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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
19/09/2018 |
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Data da última atualização: |
28/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, L. L.; PESTANA, K. N.; FERREIRA, C. F.; OLIVEIRA, S. A. S. de. |
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Afiliação: |
LEANDRO L. SILVA, UFRB; KÁTIA N. PESTANA; CLAUDIA FORTES FERREIRA, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
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Título: |
Differentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v.43, p.194-20, 2018. |
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ISSN: |
1983-2052 |
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Idioma: |
Inglês |
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Conteúdo: |
Different species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing. |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01935naa a2200193 a 4500
001 2096059
005 2018-09-28
008 2018 bl uuuu u00u1 u #d
022 $a1983-2052
100 1 $aSILVA, L. L.
245 $aDifferentiation of phylogenetic lineages within the 'Colletotrichum gloeosporioides species complex' associated with cassava anthracnose disease by PCR-RFLP.$h[electronic resource]
260 $c2018
520 $aDifferent species were found associated with cassava anthracnose, considered a major disease of this crop. The correct identification of the causal agent is a first step for defining appropriate control strategies, such as resistant varieties. In silico analyses used sequences of six genomic regions of ex-type specimens from the ?Colletotrichum gloeosporioides species complex? (C.g.SC) and 21 restriction enzymes to identify phylogenetic lineages. The three best combinations of region/enzymes were validated in 18 Colletotrichum spp. isolates from cassava. Dendrograms for in silico PCR-RFLP for CAL, ITS and TUB2 showed considerable agreement with the phylogenetic analysis of each genomic region; however, the CAL gene presented greater discriminatory power. Since the band patterns from in gel analysis were almost the same as expected for the in silico analysis for the CAL region, a new approach was proposed based on the combined data from these two methodologies, allowing the differentiation of five phylogenetic lineages within the C.g.SC (C. tropicale; C. fructicola; C. siamense; C. gloeosporioides sensu stricto and C. theobromicola), and one outside of this complex (C. cliviae). This evaluation showed to be a reliable technique for preliminary identification of species prior to sequencing.
650 $aCassava
650 $aMandioca
700 1 $aPESTANA, K. N.
700 1 $aFERREIRA, C. F.
700 1 $aOLIVEIRA, S. A. S. de
773 $tTropical Plant Pathology$gv.43, p.194-20, 2018.
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