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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/12/2016 |
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Data da última atualização: |
07/02/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; LEDO, C. A. da S. |
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Afiliação: |
Á. M. VIDAL, UFRB; L. J. VIEIRA, UFRB; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers BRS Formosa: variedade de mandioca recomendada para uso industrial nas microrregiões de Valença, Jequié e Santo Antônio de Jesus (BA). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.14, N.3,p. 7759-7770, 2015. |
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Idioma: |
Português |
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Conteúdo: |
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic omplement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. MenosMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic omplement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava pl... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Cassava. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02339naa a2200193 a 4500
001 2059227
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008 2015 bl uuuu u00u1 u #d
100 1 $aVIDAL, Á. M.
245 $aGenetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers BRS Formosa$bvariedade de mandioca recomendada para uso industrial nas microrregiões de Valença, Jequié e Santo Antônio de Jesus (BA).$h[electronic resource]
260 $c2015
520 $aMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic omplement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.
650 $aCassava
650 $aMandioca
700 1 $aVIEIRA, L. J.
700 1 $aFERREIRA, C. F.
700 1 $aSOUZA, F. V. D.
700 1 $aLEDO, C. A. da S.
773 $tGenetics and Molecular Research$gv.14, N.3,p. 7759-7770, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 6 | |
| 1. |  | SILVA NETO, A. M. da; BRAVO, I. S. J.; SANTOS, D.; DAMASCENO, I.; SANTOS, D.; VIRGÍNIO, J.; PARANHOS, B. A. J.; NASCIMENTO, A. S. do. Efeito da exposição permanente a luminosidade versus foto período sobre a produção e qualidade dos ovos em criação massal de Ceratitis capitata. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. pdf P357| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | SILVA NETO, A. M. da; NASCIMENTO, A. S. do; BRAVO, I. S. J.; DIAS, V. S.; BENEVIDES, C. M. de J.; SILVA, M. V. L.; BENEVIDES, L.; OLIVEIRA, G. B. de. Fontes protéicas oriundas de produtos comerciais nacionais como potenciais substitutos do hidrolisado importado para a criação massal de Ceratitis capitata. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | BENEVIDES, L. de J.; SILVA NETO, A. M. da; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; JOACHIM-BRAVO, I. S.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Low-cost artificial diets for the Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae). Magistra, Cruz das Almas-BA, v. 24, número especial, p. 01-07, dez. 2012. Semana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | SILVA NETO, A. M. da S.; BENEVIDES, L. de J.; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; JOACHIM-BRAVO, I. S.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Resíduos agroindustriais na alimentação da mosca do Mediterrâneo (Diptera: Tephritidae). In:CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. pdf P444| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | BENEVIDES, L. de J.; SILVA NETO, A. M. da; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; BRAVO, I. S. J.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Subprodutos agroindustriais na elaboração de dietas para moscas-das-frutas (Ceratitis capitata). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | SILVA NETO, A. M. da; SANTOS, T. R. de O.; DIAS, V. S.; BRAVO, I. S. J.; BENEVIDES, L. de J.; BENEVIDES, C. M. de J.; SILVA, M. V. L.; SANTOS, D. C. C. dos; VIRGÍNIO, J.; OLIVEIRA, G. B.; WALDER, J. M. M.; PARANHOS, B. A. J.; NASCIMENTO, A. S. do. Mass-rearing of Mediterranean fruit fly using low-cost yeast products produced in Brazil. Scientia Agricola, v. 69, n. 6, p. 364-369, nov./dez. 2012| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Semiárido. |
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| Registros recuperados : 6 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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