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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
21/01/1997 |
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Data da última atualização: |
13/09/2013 |
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Autoria: |
BOKULICH, N.; HWANG, C. F.; LIU, S.; MILLS, K. L. B.; MILLS, D. A. |
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Título: |
Profiling the yeast communities of wine fermentations using terminal restriction fragment length polymorphism analysis. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
American Journal of Enology and Viticulture, Reedley, v. 63, n. 2, p. 185-194, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Terminal restriction fragment length polymorphism (TRFLP), used most often to describe bacterial communities, presents a high-throughput, low-cost solution for analyzing mixed yeast communities in wine and other fermentations. In this study, a TRFLP approach was developed for the identification and discrimination of yeasts and used to construct a TRFLP database comprising 121 strains of yeast (representing 24 genera and 72 species) associated with wine and food fermentations. This database exhibits sensitive discrimination among species and robust intraspecific conservation of TRFLP profiles, enabling reliable characterization of mixed yeast communities. The yeast ecology of sweet, botrytized wine fermentations from two separate vintages was analyzed using this database, demonstrating the utility of this method for fast-paced, qualitative detection and identification of differences in yeast community structures over time in a complex, diverse fermentation system. |
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Palavras-Chave: |
Levedura; TRFLP. |
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Thesagro: |
Fermentação; Uva; Vinho; Vitis Vinifera. |
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Thesaurus Nal: |
Grapes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01713naa a2200253 a 4500
001 1127961
005 2013-09-13
008 2012 bl uuuu u00u1 u #d
100 1 $aBOKULICH, N.
245 $aProfiling the yeast communities of wine fermentations using terminal restriction fragment length polymorphism analysis.$h[electronic resource]
260 $c2012
520 $aTerminal restriction fragment length polymorphism (TRFLP), used most often to describe bacterial communities, presents a high-throughput, low-cost solution for analyzing mixed yeast communities in wine and other fermentations. In this study, a TRFLP approach was developed for the identification and discrimination of yeasts and used to construct a TRFLP database comprising 121 strains of yeast (representing 24 genera and 72 species) associated with wine and food fermentations. This database exhibits sensitive discrimination among species and robust intraspecific conservation of TRFLP profiles, enabling reliable characterization of mixed yeast communities. The yeast ecology of sweet, botrytized wine fermentations from two separate vintages was analyzed using this database, demonstrating the utility of this method for fast-paced, qualitative detection and identification of differences in yeast community structures over time in a complex, diverse fermentation system.
650 $aGrapes
650 $aFermentação
650 $aUva
650 $aVinho
650 $aVitis Vinifera
653 $aLevedura
653 $aTRFLP
700 1 $aHWANG, C. F.
700 1 $aLIU, S.
700 1 $aMILLS, K. L. B.
700 1 $aMILLS, D. A.
773 $tAmerican Journal of Enology and Viticulture, Reedley$gv. 63, n. 2, p. 185-194, 2012.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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