| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
07/05/2013 |
|
Data da última atualização: |
17/01/2024 |
|
Autoria: |
MARTINS, F. S.; SARAIVA, M. V. A.; CELESTINO, J. J. H.; BRUNO, J. B.; ALMEIDA, A. P.; CUNHA, R. M. S.; SILVA, J. R. V.; CAMPELO, C. C.; LUCCI, C. M.; MATOS, M. H. T.; FIGUEIREDO, J. R. |
|
Título: |
Expression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
Animal Reproduction, v. 7, n. 4, p. 349-361, Oct./Dec. 2010. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles. MenosAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed ... Mostrar Tudo |
|
Palavras-Chave: |
Folículo pré-antral; IGF-I. |
|
Thesagro: |
Cabra; Caprino; Endocrinologia; Fator de crescimento; Hormônio; Insulina; Ovário; Reprodução animal. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02813naa a2200361 a 4500
001 1957241
005 2024-01-17
008 2010 bl uuuu u00u1 u #d
100 1 $aMARTINS, F. S.
245 $aExpression of protein and mRNA encoding Insulin Growth Factor-I (IGF-I) in goat ovarian follicles and the influence of IGF-I on in vitro development and survival of caprine preantral follicles.$h[electronic resource]
260 $c2010
520 $aAbstract: The aim of this study was to investigate the protein and mRNA expression levels of IGF-I and the effects of IGF-I on preantral follicle survival and development, using an in vitro goat ovarian cortical culture system. The ovaries were used for immunohistochemical localization of IGF-I protein or used to demonstrate mRNA expression of IGF-I. For the latter goal, preantral and antral follicles, cumulus-oocyte complex, mural granulosa and theca cells were collected to study mRNA expression. For in vitro studies, ovarian cortex were cultured for 1 and 7 days with MEM supplemented IGF-I (0, 1, 10, 50, 100 or 200 ng/ml). Immunohistochemical results showed strong reactions for IGF-I in oocytes and granulosa cells of all follicular stages, except in granulosa cells of primordial and primary follicles. mRNA expression analysis demonstrated a discrete increase in the production of IGF-I during the transition from primordial to the primary and secondary follicle stages. After 7 days of culture, addition of 50 ng/ml of IGF-I to the medium showed the greatest percentage of normal follicles when compared with other concentrations. Furthermore, the highest percentage of primary follicles was observed after 7 days of culture in MEM+plus 10 and 50 ng/ml of IGF-I. Culture of ovarian tissue for 7 days in MEM+plus 50 ng/ml of IGF-I promoted the greatest increase (P < 0.05) in follicular diameter of all of the concentrations tested. In conclusion, IGF-I protein and mRNA were expressed in all follicular categories of goat. Furthermore, IGF-I maintained preantral follicle survival, promoted primordial follicle activation and stimulated the transition from intermediate to primary follicles.
650 $aCabra
650 $aCaprino
650 $aEndocrinologia
650 $aFator de crescimento
650 $aHormônio
650 $aInsulina
650 $aOvário
650 $aReprodução animal
653 $aFolículo pré-antral
653 $aIGF-I
700 1 $aSARAIVA, M. V. A.
700 1 $aCELESTINO, J. J. H.
700 1 $aBRUNO, J. B.
700 1 $aALMEIDA, A. P.
700 1 $aCUNHA, R. M. S.
700 1 $aSILVA, J. R. V.
700 1 $aCAMPELO, C. C.
700 1 $aLUCCI, C. M.
700 1 $aMATOS, M. H. T.
700 1 $aFIGUEIREDO, J. R.
773 $tAnimal Reproduction$gv. 7, n. 4, p. 349-361, Oct./Dec. 2010.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | KUBO, K. S.; STUART, R. M.; BASTIANEL, M.; COSTA, F. N.; FREITAS-ASTÚA, J.; MACHADO, M. A. Response of Murcott tangor and Pera sweet orange to Citrus leprosis virus C (CiLV-C) and Brevipalpus phoenicis mites analyzed by 2DE. In:JOINT ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS AND THE CANADIAN SOCIETY OF PLANT PHYSIOLOGISTS, 2009, Honolulu, Hawaii. Plant Biology Meeting: final program. [S.n.], 2009. P48071| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 2. | | KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
