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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MARTINS, P. K.; MAFRA, V. S.; KISHI, L. T.; FREITAS-ASTUA, J. |
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Afiliação: |
Polyanna Kelly Martins, APTA; Valéria S. Mafra, APTA; Luciano T. Kishi, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Phylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 35130 |
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Conteúdo: |
Plants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation. MenosPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise... Mostrar Tudo |
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Palavras-Chave: |
CitEST; SAR; W-box. |
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Thesaurus Nal: |
Citrus; transcription factors. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03255nam a2200217 a 4500
001 1886952
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aMARTINS, P. K.
245 $aPhylogenetic and comparative gene expression analysis of citrus WRKY transcription factor family.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 35130
520 $aPlants have a variety of active defense mechanisms to protect themselves from pathogen infection. Plant defense response results from the transcriptional activation of a large number of genes upon pathogen infection or treatment with pathogen elicitors. Among them, WRKY proteins are considered important transcriptional regulators in defense signaling, including the activation of SAR (systemic acquired resistance). Several genes regulating plant defense, such as NPR1 and PR (Pathogenesis-related proteins), have W-box elements in their promoters that are specifically recognized by WRKY proteins also required for induction or expression. The defining feature of WRKY transcription factors is their DNA binding domain. The WRKY domain is about 60 residues in length that contains the WRKY signature and also an atypical zinc-finger structure at the C-terminus (Cx4?5Cx22?23HxH or Cx7Cx23HxC). The aim of this work was to identify all potential WRKY transcription factors in citrus, retrieved from the CitEST (Citrus ESTs) database and to construct a phylogenetic tree with orthologs from Arabidopsis thaliana and Oryza sativa. To find ESTs coding for WRKY proteins in citrus we performed a tBLASTn search on the CitEST database. The WRKY domain sequences of seven Arabidopsis WRKY family members each representing one of the WRKY subgroups were used as query sequences. Phylogenetic tree were constructed by the neighbor-joining (NJ) method in MEGA 4. NJ analysis was performed with the Pairwise Deletion option and the Poisson correction. For statistical reliability, bootstrap analysis was conducted with 1,000 replicates to assess statistical support for each node. The primary search resulted in 71 non-redundant hits, of which 9 were removed as they did not contain the conserved WRKY domain signature. The remaining 62 sequences were used to construct the phylogenetic tree; this analysis identified 19 unigenes for the group 1; 6 unigenes for the subgroup 2a, 3 unigenes for the subgroup 2b, 12 unigenes for the subgroup 2c, 13 unigenes for the group 2d, 9 unigenes for subgroup 2e and 7 unigenes for the group 3. To complete the phylogenetic analysis, WRKY sequences from the draft sequencing of the genomes of Citrus clementina and C. sinensis will be included. We are particularly interested in WRKY proteins involved in biotic stress, so the next step will be to evaluate the transcriptional profile of some WRKY genes under different biotic stress conditions. This study will be crucial to select candidates for citrus genetic transformation.
650 $aCitrus
650 $atranscription factors
653 $aCitEST
653 $aSAR
653 $aW-box
700 1 $aMAFRA, V. S.
700 1 $aKISHI, L. T.
700 1 $aFREITAS-ASTUA, J.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 7 | |
| 1. |  | SILVA NETO, A. M. da; BRAVO, I. S. J.; SANTOS, D.; DAMASCENO, I.; SANTOS, D.; VIRGÍNIO, J.; PARANHOS, B. A. J.; NASCIMENTO, A. S. do. Efeito da exposição permanente a luminosidade versus foto período sobre a produção e qualidade dos ovos em criação massal de Ceratitis capitata. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. pdf P357| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | NASCIMENTO, A. S. do; JOACHIM-BRAVO, I. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; OLIVEIRA, G. B. de; BENEVIDES, L. de J.; SILVA NETO, A. M.; DIAS, V. S. Aprimorando a técnica do inseto estéril para fruticultura do Semiárido: avaliação de dietas de baixo custo para criação massal da mosca do mediterrâneo, ceratitis capitata. In: FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DA BAHIA. Semiárido: Edital Temático de Apoio a Pesquisas voltadas à resolução de problemas do Semiárido baiano 2007. Salvador, 2012, p. 135-140.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | SILVA NETO, A. M. da; NASCIMENTO, A. S. do; BRAVO, I. S. J.; DIAS, V. S.; BENEVIDES, C. M. de J.; SILVA, M. V. L.; BENEVIDES, L.; OLIVEIRA, G. B. de. Fontes protéicas oriundas de produtos comerciais nacionais como potenciais substitutos do hidrolisado importado para a criação massal de Ceratitis capitata. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 4. | | BENEVIDES, L. de J.; SILVA NETO, A. M. da; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; JOACHIM-BRAVO, I. S.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Low-cost artificial diets for the Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae). Magistra, Cruz das Almas-BA, v. 24, número especial, p. 01-07, dez. 2012. Semana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | SILVA NETO, A. M. da S.; BENEVIDES, L. de J.; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; JOACHIM-BRAVO, I. S.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Resíduos agroindustriais na alimentação da mosca do Mediterrâneo (Diptera: Tephritidae). In:CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. pdf P444| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | BENEVIDES, L. de J.; SILVA NETO, A. M. da; DIAS, V. S.; BENEVIDES, C. M. de J.; LOPES, M. V.; BRAVO, I. S. J.; OLIVEIRA, G. B. de; NASCIMENTO, A. S. do. Subprodutos agroindustriais na elaboração de dietas para moscas-das-frutas (Ceratitis capitata). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 3., 2009, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2009. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | SILVA NETO, A. M. da; SANTOS, T. R. de O.; DIAS, V. S.; BRAVO, I. S. J.; BENEVIDES, L. de J.; BENEVIDES, C. M. de J.; SILVA, M. V. L.; SANTOS, D. C. C. dos; VIRGÍNIO, J.; OLIVEIRA, G. B.; WALDER, J. M. M.; PARANHOS, B. A. J.; NASCIMENTO, A. S. do. Mass-rearing of Mediterranean fruit fly using low-cost yeast products produced in Brazil. Scientia Agricola, v. 69, n. 6, p. 364-369, nov./dez. 2012| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Semiárido. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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