chave: |
Billygoats; CTC. |
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Thesagro: |
Bode; Bovino; Caprino; Carneiro; Espermatozóide; Heparina; Ovino; Reprodução Animal; Sêmen. |
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Thesaurus Nal: |
Animal reproduction; Assisted reproductive technologies; Bulls; Cattle; Goats; Heparin; Rams; Sheep; Sperm capacitation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209450/1/cnpc-2019-Mini-Percoll.pdf
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Marc: |
LEADER 04821naa a2200469 a 4500
001 2119218
005 2020-01-23
008 2019 bl uuuu u00u1 u #d
024 7 $a10.22456/1679-9216.98317$2DOI
100 1 $aALFRADIQUE, V. A. P.
245 $aMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.$h[electronic resource]
260 $c2019
520 $aBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique.
650 $aAnimal reproduction
650 $aAssisted reproductive technologies
650 $aBulls
650 $aCattle
650 $aGoats
650 $aHeparin
650 $aRams
650 $aSheep
650 $aSperm capacitation
650 $aBode
650 $aBovino
650 $aCaprino
650 $aCarneiro
650 $aEspermatozóide
650 $aHeparina
650 $aOvino
650 $aReprodução Animal
650 $aSêmen
653 $aBillygoats
653 $aCTC
700 1 $aOLIVARES, C. C. S.
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aBALARO, M. F. A.
700 1 $aSARAIVA, H. F. R. de A.
700 1 $aCÔRTES, L. R.
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1707, p. 1-8, 2019.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 275 | |
| 261. |  | OLIVEIRA-PAIVA, C. A.; COTA, L. V.; MARRIEL, I. E.; GOMES, E. A.; SOUSA, S. M. de; LANA, U. G. de P.; SANTOS, F. C. dos; PINTO JUNIOR, A. S.; ALVES, V. M. C. Viabilidade técnica e econômica do Biomaphos® (Bacillus subtilis CNPMS B2084 e Bacillus megaterium CNPMS B119) nas culturas de milho e soja. Sete Lagoas: Embrapa Milho e Sorgo, 2020. 20 p. (Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 210). ODS 12.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 262. | | ABREU, C. S.; GOMES, E. A.; OLIVEIRA, J. C. M. D.; OLIVEIRA-PAIVA, C. A.; MORAIS, D. K.; SILVA, U. C.; SANTOS, V. L.; GOES NETO, A.; FIGUEIREDO, J. E. F.; STOIANOFF, M. A. R. Study on the effects of mineral phosphate fertilizers on soil bacterial communities of tropical agroecosystems. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 29.; ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 15., 2017, Foz do Iguaçu. Resumos... São Paulo: Sociedade Brasileira de Microbiologia, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 263. | | ABREU, C. S.; BATISTA, F. C.; OLIVEIRA, J. C. M. D.; BONIEK, D.; OLIVEIRA-PAIVA, C. A.; GOMES, E. A.; LANA, U. G. P.; SANTOS, V. L.; STOIANOFF, M. A. R. Seleção de bactérias rizosféricas de milho eficientes na biossolubilização de fosfato natural de Araxá. In: SIMPÓSIO DE MICROBIOLOGIA DA UFMG, 4., 2017, Belo Horizonte. Metabolismo microbiano: saúde, ambiente e biotecnologia: resumos. Belo Horizonte: UFMG, 2017. p. 190.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 264. | | ABREU, C. S. de; OLIVEIRA-PAIVA, C. A.; FIGUEIREDO, J. E. F.; TAVARES, A. N. G.; GOMES, E. A.; SANTOS, V. L. dos; MARRIEL, I. E.; STOIANOFF, M. A. de R. Seleção e caracterização de bacterias endofiticas de milho com potencial para biossolubilização de fosfato. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. p. 1004. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 265. | | OLIVEIRA-PAIVA, C. A.; MARRIEL, I. E.; ALVES, V. M. C.; GOMES, E. A.; MONTEIRO, G. G.; RAPOSIERAS, R.; CARNEIRO, N. P.; SCHAFFERT, R. E.; SCOTTI, M. R.; SÁ, N. M. H. de. Seleção e composição filogenética de comunidades microbianas biomineralizadoras e biossolubilizadoras de fósforo em milho sob plantio direto no cerrado. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 23., 2005, Santos, SP. Resumos. Santos: Sociedade Brasileira de Microbiologia, 2005. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 266. | | GOMES, E. A.; OLIVEIRA-PAIVA, C. A.; CARNEIRO, N. P.; MARRIEL, I. E.; LANA, U. G. P.; RAPOSEIRAS, R.; SA, N. M. H.; MUZZI, M. R. S.; SCHAFFERT, R. E.; GUIMARAES, C. T.; ALVES, V. M. C. Rhizosphere and root mycorrhizal diversity of maize genotypes contrasting for phosphorus efficiency. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 267. | | SOUSA, S. M. de; OLIVEIRA-PAIVA, C. A.; ANDRADE, D. L.; CARVALHO, C. G. de; RIBEIRO, V. P.; PASTINA, M. M.; MARRIEL, I. E.; LANA, U. G. de P.; GOMES, E. A. Tropical Bacillus strains inoculation enhances maize root surface area, dry weight, nutrient uptake and grain yield. Journal of Plant Growth Regulation, v. 40, p. 867-877, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 268. | | VELLOSO, C. C. V.; RIBEIRO, V. P.; CARVALHO, C. G. de; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; MARRIEL, I. E.; SOUSA, S. M. de; GOMES, E. A. Tropical endophytic Bacillus species enhance plant growth and nutrient uptake in cereals. In: MAHESHWARI, D. K.; DHEEMAN, D. (ed.). Endophytes: mineral nutrient management. Cham: Springer, 2021. v. 3, p. 157-180. (Sustainable Development and Biodiversity, 26).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 269. | | NODA, R. W.; GUIMARAES, C. T.; GOMES, E. A.; CARNEIRO, N. P.; LANA, U. G. de P.; MAGALHAES, J. V. de; COSTA, M. M. do C.; SILVA, F. R. D.; BRAMMER, S. P.; LÂNGARO, N. C.; CARVALHO, L. J. C. B.; BEVITORI, R.; PURCINO, A. A. C. Aluminum-induced genes in grass species. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 6., 2010, Ouro Preto. Abstracts... [S.l.: s.n.], 2010. p. 185. AB3C X-meeting 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 270. | | FIGUEIREDO, J. E. F.; TEIXEIRA, M. A.; LIMA, G. V. C.; NEVES, N. A.; MARRA, L. M.; SILVA, C. H. C.; LOPES, S. C.; LANA, U. G. de P.; GOMES, E. A.; GRAZIOTTI, P. H.; FLATSCHART, A. V. F.; BRESSAN, W. Identificação molecular da comunidade bacteriana associada à rizosfera de plantas sempre-viva (Syngonanthus elegans var. elegans) endêmica de campos rupestres brasileiros. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 77-78.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 271. | | OLIVEIRA-PAIVA, C. A.; MARRIEL, I. E.; GOMES, E. A.; COTA, L. V.; SANTOS, F. C. dos; SOUSA, S. M. de; LANA, U. G. de P.; OLIVEIRA, M. C.; MATTOS, B. B.; ALVES, V. M. C.; RIBEIRO, V. P.; VASCO JUNIOR, R. Recomendação agronômica de cepas de Bacillus subtilis (CNPMS B2084) e Bacillus megaterium (CNPMS B119) na cultura do milho. Sete Lagoas: Embrapa Milho e Sorgo, 2020. 18 p. (Embrapa Milho e Sorgo. Circular Técnica, 260). ODS 12.| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Solos. |
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| 272. | | GUIMARÃES, S. da S. C.; TAVARES, D. G.; MONTEIRO, M. C. P.; PEDROSO, M. P.; NUNES, C. A.; MOURÃO, B.; CARVALHO, I. S. e; BARDAJÍ, D. K. R.; CAMARGO, I. L. B. C.; LANA, U. G. de P.; GOMES, E. A.; QUEIROZ, M. V. de; PEREIRA, O. L.; CARDOSO, P. G. Polyphasic characterization and antimicrobial properties of Induratia species isolated from Cofea arabica in Brazil. Mycological Progress, v. 20, p. 1457-1477, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 273. | | CARNEIRO, A. A.; GOMES, E. A.; SOUZA, E. T. da S.; COELHO, G. T. da C. P.; MARRIEL, I. E.; PEREIRA, M. de F.; VASCONCELOS, M. J. V. de; OLIVEIRA, M. T. S. de; CARNEIRO, N. P.; SILVA, P. G. da. Fungos mineralizadores de fitato isolados da risosfera de milho. Sete Lagoas: Embrapa Milho e Sorgo, 2008. 10 p. (Embrapa Milho e Sorgo. Circular técnica, 109).| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 274. | | PARENTONI, S. N.; ALVES, V. M. C.; MARRIEL, I. E.; GOMES, E. A.; OLIVEIRA, C.; GUIMARAES, C. T.; GUIMARAES, P. E. O.; PACHECO, C. A. P.; MEIRELLES, W. F.; GUIMARAES, L. J. M.; LANA, U. G. de P.; SILVA, A. R. da; VASCONCELOS, M. J. V. de; MAGALHAES, J. V. de; COELHO, A. M.; SCHAFFERT, R. E. Plantas eficientes e bio-rochas: mudando paradigmas na agricultura tropical. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 1., 2008, Brasília, DF. Resumos... Brasília, DF: Embrapa, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 275. | | OLIVEIRA-PAIVA, C. A.; COTA, L. V.; MARRIEL, I. E.; ALVES, V. M. C.; GOMES, E. A.; SOUSA, S. M. de; SANTOS, F. C. dos; SOUZA, F. F. de; LANDAU, E. C.; PINTO JUNIOR, A. S.; LANA, U. G. de P. Validação da recomendação para o uso do inoculante BiomaPhos® (Bacillus subtilis CNPMS B2084 e Bacillus megaterium CNPMS B119) na cultura de soja. Sete Lagoas: Embrapa Milho e Sorgo, 2021. 18 p. (Embrapa Milho e Sorgo. Circular Técnica, 279). ODS 12.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 275 | |
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